Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1983 Sep;24(3):450–452. doi: 10.1128/aac.24.3.450

Comparative analysis of conjugative plasmids mediating gentamicin resistance in Staphylococcus aureus.

R V Goering, E A Ruff
PMCID: PMC185346  PMID: 6639004

Abstract

Five gentamicin-resistant clinical isolates of Staphylococcus aureus were found to contain self-transmissible plasmids of 32 to 37 megadaltons in size. Restriction endonuclease digests of the plasmids were markedly similar to those of reference plasmids of unrelated geographical origin, thus suggesting the significant contribution of common conjugal plasmids to the emergence of gentamicin resistance in S. aureus populations.

Full text

PDF
450

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen M. L., Wong E. S., Falkow S. Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak. Antimicrob Agents Chemother. 1982 Feb;21(2):210–215. doi: 10.1128/aac.21.2.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Engel H. W., Soedirman N., Rost J. A., van Leeuwen W. J., van Embden J. D. Transferability of macrolide, lincomycin, and streptogramin resistances between group A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus. J Bacteriol. 1980 May;142(2):407–413. doi: 10.1128/jb.142.2.407-413.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forbes B. A., Schaberg D. R. Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. J Bacteriol. 1983 Feb;153(2):627–634. doi: 10.1128/jb.153.2.627-634.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holmes D. S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem. 1981 Jun;114(1):193–197. doi: 10.1016/0003-2697(81)90473-5. [DOI] [PubMed] [Google Scholar]
  5. KLOSS W. E., PATTEE P. A. TRANSDUCTION ANALYSIS OF THE HISTIDINE REGION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1965 May;39:195–207. doi: 10.1099/00221287-39-2-195. [DOI] [PubMed] [Google Scholar]
  6. McDonnell R. W., Sweeney H. M., Cohen S. Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother. 1983 Jan;23(1):151–160. doi: 10.1128/aac.23.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PATTEE P. A., BALDWIN J. N. Transduction of resistance to chlortetracycline and novobiocin in Staphylococcus aureus. J Bacteriol. 1961 Dec;82:875–881. doi: 10.1128/jb.82.6.875-881.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PATTEE P. A., BALDWIN J. N. Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus. J Bacteriol. 1962 Nov;84:1049–1055. doi: 10.1128/jb.84.5.1049-1055.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schaberg D. R., Clewell D. B., Glatzer L. Conjugative transfer of R-plasmids from Streptococcus faecalis to Staphylococcus aureus. Antimicrob Agents Chemother. 1982 Aug;22(2):204–207. doi: 10.1128/aac.22.2.204. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES