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. 1987 Jun;91(2):333–346. doi: 10.1111/j.1476-5381.1987.tb10288.x

An investigation of the role of adrenergic innervation in the regulation of the extraneuronal uptake of [3H]-isoprenaline into rat vasa deferentia and atria.

A J Morton
PMCID: PMC1853508  PMID: 3607361

Abstract

Destruction of adrenergic nerves by neonatal guanethidine treatment did not affect the extraneuronal accumulation of [3H]-isoprenaline by adult vasa deferentia or atria. Neither pre- nor post-ganglionic denervation of vasa deferentia resulted in a significant change in extraneuronal accumulation of [3H]-isoprenaline. The appearance and subsequent development of extraneuronal uptake in embryonic and newborn rats did not appear to be dependent on a fully developed or functional adrenergic innervation. Decreasing plasma catecholamine levels by adrenal demedullation did not have an effect on extraneuronal uptake. Chronic cold exposure (7 days) significantly increased plasma levels of adrenaline, and there was a significant decrease in the corticosterone-sensitive extraneuronal uptake of [3H]-isoprenaline into atria. After 14 days in the cold, plasma adrenaline levels had fallen, and were no longer significantly different from control levels. The extraneuronal uptake of [3H]-isoprenaline into atria after 14 days cold exposure was no longer different from that of control atria. There did not appear to be a direct correlation between plasma catecholamines and extraneuronal accumulation of [3H]-isoprenaline. Neither the presence of adrenergic nerves nor plasma catecholamines appear to play a major role in the regulation of extraneuronal uptake by atria and vasa deferentia of the rat.

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Selected References

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  1. Anning E. N., O'Donnell S. R., Wanstall J. C., Hughes I. E. Extraneuronal accumulation of isoprenaline in guinea-pig trachea, atria and uterus: a histochemical and pharmacological study [proceedings]. Br J Pharmacol. 1978 Mar;62(3):472P–472P. [PMC free article] [PubMed] [Google Scholar]
  2. Benedict C. R., Fillenz M., Stanford C. Noradrenaline release in rats during prolonged cold-stress and repeated swim-stress. Br J Pharmacol. 1979 Aug;66(4):521–524. doi: 10.1111/j.1476-5381.1979.tb13689.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennett T., Gardiner S. M., Kemp P. A. An assessment of the effectiveness of neonatal treatment with guanethidine as a means of producing sympathectomy. Br J Pharmacol. 1982 Aug;76(4):557–564. doi: 10.1111/j.1476-5381.1982.tb09254.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birmingham A. T. Sympathetic denervation of the smooth muscle of the vas deferens. J Physiol. 1970 Mar;206(3):645–661. doi: 10.1113/jphysiol.1970.sp009035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bralet J., Beley A., Lallemant A. M. Modifications du taux de renouvellement de la noradrénaline dans différents organes périphériques du rat au cours de l'exposition et de l'acclimatation au froid. Pflugers Arch. 1972;335(3):186–197. doi: 10.1007/BF00592156. [DOI] [PubMed] [Google Scholar]
  6. Burnstock G., McCulloch M. W., Story D. F., Wright M. E. Factors affecting the extraneuronal inactivation of noradrenaline in cardiac and smooth muscle. Br J Pharmacol. 1972 Oct;46(2):243–253. doi: 10.1111/j.1476-5381.1972.tb06869.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Callingham B. A., Burgen A. S. The uptake of isoprenaline and noradrenaline by the perfused rat heart. Mol Pharmacol. 1966 Jan;2(1):37–42. [PubMed] [Google Scholar]
  8. Clark D. W., Laverty R., Phelan E. L. Long-lasting peripheral and central effects of 6-hydroxydopamine in rats. Br J Pharmacol. 1972 Feb;44(2):233–243. doi: 10.1111/j.1476-5381.1972.tb07259.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. De Champlain J., Malmfors T., Olson L., Sachs C. Ontogenesis of peripheral adrenergic neurons in the rat: pre- and postnatal observations. Acta Physiol Scand. 1970 Oct;80(2):276–288. doi: 10.1111/j.1748-1716.1970.tb04791.x. [DOI] [PubMed] [Google Scholar]
  10. FLEMING W. W., TRENDELENBURG U. The development of supersensitivity to norepinephrine after pretreatment with reserpine. J Pharmacol Exp Ther. 1961 Jul;133:41–51. [PubMed] [Google Scholar]
  11. Garver D. L., Cedarbaum J., Maas J. W. Blood-brain barrier to 6-hydroxydopamine: uptake by heart and brain. Life Sci. 1975 Oct 10;17(7):1081–1084. doi: 10.1016/0024-3205(75)90328-8. [DOI] [PubMed] [Google Scholar]
  12. Gillespie J. S., Hamilton D. N., Hosie J. A. The extraneuronal uptake and localization of noradrenaline in the cat spleen and the effect on this of some drugs, of cold and of denervation. J Physiol. 1970 Mar;206(3):563–590. doi: 10.1113/jphysiol.1970.sp009031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gillespie J. S., Muir T. C. Species and tissue variation in extraneuronal and neuronal accumulation of noradrenaline. J Physiol. 1970 Mar;206(3):591–604. doi: 10.1113/jphysiol.1970.sp009032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HERTTING G. THE FATE OF 3H-ISO-PROTERENOL IN THE RAT. Biochem Pharmacol. 1964 Aug;13:1119–1128. doi: 10.1016/0006-2952(64)90112-1. [DOI] [PubMed] [Google Scholar]
  15. Head R. J., De La Lande I. S., Irvine R. J., Johnson S. M. Uptake and O-methylation of isoprenaline in the rabbit ear artery. Blood Vessels. 1980;17(5):229–245. doi: 10.1159/000158253. [DOI] [PubMed] [Google Scholar]
  16. Iversen L. L., De Champlain J., Glowinski J., Axelrod J. Uptake, storage and metabolism of norepinephrine in tissues of the developing rat. J Pharmacol Exp Ther. 1967 Sep;157(3):509–516. [PubMed] [Google Scholar]
  17. Jacks B. R., de Champlain J., Cordeau J. P. Effects of 6-hydroxydopamine on putative transmitter substances in the central nervous system. Eur J Pharmacol. 1972 May;18(3):353–360. doi: 10.1016/0014-2999(72)90036-2. [DOI] [PubMed] [Google Scholar]
  18. Jarrott B. Uptake and metabolism of catecholamines in the perfused hearts of different species. Br J Pharmacol. 1970 Apr;38(4):810–821. doi: 10.1111/j.1476-5381.1970.tb09890.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Johnson G. E., Pritzker K. The influence of the dopa decarboxylase inhibitor Ro4-4602 on the urinary excretion of catecholamines in cold-stressed rats. J Pharmacol Exp Ther. 1966 Jun;152(3):432–438. [PubMed] [Google Scholar]
  20. Kasuya Y., Goto K., Hashimoto H., Watanabe H., Munakata H., Watanabe M. Nonspecific denervation supersensitivity in the rat vas deferens "in vitro". Eur J Pharmacol. 1969 Nov;8(2):177–184. doi: 10.1016/0014-2999(69)90074-0. [DOI] [PubMed] [Google Scholar]
  21. Keller R., Oke A., Mefford I., Adams R. N. Liquid chromatographic analysis of catecholamines routine assay for regional brain mapping. Life Sci. 1976 Oct 1;19(7):995–1003. doi: 10.1016/0024-3205(76)90290-3. [DOI] [PubMed] [Google Scholar]
  22. LEDUC J. Catecholamine production and release in exposure and acclimation to cold. Acta Physiol Scand Suppl. 1961;183:1–101. [PubMed] [Google Scholar]
  23. LEDUC J. Excretion of catecholamines in rats exposed to cold. Acta Physiol Scand. 1961 Jan;51:94–95. doi: 10.1111/j.1748-1716.1961.tb02117.x. [DOI] [PubMed] [Google Scholar]
  24. Mefford I. N. Application of high performance liquid chromatography with electrochemical detection to neurochemical analysis: measurement of catecholamines, serotonin and metabolites in rat brain. J Neurosci Methods. 1981 Feb;3(3):207–224. doi: 10.1016/0165-0270(81)90056-x. [DOI] [PubMed] [Google Scholar]
  25. Morton A. J. The effect of reserpine treatment on the extraneuronal uptake of [3H]-isoprenaline into rat atria. Br J Pharmacol. 1985 Sep;86(1):287–295. doi: 10.1111/j.1476-5381.1985.tb09460.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Nikodejevic B., Senoh S., Daly J. W., Creveling C. R. Catechol-O-methyltransferase. II. A new class of inhibitors of catechol-o-methyltransferase; 3,5-dihydroxy-4-methoxybenzoic acid and related compounds. J Pharmacol Exp Ther. 1970 Jul;174(1):83–93. [PubMed] [Google Scholar]
  27. SJOSTRAND N. O. Effect of reserpine and hypogastric denervation on the noradrenaline content of the vas deferens and the seminal vesicle of the guinea-pig. Acta Physiol Scand. 1962 Nov-Dec;56:376–380. doi: 10.1111/j.1748-1716.1962.tb02513.x. [DOI] [PubMed] [Google Scholar]
  28. Salt P. J. Inhibition of noradrenaline uptake 2 in the isolated rat heart by steroids, clonidine and methoxylated phenylethylamines. Eur J Pharmacol. 1972 Dec;20(3):329–340. doi: 10.1016/0014-2999(72)90194-x. [DOI] [PubMed] [Google Scholar]
  29. Salt P. J., Iversen L. L. Catecholamine uptake sites in the rat heart after 6-hydroxydopamine treatment and in a genetically hypertensive strain. Naunyn Schmiedebergs Arch Pharmacol. 1973;279(4):381–386. doi: 10.1007/BF00500803. [DOI] [PubMed] [Google Scholar]
  30. Schiebler T. H., Heene R. Nachweis von Katecholaminen im Rattenherzen während der Entwicklung. Histochemie. 1968;14(4):328–334. doi: 10.1007/BF00304256. [DOI] [PubMed] [Google Scholar]
  31. Torre J. C., Surgeon J. W. A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method. Histochemistry. 1976 Oct 22;49(2):81–93. doi: 10.1007/BF00495672. [DOI] [PubMed] [Google Scholar]
  32. Uhlig W., Fiebig R., Trendelenburg U. The effect of corticosterone on the fluxes of 3H-normetanephrine into and out of the extraneuronal compartments of the perfused rat heart. Naunyn Schmiedebergs Arch Pharmacol. 1976 Oct;295(1):45–50. doi: 10.1007/BF00509771. [DOI] [PubMed] [Google Scholar]

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