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. 1987 Aug;91(4):857–869. doi: 10.1111/j.1476-5381.1987.tb11285.x

Type 1 hypersensitivity reactions in reconstructed tissues using syngeneic cell types.

A W Baird 1, A W Cuthbert 1, L J MacVinish 1
PMCID: PMC1853577  PMID: 3664081

Abstract

1. Type 1 hypersensitivity reactions in response to antigen challenge have been measured as short circuit current (SCC) responses in reconstructed tissues consisting of syngeneic cell types. 2. In all instances reconstructed tissues consisted of an epithelial monolayer grown on collagen-coated Millipore filters and a pad of peritoneal cells. Monolayers were formed of either HCA-7 or HCA-7-Col 1 cells derived from a human adenocarcinoma. Peritoneal cells were derived from rats or guinea-pigs sensitized to either ovalbumin or beta-lactoglobulin. 3. The SCC responses of the monolayers were dependent upon the 'concentration' of peritoneal cells in the reconstructed tissue. The threshold concentration was 0.4 X 10(6) cells when rat peritoneal cells are combined with an epithelial monolayer of 0.2 cm2. 4. The SCC responses in response to antigen challenge were selectively inhibited by the H1-receptor antagonist, mepyramine. Similarly the effects of exogenously applied histamine were antagonised by mepyramine. 5. The responses to antigen challenge were not inhibited by tetrodotoxin in reconstructed tissues. This result is in contrast to that with isolated intestinal epithelia from sensitized animals where tetrodotoxin inhibits the SCC responses to external field stimulation and to challenge with antigens. The consequences of these results for understanding the mechanisms of epithelial Type 1 hypersensitivity reactions are discussed. Suggestions are made to illustrate how the methods developed here may be employed to ask questions about the nature of mediators released and the types of cell responsible in human disease conditions.

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Selected References

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  1. Atkinson G., Ennis M., Pearce F. L. The effect of alkaline earth cations on the release of histamine from rat peritoneal mast cells treated with compound 48/80 and peptide 401. Br J Pharmacol. 1979 Mar;65(3):395–402. doi: 10.1111/j.1476-5381.1979.tb07843.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baird A. W., Barclay W. S., Blazer-Yost B. L., Cuthbert A. W. Affinity purified immunoglobulin G transfers immediate hypersensitivity to guinea pig colonic epithelium in vitro. Gastroenterology. 1987 Mar;92(3):635–642. doi: 10.1016/0016-5085(87)90011-4. [DOI] [PubMed] [Google Scholar]
  3. Baird A. W., Cuthbert A. W., Pearce F. L. Immediate hypersensitivity reactions in epithelia from rats infected with Nippostrongylus brasiliensis. Br J Pharmacol. 1985 Aug;85(4):787–795. doi: 10.1111/j.1476-5381.1985.tb11077.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Befus A. D., Pearce F. L., Gauldie J., Horsewood P., Bienenstock J. Mucosal mast cells. I. Isolation and functional characteristics of rat intestinal mast cells. J Immunol. 1982 Jun;128(6):2475–2480. [PubMed] [Google Scholar]
  5. Castro G. A. Immunological regulation of epithelial function. Am J Physiol. 1982 Nov;243(5):G321–G329. doi: 10.1152/ajpgi.1982.243.5.G321. [DOI] [PubMed] [Google Scholar]
  6. Cuthbert A. W., Kirkland S. C., MacVinish L. J. Kinin effects on ion transport in monolayers of HCA-7 cells, a line from a human colonic adenocarcinoma. Br J Pharmacol. 1985 Sep;86(1):3–5. doi: 10.1111/j.1476-5381.1985.tb09428.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cuthbert A. W., Margolius H. S. Kinins stimulate net chloride secretion by the rat colon. Br J Pharmacol. 1982 Apr;75(4):587–598. doi: 10.1111/j.1476-5381.1982.tb09178.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cuthbert A. W., McLaughlan P., Coombs R. R. Immediate hypersensitivity reaction to beta-lactoglobulin in the epithelium lining the colon of guinea pigs fed cows' milk. Int Arch Allergy Appl Immunol. 1983;72(1):34–40. doi: 10.1159/000234837. [DOI] [PubMed] [Google Scholar]
  9. Dale M. M., Zilletti L. The Schultz-Dale response of the longitudinal muscle strip preparation of guinea-pig ileum. Br J Pharmacol. 1970 Jul;39(3):542–545. doi: 10.1111/j.1476-5381.1970.tb10362.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dharmsathaphorn K., McRoberts J. A., Mandel K. G., Tisdale L. D., Masui H. A human colonic tumor cell line that maintains vectorial electrolyte transport. Am J Physiol. 1984 Feb;246(2 Pt 1):G204–G208. doi: 10.1152/ajpgi.1984.246.2.G204. [DOI] [PubMed] [Google Scholar]
  11. Foreman J. C., Piotrowski W. Peptides and histamine release. J Allergy Clin Immunol. 1984 Aug;74(2):127–131. doi: 10.1016/0091-6749(84)90274-4. [DOI] [PubMed] [Google Scholar]
  12. Grasset E., Pinto M., Dussaulx E., Zweibaum A., Desjeux J. F. Epithelial properties of human colonic carcinoma cell line Caco-2: electrical parameters. Am J Physiol. 1984 Sep;247(3 Pt 1):C260–C267. doi: 10.1152/ajpcell.1984.247.3.C260. [DOI] [PubMed] [Google Scholar]
  13. Handler J. S. Use of cultured epithelia to study transport and its regulation. J Exp Biol. 1983 Sep;106:55–69. doi: 10.1242/jeb.106.1.55. [DOI] [PubMed] [Google Scholar]
  14. Hubel K. A. Electrical stimulus-secretion coupling in rabbit ileal mucosa. J Pharmacol Exp Ther. 1984 Dec;231(3):577–582. [PubMed] [Google Scholar]
  15. Kazimierczak W., Diamant B. Mechanisms of histamine release in anaphylactic and anaphylactoid reactions. Prog Allergy. 1978;24:295–365. [PubMed] [Google Scholar]
  16. Lazarus S. C., McCabe L. J., Nadel J. A., Gold W. M., Leikauf G. D. Effects of mast cell-derived mediators on epithelial cells in canine trachea. Am J Physiol. 1986 Sep;251(3 Pt 1):C387–C394. doi: 10.1152/ajpcell.1986.251.3.C387. [DOI] [PubMed] [Google Scholar]
  17. Leung K. B., Flint K. C., Brostoff J., Hudspith B. N., Johnson N. M., Pearce F. L. Some properties of mast cells obtained by human bronchoalveolar lavage. Agents Actions. 1986 Apr;18(1-2):110–112. doi: 10.1007/BF01987997. [DOI] [PubMed] [Google Scholar]
  18. Luzzi S., Franchi-Micheli S., Spagnesi S., Rosi E., Ciuffi M., Zilletti L. Inhibition of anaphylactic histamine release in vitro by GABA. Agents Actions. 1985 Apr;16(3-4):160–162. doi: 10.1007/BF01983127. [DOI] [PubMed] [Google Scholar]
  19. Nosál R., Pecivova J., Drábiková K. Evidence for intracellular histamine liberation in isolated rat mast cells. Agents Actions. 1982 Dec;12(5-6):601–607. doi: 10.1007/BF01965067. [DOI] [PubMed] [Google Scholar]
  20. PADAWER J., GORDON A. S. Cellular elements in the peritoneal fluid of some mammals. Anat Rec. 1956 Feb;124(2):209–222. doi: 10.1002/ar.1091240206. [DOI] [PubMed] [Google Scholar]
  21. Perdue M. H., Forstner J. F., Roomi N. W., Gall D. G. Epithelial response to intestinal anaphylaxis in rats: goblet cell secretion and enterocyte damage. Am J Physiol. 1984 Dec;247(6 Pt 1):G632–G637. doi: 10.1152/ajpgi.1984.247.6.G632. [DOI] [PubMed] [Google Scholar]
  22. Proud D., Togias A., Naclerio R. M., Crush S. A., Norman P. S., Lichtenstein L. M. Kinins are generated in vivo following nasal airway challenge of allergic individuals with allergen. J Clin Invest. 1983 Nov;72(5):1678–1685. doi: 10.1172/JCI111127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Russell D. A., Castro G. A. Anaphylactic-like reaction of small intestinal epithelium in parasitized guinea-pigs. Immunology. 1985 Mar;54(3):573–579. [PMC free article] [PubMed] [Google Scholar]
  24. Welsh M. J. An apical-membrane chloride channel in human tracheal epithelium. Science. 1986 Jun 27;232(4758):1648–1650. doi: 10.1126/science.2424085. [DOI] [PubMed] [Google Scholar]
  25. Widdicombe J. H., Welsh M. J., Finkbeiner W. E. Cystic fibrosis decreases the apical membrane chloride permeability of monolayers cultured from cells of tracheal epithelium. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6167–6171. doi: 10.1073/pnas.82.18.6167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yankaskas J. R., Cotton C. U., Knowles M. R., Gatzy J. T., Boucher R. C. Culture of human nasal epithelial cells on collagen matrix supports. A comparison of bioelectric properties of normal and cystic fibrosis epithelia. Am Rev Respir Dis. 1985 Dec;132(6):1281–1287. doi: 10.1164/arrd.1985.132.6.1281. [DOI] [PubMed] [Google Scholar]

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