Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1987 Aug;91(4):799–802. doi: 10.1111/j.1476-5381.1987.tb11278.x

Bronchial and vascular effects of Paf in the rat isolated lung are completely blocked by WEB 2086, a novel specific Paf antagonist.

J Casals-Stenzel 1, J Franke 1, T Friedrich 1, J Lichey 1
PMCID: PMC1853578  PMID: 3664079

Abstract

1 The effect of the platelet-activating factor (Paf) antagonist, WEB 2086, on Paf-induced increase of pulmonary artery perfusion pressure (Pp), bronchial inflation pressure (Pi) and wet-to-dry lung weight ratios (W/D) was investigated in the rat isolated lung. 2 Lungs were perfused with Krebs-Ringer solution (KRS) as controls or with KRS containing WEB 2086 (0.1, 1.0, 10.0 or 100 micrograms ml-1) and then injected with a bolus of 20 micrograms Paf. 3 A dose-related inhibition of the Paf-induced increase of Pp, Pi and W/D was observed, being almost maximal for the 10.0 micrograms ml-1 and complete for the 100 micrograms ml-1 doses of WEB 2086 when compared to controls. 4 It is concluded that WEB 2086 is a highly effective and specific Paf antagonist in the pulmonary vasculature and bronchial tract.

Full text

PDF
799

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cuss F. M., Dixon C. M., Barnes P. J. Effects of inhaled platelet activating factor on pulmonary function and bronchial responsiveness in man. Lancet. 1986 Jul 26;2(8500):189–192. doi: 10.1016/s0140-6736(86)92489-x. [DOI] [PubMed] [Google Scholar]
  2. Denjean A., Arnoux B., Masse R., Lockhart A., Benveniste J. Acute effects of intratracheal administration of platelet-activating factor in baboons. J Appl Physiol Respir Environ Exerc Physiol. 1983 Sep;55(3):799–804. doi: 10.1152/jappl.1983.55.3.799. [DOI] [PubMed] [Google Scholar]
  3. Doebber T. W., Wu M. S., Robbins J. C., Choy B. M., Chang M. N., Shen T. Y. Platelet activating factor (PAF) involvement in endotoxin-induced hypotension in rats. Studies with PAF-receptor antagonist kadsurenone. Biochem Biophys Res Commun. 1985 Mar 29;127(3):799–808. doi: 10.1016/s0006-291x(85)80014-0. [DOI] [PubMed] [Google Scholar]
  4. Halonen M., Palmer J. D., Lohman I. C., McManus L. M., Pinckard R. N. Differential effects of platelet depletion on the physiologic alterations of IgE anaphylaxis and acetyl glyceryl ether phosphorylcholine infusion in the rabbit. Am Rev Respir Dis. 1981 Oct;124(4):416–421. doi: 10.1164/arrd.1981.124.4.416. [DOI] [PubMed] [Google Scholar]
  5. Hamasaki Y., Mojarad M., Saga T., Tai H. H., Said S. I. Platelet-activating factor raises airway and vascular pressures and induces edema in lungs perfused with platelet-free solution. Am Rev Respir Dis. 1984 May;129(5):742–746. doi: 10.1164/arrd.1984.129.5.742. [DOI] [PubMed] [Google Scholar]
  6. Hauge A. Role of histamine in hypoxic pulmonary hypertension in the rat. I. Blockade or potentiation of endogenous amines, kinins, and ATP. Circ Res. 1968 Mar;22(3):371–383. doi: 10.1161/01.res.22.3.371. [DOI] [PubMed] [Google Scholar]
  7. Lichey J., Friedrich T., Franke J., Nigam S., Priesnitz M., Oeff K. Pressure effects and uptake of platelet-activating factor in isolated rat lung. J Appl Physiol Respir Environ Exerc Physiol. 1984 Oct;57(4):1039–1044. doi: 10.1152/jappl.1984.57.4.1039. [DOI] [PubMed] [Google Scholar]
  8. Mojarad M., Hamasaki Y., Said S. I. Platelet-activating factor increases pulmonary microvascular permeability and induces pulmonary edema. A preliminary report. Bull Eur Physiopathol Respir. 1983 May-Jun;19(3):253–256. [PubMed] [Google Scholar]
  9. Pinckard R. N., Farr R. S., Hanahan D. J. Physicochemical and functional identity of rabbit platelet-activating factor (PAF) released in vivo during IgE anaphylaxis with PAF released in vitro from IgE sensitized basophils. J Immunol. 1979 Oct;123(4):1847–1857. [PubMed] [Google Scholar]
  10. Terashita Z., Imura Y., Nishikawa K., Sumida S. Is platelet activating factor (PAF) a mediator of endotoxin shock? Eur J Pharmacol. 1985 Feb 26;109(2):257–261. doi: 10.1016/0014-2999(85)90427-3. [DOI] [PubMed] [Google Scholar]
  11. Voelkel N. F., Worthen S., Reeves J. T., Henson P. M., Murphy R. C. Nonimmunological production of leukotrienes induced by platelet-activating factor. Science. 1982 Oct 15;218(4569):286–289. doi: 10.1126/science.7123233. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES