Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1987 Aug;91(4):849–856. doi: 10.1111/j.1476-5381.1987.tb11284.x

Effects of pulmonary oedema on pharmacokinetics of adenosine in rat isolated lungs.

Y S Bakhle 1, C J Grantham 1
PMCID: PMC1853583  PMID: 3117151

Abstract

1 Pulmonary oedema, assessed by decreases in the lung dry weight:wet weight ratio, was induced in rats by a single i.p. injection of alpha-naphthylthiourea (ANTU). The oedema reached a peak at 4 h after ANTU and had completely resolved after 28 h. 2 Pulmonary pharmacokinetics of adenosine were measured in isolated, perfused lungs using radiolabelled adenosine and sucrose, injected into the perfusate as a single bolus. 3 By 1 h after ANTU the 1 min efflux of tracer for adenosine increased to over 60% and remained high until 16 h after ANTU. The time for 50% of injected radioactivity to appear in lung effluent (t1/2) for adenosine was reduced from its normal value of greater than 120 s to a minimum of 27 s at 1 h after ANTU. The proportion of adenosine in lung effluent did not change until 16 h after ANTU treatment but returned to normal by 50 h. 4 There were only minimal changes in the T1/2 and 1 min efflux for sucrose following ANTU treatment. 5 It appears that both the uptake and metabolism of adenosine are affected by ANTU-induced lung damage. The early effects are chiefly on uptake with metabolism remaining normal. Later (after 16 h) metabolism is decreased with uptake recovering to normal levels. 6 The effects on adenosine uptake paralleled the development and the resolution of oedema, suggesting that this variable might provide a biochemical index of the physical processes leading to lung oedema.

Full text

PDF
849

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali A. E., Barrett J. C., Eling T. E. Prostaglandin and thromboxane production by fibroblasts and vascular endothelial cells. Prostaglandins. 1980 Oct;20(4):667–688. doi: 10.1016/0090-6980(80)90107-0. [DOI] [PubMed] [Google Scholar]
  2. Andy R. J., Kornfeld R. The adenosine deaminase binding protein of human skin fibroblasts is located on the cell surface. J Biol Chem. 1982 Jul 25;257(14):7922–7925. [PubMed] [Google Scholar]
  3. Bakhle Y. S., Chelliah R. Metabolism and uptake of adenosine in rat isolated lung and its inhibition. Br J Pharmacol. 1983 Jun;79(2):509–515. doi: 10.1111/j.1476-5381.1983.tb11025.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bakhle Y. S. Decreased inactivation of prostaglandin E2 in isolated lungs from rats with alpha-naphthyl thiourea-induced pulmonary oedema. Biochem Pharmacol. 1982 Nov 1;31(21):3395–3401. doi: 10.1016/0006-2952(82)90617-7. [DOI] [PubMed] [Google Scholar]
  5. Bakhle Y. S., Grantham C. J. Selective effect of pulmonary oedema on prostaglandin E2 pharmacokinetics in rat lung. Biochem Pharmacol. 1985 Dec 15;34(24):4325–4327. doi: 10.1016/0006-2952(85)90292-8. [DOI] [PubMed] [Google Scholar]
  6. Bakhle Y. S., Reynard A. M., Vane J. R. Metabolism of the angiotensins in isolated perfused tissues. Nature. 1969 Jun 7;222(5197):956–959. doi: 10.1038/222956a0. [DOI] [PubMed] [Google Scholar]
  7. Block E. R., Schoen F. J. Effect of alpha naphthylthiourea on uptake of 5-hydroxytryptamine from the pulmonary circulation. Am Rev Respir Dis. 1981 Jan;123(1):69–73. doi: 10.1164/arrd.1981.123.1.69. [DOI] [PubMed] [Google Scholar]
  8. Cunningham A. L., Hurley J. V. Alpha-naphthyl-thiourea-induced pulmonary oedema in the rat: a topographical and electron-microscope study. J Pathol. 1972 Jan;106(1):25–35. doi: 10.1002/path.1711060103. [DOI] [PubMed] [Google Scholar]
  9. Dieterle Y., Ody C., Ehrensberger A., Stalder H., Junod A. F. Metabolism and uptake of adenosine triphosphate and adenosine by porcine aortic and pulmonary endothelial cells and fibroblasts in culture. Circ Res. 1978 Jun;42(6):869–876. doi: 10.1161/01.res.42.6.869. [DOI] [PubMed] [Google Scholar]
  10. Fox R. B., Harada R. N., Tate R. M., Repine J. E. Prevention of thiourea-induced pulmonary edema by hydroxyl-radical scavengers. J Appl Physiol Respir Environ Exerc Physiol. 1983 Nov;55(5):1456–1459. doi: 10.1152/jappl.1983.55.5.1456. [DOI] [PubMed] [Google Scholar]
  11. Hellewell P. G., Pearson J. D. Metabolism of circulating adenosine by the porcine isolated perfused lung. Circ Res. 1983 Jul;53(1):1–7. doi: 10.1161/01.res.53.1.1. [DOI] [PubMed] [Google Scholar]
  12. Meyrick B., Miller J., Reid L. Pulmonary oedema induced by ANTU, or by high or low oxygen concentrations in rat--an electron microscopic study. Br J Exp Pathol. 1972 Aug;53(4):347–358. [PMC free article] [PubMed] [Google Scholar]
  13. Minty B. D., Scudder C. M., Grantham C. J., Jones J. G., Bakhle Y. S. Sequential changes in lung metabolism, permeability, and edema after ANTU. J Appl Physiol (1985) 1987 Feb;62(2):491–496. doi: 10.1152/jappl.1987.62.2.491. [DOI] [PubMed] [Google Scholar]
  14. Moncada S., Vane J. R. Prostacyclin and the vascular endothelium. Bull Eur Physiopathol Respir. 1981;17(4):687–701. [PubMed] [Google Scholar]
  15. Ody C., Dieterle Y., Wand I., Stalder H., Junod A. F. PGA1 and PGF2 alpha metabolism by pig pulmonary endothelium, smooth muscle, and fibroblasts. J Appl Physiol Respir Environ Exerc Physiol. 1979 Feb;46(2):211–216. doi: 10.1152/jappl.1979.46.2.211. [DOI] [PubMed] [Google Scholar]
  16. Pankhania J. J., Bakhle Y. S. Effect of pulmonary oedema induced by alpha-naphthylthiourea on synthesis of cyclo-oxygenase products in rat isolated lungs. Prostaglandins. 1985 Jul;30(1):37–49. doi: 10.1016/s0090-6980(85)80009-5. [DOI] [PubMed] [Google Scholar]
  17. Pearson J. D., Carleton J. S., Hutchings A., Gordon J. L. Uptake and metabolism of adenosine by pig aortic endothelial and smooth-muscle cells in culture. Biochem J. 1978 Feb 15;170(2):265–271. doi: 10.1042/bj1700265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rubio V. R., Wiedmeier T., Berne R. M. Nucleoside phosphorylase: localization and role in the myocardial distribution of purines. Am J Physiol. 1972 Mar;222(3):550–555. doi: 10.1152/ajplegacy.1972.222.3.550. [DOI] [PubMed] [Google Scholar]
  19. Shimizu H., Creveling C. R., Daly J. Stimulated formation of adenosine 3',5'-cyclic phosphate in cerebral cortex: synergism between electrical activity and biogenic amines. Proc Natl Acad Sci U S A. 1970 Apr;65(4):1033–1040. doi: 10.1073/pnas.65.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Staub N. C. Pulmonary edema. Physiol Rev. 1974 Jul;54(3):678–811. doi: 10.1152/physrev.1974.54.3.678. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES