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. 1987 Aug;91(4):729–733. doi: 10.1111/j.1476-5381.1987.tb11270.x

Endothelium and the vasodilator action of rat calcitonin gene-related peptide (CGRP).

G C Grace 1, G J Dusting 1, B E Kemp 1, T J Martin 1
PMCID: PMC1853586  PMID: 2822189

Abstract

1 Acetylcholine and rat calcitonin gene-related peptide (CGRP) produced a relaxation in rat isolated aortic rings which was entirely dependent on the presence of the endothelium. 2 In the absence of any exogenous vasodilator agent, the cyclic guanosine monophosphate (cyclic GMP) content was higher in rings with endothelium than in those without. 3 The vasorelaxation produced by acetylcholine and sodium nitroprusside was accompanied by increases in cyclic GMP in the smooth muscle, whereas that produced by CGRP was not accompanied by cyclic GMP accumulation. 4 Therefore, it appears unlikely that CGRP releases an endothelium-derived relaxing factor similar to that released by acetylcholine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Brain S. D., Williams T. J., Tippins J. R., Morris H. R., MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature. 1985 Jan 3;313(5997):54–56. doi: 10.1038/313054a0. [DOI] [PubMed] [Google Scholar]
  3. Furchgott R. F. Role of endothelium in responses of vascular smooth muscle. Circ Res. 1983 Nov;53(5):557–573. doi: 10.1161/01.res.53.5.557. [DOI] [PubMed] [Google Scholar]
  4. Furchgott R. F., Zawadzki J. V. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 1980 Nov 27;288(5789):373–376. doi: 10.1038/288373a0. [DOI] [PubMed] [Google Scholar]
  5. Hunt N. H., Smith B., Pembrey R. Cyclic nucleotide excretion in human malignancies. Clin Sci (Lond) 1980 Jun;58(6):463–467. doi: 10.1042/cs0580463. [DOI] [PubMed] [Google Scholar]
  6. Ignarro L. J., Kadowitz P. J. The pharmacological and physiological role of cyclic GMP in vascular smooth muscle relaxation. Annu Rev Pharmacol Toxicol. 1985;25:171–191. doi: 10.1146/annurev.pa.25.040185.001131. [DOI] [PubMed] [Google Scholar]
  7. Kamm K. E., Stull J. T. The function of myosin and myosin light chain kinase phosphorylation in smooth muscle. Annu Rev Pharmacol Toxicol. 1985;25:593–620. doi: 10.1146/annurev.pa.25.040185.003113. [DOI] [PubMed] [Google Scholar]
  8. Kubota M., Moseley J. M., Butera L., Dusting G. J., MacDonald P. S., Martin T. J. Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells. Biochem Biophys Res Commun. 1985 Oct 15;132(1):88–94. doi: 10.1016/0006-291x(85)90992-1. [DOI] [PubMed] [Google Scholar]
  9. Marshall I., Al-Kazwini S. J., Holman J. J., Craig R. K. Human and rat alpha-CGRP but not calcitonin cause mesenteric vasodilatation in rats. Eur J Pharmacol. 1986 Apr 16;123(2):217–222. doi: 10.1016/0014-2999(86)90662-x. [DOI] [PubMed] [Google Scholar]
  10. Marshall I., Al-Kazwini S. J., Roberts P. M., Shepperson N. B., Adams M., Craig R. K. Cardiovascular effects of human and rat CGRP compared in the rat and other species. Eur J Pharmacol. 1986 Apr 16;123(2):207–216. doi: 10.1016/0014-2999(86)90661-8. [DOI] [PubMed] [Google Scholar]
  11. Martin W., Furchgott R. F., Villani G. M., Jothianandan D. Depression of contractile responses in rat aorta by spontaneously released endothelium-derived relaxing factor. J Pharmacol Exp Ther. 1986 May;237(2):529–538. [PubMed] [Google Scholar]
  12. Popescu L. M., Panoiu C., Hinescu M., Nutu O. The mechanism of cGMP-induced relaxation in vascular smooth muscle. Eur J Pharmacol. 1985 Jan 8;107(3):393–394. doi: 10.1016/0014-2999(85)90269-9. [DOI] [PubMed] [Google Scholar]
  13. Rapoport R. M., Murad F. Agonist-induced endothelium-dependent relaxation in rat thoracic aorta may be mediated through cGMP. Circ Res. 1983 Mar;52(3):352–357. doi: 10.1161/01.res.52.3.352. [DOI] [PubMed] [Google Scholar]
  14. Rosenfeld M. G., Mermod J. J., Amara S. G., Swanson L. W., Sawchenko P. E., Rivier J., Vale W. W., Evans R. M. Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature. 1983 Jul 14;304(5922):129–135. doi: 10.1038/304129a0. [DOI] [PubMed] [Google Scholar]

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