Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1987 Sep;92(1):31–37. doi: 10.1111/j.1476-5381.1987.tb11292.x

The protective effects of sulphasalazine against ethanol-induced gastric damage in rats.

C H Cho 1, C W Ogle 1, E L Sevilla 1
PMCID: PMC1853607  PMID: 2889494

Abstract

1 The inhibitory action of sulphasalazine on ethanol-induced gastric damage was studied in rats. 2 Sulphasalazine (62.5 or 125 mg kg-1, s.c.) did not affect basal gastric acid secretion but increased pepsin output. 3 Ethanol (40% v/v, 10 ml kg-1, p.o.) produced severe gastric glandular mucosal damage and lessened the stomach emptying rate of resin pellets, but it increased the levels of prostaglandin E2 (PGE2)-like activity in the glandular mucosa. 4 Sulphasalazine markedly prevented ethanol-induced damage and significantly elevated gastric wall mucus levels both in basal conditions and in the presence of ethanol. 5 Sulphasalazine caused a small insignificant increase in mucosal PGE2 levels in both control and ethanol-treated rats. The drug significantly increased mucosal PGE2 levels in indomethacin-treated animals, but did not prevent indomethacin-induced mucosal damage. 6 Sulphapyridine but not 5-aminosalicylic acid, constituents of sulphasalazine, showed a similar antilesion action to the parent drug, and prevented gastric wall mucus depletion in ethanol-treated animals. 7 This study elucidates the protective effects of sulphasalazine against ethanol-induced gastric lesions. The antagonistic action appears to be mediated, at least partly, through the preservation of gastric wall mucus by sulphapyridine.

Full text

PDF
31

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. K., Brashler J. R., Johnson M. A. Inhibition by sulfasalazine of LTC synthetase and of rat liver glutathione S-transferases. Biochem Pharmacol. 1985 Aug 1;34(15):2695–2704. doi: 10.1016/0006-2952(85)90570-2. [DOI] [PubMed] [Google Scholar]
  2. Barrett K. E., Tashof T. L., Metcalfe D. D. Inhibition of IgE-mediated mast cell degranulation by sulphasalazine. Eur J Pharmacol. 1985 Jan 2;107(2):279–281. doi: 10.1016/0014-2999(85)90071-8. [DOI] [PubMed] [Google Scholar]
  3. Bennett A., Melhuish P. B., Stamford I. F. Carbenoxolone and deglycyrrhized liquorice have little or no effect on prostanoid synthesis by rat gastric mucosa ex vivo. Br J Pharmacol. 1985 Nov;86(3):693–695. doi: 10.1111/j.1476-5381.1985.tb08947.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berstad A. A modified hemoglobin substrate method for the estimation of pepsin in gastric juice. Scand J Gastroenterol. 1970;5(5):343–348. [PubMed] [Google Scholar]
  5. Cho C. H., Ogle C. W. A correlative study of the antiulcer effects of zinc sulphate in stressed rats. Eur J Pharmacol. 1978 Mar 1;48(1):97–105. doi: 10.1016/0014-2999(78)90047-x. [DOI] [PubMed] [Google Scholar]
  6. Cho C. H., Ogle C. W., Wong S. H., Koo M. W. Effects of zinc sulphate on ethanol- and indomethacin-induced ulceration and changes in prostaglandin E2 and histamine levels in the rat gastric glandular mucosa. Digestion. 1985;32(4):288–295. doi: 10.1159/000199250. [DOI] [PubMed] [Google Scholar]
  7. Corne S. J., Morrissey S. M., Woods R. J. Proceedings: A method for the quantitative estimation of gastric barrier mucus. J Physiol. 1974 Oct;242(2):116P–117P. [PubMed] [Google Scholar]
  8. Das K. M., Dubin R. Clinical pharmacokinetics of sulphasalazine. Clin Pharmacokinet. 1976 Nov-Dec;1(6):406–425. doi: 10.2165/00003088-197601060-00002. [DOI] [PubMed] [Google Scholar]
  9. Gould S. R. Letter: Prostaglandins, ulcerative colitis, and sulphasalazine. Lancet. 1975 Nov 15;2(7942):988–988. doi: 10.1016/s0140-6736(75)90414-6. [DOI] [PubMed] [Google Scholar]
  10. Guth P. H., Paulsen G., Nagata H. Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rat: effect of prostaglandin cytoprotection. Gastroenterology. 1984 Nov;87(5):1083–1090. [PubMed] [Google Scholar]
  11. Hoult J. R., Moore P. K. Effects of sulphasalazine and its metabolites on prostaglandin synthesis, inactivation and actions on smooth muscle. Br J Pharmacol. 1980 Apr;68(4):719–730. doi: 10.1111/j.1476-5381.1980.tb10865.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Konturek S. J., Piastucki I., Brzozowski T., Radecki T., Dembińska-Kieć A., Zmuda A., Gryglewski R. Role of prostaglandins in the formation of aspirin-induced gastric ulcers. Gastroenterology. 1981 Jan;80(1):4–9. [PubMed] [Google Scholar]
  13. Konturek S. J., Radecki T., Piastucki I., Brzozowski T., Drozdowicz D. Gastrocytoprotection by colloidal bismuth subcitrate (De-Nol) and sucralfate. Role of endogenous prostaglandins. Gut. 1987 Feb;28(2):201–205. doi: 10.1136/gut.28.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koo M. W., Cho C. H., Ogle C. W. Effects of cold-restraint stress on gastric ulceration and motility in rats. Pharmacol Biochem Behav. 1986 Oct;25(4):775–779. doi: 10.1016/0091-3057(86)90386-2. [DOI] [PubMed] [Google Scholar]
  15. Koo M. W., Ogle C. W., Cho C. H. The effect of cold-restraint stress on gastric emptying in rats. Pharmacol Biochem Behav. 1985 Dec;23(6):969–972. doi: 10.1016/0091-3057(85)90102-9. [DOI] [PubMed] [Google Scholar]
  16. Ogle C. W., Cho C. H., Dai S. Sulphasalazine and experimental stress ulcers. Agents Actions. 1985 Dec;17(2):153–157. doi: 10.1007/BF01966585. [DOI] [PubMed] [Google Scholar]
  17. Ogle C. W., Cho C. H. Effects of sulphasalazine on stress ulceration and mast cell degranulation in rat stomach. Eur J Pharmacol. 1985 Jun 7;112(2):285–286. doi: 10.1016/0014-2999(85)90512-6. [DOI] [PubMed] [Google Scholar]
  18. Peskar B. M., Lange K., Hoppe U., Peskar B. A. Ethanol stimulates formation of leukotriene C4 in rat gastric mucosa. Prostaglandins. 1986 Feb;31(2):283–293. doi: 10.1016/0090-6980(86)90054-7. [DOI] [PubMed] [Google Scholar]
  19. Rainsford K. D., Whitehouse M. W. Gastric mucus effusion elicited by oral copper compounds: potential anti-ulcer activity. Experientia. 1976 Sep 15;32(9):1172–1173. doi: 10.1007/BF01927608. [DOI] [PubMed] [Google Scholar]
  20. Robert A., Nezamis J. E., Lancaster C., Davis J. P., Field S. O., Hanchar A. J. Mild irritants prevent gastric necrosis through "adaptive cytoprotection" mediated by prostaglandins. Am J Physiol. 1983 Jul;245(1):G113–G121. doi: 10.1152/ajpgi.1983.245.1.G113. [DOI] [PubMed] [Google Scholar]
  21. Robert A., Nezamis J. E., Lancaster C., Hanchar A. J. Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology. 1979 Sep;77(3):433–443. [PubMed] [Google Scholar]
  22. Sircar J. C., Schwender C. F., Carethers M. E. Inhibition of soybean lipoxygenase by sulfasalazine and 5-aminosalicylic acid: a possible mode of action in ulcerative colitis. Biochem Pharmacol. 1983 Jan 1;32(1):170–172. doi: 10.1016/0006-2952(83)90673-1. [DOI] [PubMed] [Google Scholar]
  23. Szabo S., Trier J. S., Brown A., Schnoor J. Early vascular injury and increased vascular permeability in gastric mucosal injury caused by ethanol in the rat. Gastroenterology. 1985 Jan;88(1 Pt 2):228–236. doi: 10.1016/s0016-5085(85)80176-1. [DOI] [PubMed] [Google Scholar]
  24. Whittle B. J., Oren-Wolman N., Guth P. H. Gastric vasoconstrictor actions of leukotriene C4, PGF2 alpha, and thromboxane mimetic U-46619 on rat submucosal microcirculation in vivo. Am J Physiol. 1985 May;248(5 Pt 1):G580–G586. doi: 10.1152/ajpgi.1985.248.5.G580. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES