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. 1988 Apr;93(4):884–892. doi: 10.1111/j.1476-5381.1988.tb11476.x

The inhibition of sodium influx attenuates airway response to a specific antigen challenge.

M Souhrada 1, M H Souhrada 1, J F Souhrada 1
PMCID: PMC1853902  PMID: 3390656

Abstract

1. We have previously observed that manipulation of Na+ availability during passive in vitro sensitization altered electrophysiological and contractile changes of airway smooth muscle cells. The purpose of this study was to establish whether interference with Na+ influx during sensitization also influences the response of airway smooth muscle, both in vivo and in vitro, to a specific antigen challenge. 2. Isolated segments of trachea which had been sensitized to ovalbumin in the presence of the Na+ channel-blocking agent amiloride (10(-5) M) showed no electrical or contractile response to ovalbumin in spite of their ability to respond to histamine (10(-5) M). 3. Airway smooth muscle preparations sensitized to ovalbumin in a Na+-deficient medium failed to show any contractile response after exposure to ovalbumin and only a small depolarization of airway smooth muscle cells was detected. 4. Guinea-pigs were passively sensitized in vivo either in the absence of, or following pretreatment with, amiloride (1 mg kg-1 s.c.). These animals were then exposed to an ovalbumin inhalation challenge and both lung resistance (RL) and dynamic lung compliance (Cdyn) were measured. 5. After an inhalation challenge of sensitized animals, we observed a significant increase in lung resistance (RL) achieving a maximum of 489% of the baseline values and a decrease in dynamic lung compliance (Cdyn). Twenty min after ovalbumin challenge Cdyn was equivalent to 20% of baseline values. 6. In animals pretreated with amiloride during sensitization, the inhalation challenge caused only a small increase in RL achieving a maximum increase of 148% of baseline values, and a small decrease in Cdyn.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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