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. 1988 Jul;94(3):823–833. doi: 10.1111/j.1476-5381.1988.tb11593.x

Release of [3H]-noradrenaline from the sympathetic nerves to bovine mesenteric lymphatic vessels and its modification by alpha-agonists and antagonists.

J M Allen 1, J G McCarron 1, N G McHale 1, K D Thornbury 1
PMCID: PMC1854041  PMID: 2902893

Abstract

1. Isolated segments of bovine mesenteric lymphatic vessels were loaded with [3H]-noradrenaline and its efflux in response to field stimulation examined. Vessels were attached to an isometric force transducer for the simultaneous recording of mechanical activity. 2. Field stimulation at 1, 4 and 8 Hz (0.3 ms pulses, 1 min train) increased spontaneous contraction rate and evoked 3H release up to a maximum of 4.5% of total tissue 3H at 8 Hz. Output per pulse was maximal at 4 Hz. 3. Tetrodotoxin (3 x 10(-6) M) blocked the release of 3H in response to field stimulation although the drug did not attenuate release evoked by high K+ (65 mM) solution. Field-evoked release of 3H was also absent in Ca2+ -free solution containing EGTA (1 mM). 4. When vessels were preincubated with labelled transmitter plus cocaine (5 x 10(-5) M) evoked release of 3H was absent. After preloading with [3H]-noradrenaline, cocaine (10(-6) M) potentiated both the mechanical response to field stimulation and evoked 3H release. 5. The relatively non selective alpha-adrenoceptor antagonist phentolamine (3 x 10(-6) M) and the alpha 2-antagonists yohimbine (10(-8) M) and rauwolscine (10(-6) M) significantly increased evoked 3H release at both of the frequencies examined (1 and 4 Hz). In contrast, the selective alpha 1-antagonist prazosin (10(-6) M) failed to alter 3H release to 4 Hz stimulation although release at 1 Hz was potentiated in the presence of the drug. 6. The postsynaptic excitatory response to field stimulation remained in the presence of prazosin (10(-6) M), but was converted to an inhibitory effect in the presence of phentolamine (3 x 10(-6) M), yohimbine (10(-6) M) or rauwolscine (10(-6) M). 7. Evoked 3H efflux was significantly reduced by clonidine (10(-6) M), xylazine (10(-6) M) and exogenous noradrenaline (5 x 10(-7) M), although phenylephrine (10(-6) M) reduced release only at the lower of the two frequencies tested (1 Hz). 8. These findings suggest that release of 3H by field stimulation reflects endogenous transmitter release and that this is subject to autoinhibition via feedback onto inhibitory prejunctional alpha 2-adrenoceptors. The postjunctional excitatory response is mediated via postjunctional alpha 2-adrenoceptors.

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Selected References

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  1. Alberts P., Bartfai T., Stjärne L. Site(s) and ionic basis of alpha-autoinhibition and facilitation of "3H'noradrenaline secretion in guinea-pig vas deferens. J Physiol. 1981 Mar;312:297–334. doi: 10.1113/jphysiol.1981.sp013630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alessandrini C., Gerli R., Sacchi G., Ibba L., Pucci A. M., Fruschelli C. Cholinergic and adrenergic innervation of mesenterial lymph vessels in guinea pig. Lymphology. 1981 Mar;14(1):1–6. [PubMed] [Google Scholar]
  3. Allen J. M., McHale N. G. Neuromuscular transmission in bovine mesenteric lymphatics. Microvasc Res. 1986 Jan;31(1):77–83. doi: 10.1016/0026-2862(86)90008-7. [DOI] [PubMed] [Google Scholar]
  4. Allen J. M., McHale N. G., Rooney B. M. Effect of norepinephrine on contractility of isolated mesenteric lymphatics. Am J Physiol. 1983 Apr;244(4):H479–H486. doi: 10.1152/ajpheart.1983.244.4.H479. [DOI] [PubMed] [Google Scholar]
  5. BURNSTOCK G., HOLMAN M. E., KURIYAMA H. FACILITATION OF TRANSMISSION FROM AUTONOMIC NERVE TO SMOOTH MUSCLE OF GUINEA-PIG VAS DEFERENS. J Physiol. 1964 Jul;172:31–49. doi: 10.1113/jphysiol.1964.sp007401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Baker D. J., Drew G. M., Hilditch A. Presynaptic alpha-adrenoceptors: do exogenous and neuronally released noradrenaline act at different sites? Br J Pharmacol. 1984 Mar;81(3):457–464. doi: 10.1111/j.1476-5381.1984.tb10098.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bonaccorsi A., Hermsmeyer K., Smith C. B., Bohr D. F. Norepinephrine release in isolated arteries induced by K-free solution. Am J Physiol. 1977 Feb;232(2):H140–H145. doi: 10.1152/ajpheart.1977.232.2.H140. [DOI] [PubMed] [Google Scholar]
  8. Campbell T., Heath T. Intrinsic contractility of lymphatics in sheep and in dogs. Q J Exp Physiol Cogn Med Sci. 1973 Jul;58(3):207–217. doi: 10.1113/expphysiol.1973.sp002209. [DOI] [PubMed] [Google Scholar]
  9. Davies B. N., Withrington P. G. The release of noradrenaline by the sympathetic post-ganglionic nerves to the spleen of the cat in response to low frequency stimulation. Arch Int Pharmacodyn Ther. 1968 Jan;171(1):185–197. [PubMed] [Google Scholar]
  10. Docherty J. R. An investigation of presynaptic alpha-adrenoceptor subtypes in the pithed rat heart. Br J Pharmacol. 1983 Apr;78(4):655–657. doi: 10.1111/j.1476-5381.1983.tb09416.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Docherty J. R., Hyland L. Evidence for neuro-effector transmission through postjunctional alpha 2-adrenoceptors in human saphenous vein. Br J Pharmacol. 1985 Feb;84(2):573–576. doi: 10.1111/j.1476-5381.1985.tb12942.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Docherty J. R., Hyland L. No evidence for differences between pre- and post-junctional alpha 2-adrenoceptors. Br J Pharmacol. 1985 Oct;86(2):335–339. doi: 10.1111/j.1476-5381.1985.tb08901.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Docherty J. R., McGrath J. C. A comparison of pre- and post-junctional potencies of several alpha-adrenoceptor agonists in the cardiovascular system and anococcygeus muscle of the rat. Evidence for two types of post-junctional alpha-adrenoceptor. Naunyn Schmiedebergs Arch Pharmacol. 1980 Jun;312(2):107–116. doi: 10.1007/BF00569718. [DOI] [PubMed] [Google Scholar]
  14. Drew G. M., Whiting S. B. Evidence for two distinct types of postsynaptic alpha-adrenoceptor in vascular smooth muscle in vivo. Br J Pharmacol. 1979 Oct;67(2):207–215. doi: 10.1111/j.1476-5381.1979.tb08668.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Haefely W., Hürlimann A., Thoenen H. Relation between the rate of stimulation and the quantity of noradrenaline liberated from sympathetic nerve endings in the isolated perfused spleen of the cat. J Physiol. 1965 Nov;181(1):48–58. doi: 10.1113/jphysiol.1965.sp007744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hughes J., Roth R. H. Variation in noradrenaline output with changes in stimulus frequency and train length: role of different noradrenaline pools. Br J Pharmacol. 1974 Jul;51(3):373–381. doi: 10.1111/j.1476-5381.1974.tb10672.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kalsner S. Clonidine and presynaptic adrenoceptor theory. Br J Pharmacol. 1985 May;85(1):143–147. doi: 10.1111/j.1476-5381.1985.tb08841.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kirpekar S. M., Misu Y. Release of noradrenaline by splenic nerve stimulation and its dependence on calcium. J Physiol. 1967 Jan;188(2):219–234. doi: 10.1113/jphysiol.1967.sp008135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kirpekar S. M., Wakade A. R. Release of noradrenaline from the cat spleen by potassium. J Physiol. 1968 Feb;194(3):595–608. doi: 10.1113/jphysiol.1968.sp008427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kobinger W., Pichler L. Presynaptic activity of the imidazolidine derivative ST 587, a highly selective alpha 1-adrenoceptor agonist. Eur J Pharmacol. 1982 Aug 27;82(3-4):203–206. doi: 10.1016/0014-2999(82)90514-3. [DOI] [PubMed] [Google Scholar]
  21. Langer S. Z. Presynaptic regulation of the release of catecholamines. Pharmacol Rev. 1980 Dec;32(4):337–362. [PubMed] [Google Scholar]
  22. Langer S. Z. Selective metabolic pathways for noradrena-line in the peripheral and in the central nervous system. Med Biol. 1974 Dec;52(6):372–383. [PubMed] [Google Scholar]
  23. McGeown J. G., McHale N. G., Thornbury K. D. The role of external compression and movement in lymph propulsion in the sheep hind limb. J Physiol. 1987 Jun;387:83–93. doi: 10.1113/jphysiol.1987.sp016564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. McHale N. G., Allen J. M., McCarron J. G. Transient excitatory responses to sustained stimulation of intramural nerves in isolated bovine lymphatic vessels. Q J Exp Physiol. 1988 Mar;73(2):175–182. doi: 10.1113/expphysiol.1988.sp003130. [DOI] [PubMed] [Google Scholar]
  25. McHale N. G., Roddie I. C. The effect of transmural pressure on pumping activity in isolated bovine lymphatic vessels. J Physiol. 1976 Oct;261(2):255–269. doi: 10.1113/jphysiol.1976.sp011557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. McHale N. G., Roddie I. C., Thornbury K. D. Nervous modulation of spontaneous contractions in bovine mesenteric lymphatics. J Physiol. 1980 Dec;309:461–472. doi: 10.1113/jphysiol.1980.sp013520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. NARAHASHI T., MOORE J. W., SCOTT W. R. TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS. J Gen Physiol. 1964 May;47:965–974. doi: 10.1085/jgp.47.5.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Starke K. Regulation of noradrenaline release by presynaptic receptor systems. Rev Physiol Biochem Pharmacol. 1977;77:1–124. doi: 10.1007/BFb0050157. [DOI] [PubMed] [Google Scholar]
  29. Stjärne L., Brundin J. Frequency-dependence of 3H-noradrenaline secretion from human vasoconstrictor nerves: modification by factors interfering with alpha-or beta-adrenoceptor or prostaglandin E2 mediated control. Acta Physiol Scand. 1977 Oct;101(2):199–210. doi: 10.1111/j.1748-1716.1977.tb05999.x. [DOI] [PubMed] [Google Scholar]
  30. Stjärne L. Comparison of secretion of sympathetic neurotransmitter induced by nerve stimulation with that evoked by high potassium, as triggers of dual alpha-adrenoceptor mediated negative feed-back control of noradrenaline secretion. Prostaglandins. 1973 Apr;3(4):421–426. doi: 10.1016/0090-6980(73)90149-4. [DOI] [PubMed] [Google Scholar]
  31. Su C., Bevan J. A. Blockade of the nicotine-induced norepinephrine release by cocaine, phenoxybenzamine and desipramine. J Pharmacol Exp Ther. 1970 Nov;175(2):533–540. [PubMed] [Google Scholar]
  32. Su C., Bevan J. A. The release of H3-norepinephrine in arterial strips studied by the technique of superfusion and transmural stimulation. J Pharmacol Exp Ther. 1970 Mar;172(1):62–68. [PubMed] [Google Scholar]
  33. Sullivan A. T., Drew G. M. Pharmacological characterisation of pre- and postsynaptic alpha-adrenoceptors in dog saphenous vein. Naunyn Schmiedebergs Arch Pharmacol. 1980 Nov;314(3):249–258. doi: 10.1007/BF00498546. [DOI] [PubMed] [Google Scholar]
  34. Westfall T. C. Local regulation of adrenergic neurotransmission. Physiol Rev. 1977 Oct;57(4):659–728. doi: 10.1152/physrev.1977.57.4.659. [DOI] [PubMed] [Google Scholar]

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