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. 1988 Dec;95(4):1262–1270. doi: 10.1111/j.1476-5381.1988.tb11763.x

Effects of neuropeptides and capsaicin on the canine tracheal vasculature in vivo.

R O Salonen 1, S E Webber 1, J G Widdicombe 1
PMCID: PMC1854247  PMID: 3219486

Abstract

1. The nonadrenergic, noncholinergic nervous system may control the airway vasculature via various neuropeptides. We have perfused the cranial tracheal arteries of the anaesthetized dog and investigated the effects of neuropeptides and capsaicin (which is supposed to release neuropeptides from sensory nerve endings) on the tracheal vasculature by injecting them locally into the perfusion system. 2. Neurokinin A (NKA, 0.02-20 pmol), calcitonin gene-related peptide (CGRP, 2-200 pmol) and peptide histidine isoleucine (PHI, 0.02-2 nmol) dose-dependently decreased tracheal vascular resistance (Rtv). NKA was 10 and 100 times more potent than CGRP and PHI, respectively. The duration of the response to CGRP was greatly prolonged with larger doses. Galanin (0.2-2 nmol) had no appreciable effect on Rtv. 3. Neuropeptide Y (NPY 0.02-2 nmol) and bombesin (0.02-10 nmol) dose-dependently increased Rtv. However, the dose-response curve for bombesin was bell-shaped suggesting the development of tachyphylaxis with larger doses. In smaller doses, bombesin was twice as potent as NPY. The duration of the response to NPY was prolonged with larger doses. 4. With the exception of PHI no neuropeptide altered tracheal smooth muscle tone; PHI (1 and 2 nmol) caused small dilatations of the trachea. 5. The effects of capsaicin (2-100 nmol) were complex. Usually, the vascular response had two dose-dependent phases: a rapid vasoconstriction followed by a small, longer-lasting vasodilatation. The tracheal smooth muscle response was usually biphasic, a contraction followed by a relaxation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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  1. Baier H., Long W. M., Wanner A. Bronchial circulation in asthma. Respiration. 1985;48(3):199–205. doi: 10.1159/000194830. [DOI] [PubMed] [Google Scholar]
  2. Barnes P. J., Dixon C. M. The effect of inhaled vasoactive intestinal peptide on bronchial reactivity to histamine in humans. Am Rev Respir Dis. 1984 Aug;130(2):162–166. doi: 10.1164/arrd.1984.130.2.162. [DOI] [PubMed] [Google Scholar]
  3. Barnes P. J. Neural control of human airways in health and disease. Am Rev Respir Dis. 1986 Dec;134(6):1289–1314. doi: 10.1164/arrd.1986.134.5.1289. [DOI] [PubMed] [Google Scholar]
  4. Barrowcliffe M. P., Morice A., Jones J. G., Sever P. S. Pulmonary clearance of vasoactive intestinal peptide. Thorax. 1986 Feb;41(2):88–93. doi: 10.1136/thx.41.2.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brain S. D., Williams T. J., Tippins J. R., Morris H. R., MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature. 1985 Jan 3;313(5997):54–56. doi: 10.1038/313054a0. [DOI] [PubMed] [Google Scholar]
  6. Buck S. H., Burks T. F. The neuropharmacology of capsaicin: review of some recent observations. Pharmacol Rev. 1986 Sep;38(3):179–226. [PubMed] [Google Scholar]
  7. Bundgaard A., Enehjelm S. D., Aggestrup S. Pretreatment of exercise-induced asthma with inhaled vasoactive intestinal peptide (VIP). Eur J Respir Dis Suppl. 1983;128(Pt 2):427–429. [PubMed] [Google Scholar]
  8. Clarke J. G., Davies G. J., Kerwin R., Hackett D., Larkin S., Dawbarn D., Lee Y., Bloom S. R., Yacoub M., Maseri A. Coronary artery infusion of neuropeptide Y in patients with angina pectoris. Lancet. 1987 May 9;1(8541):1057–1059. doi: 10.1016/s0140-6736(87)90483-1. [DOI] [PubMed] [Google Scholar]
  9. Corder R., Lowry P. J., Withrington P. G. The actions of the peptides, neuropeptide Y and peptide YY, on the vascular and capsular smooth muscle of the isolated, blood-perfused spleen of the dog. Br J Pharmacol. 1987 Apr;90(4):785–790. doi: 10.1111/j.1476-5381.1987.tb11232.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Deffebach M. E., Charan N. B., Lakshminarayan S., Butler J. The bronchial circulation. Small, but a vital attribute of the lung. Am Rev Respir Dis. 1987 Feb;135(2):463–481. doi: 10.1164/arrd.1987.135.2.463. [DOI] [PubMed] [Google Scholar]
  11. Ekblad E., Håkanson R., Sundler F., Wahlestedt C. Galanin: neuromodulatory and direct contractile effects on smooth muscle preparations. Br J Pharmacol. 1985 Sep;86(1):241–246. doi: 10.1111/j.1476-5381.1985.tb09455.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Erspamer V., Erspamer G. F., Inselvini M., Negri L. Occurrence of bombesin and alytesin in extracts of the skin of three European discoglossid frogs and pharmacological actions of bombesin on extravascular smooth muscle. Br J Pharmacol. 1972 Jun;45(2):333–348. doi: 10.1111/j.1476-5381.1972.tb08087.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ghatei M. A., Springall D. R., Richards I. M., Oostveen J. A., Griffin R. L., Cadieux A., Polak J. M., Bloom S. R. Regulatory peptides in the respiratory tract of Macaca fascicularis. Thorax. 1987 Jun;42(6):431–439. doi: 10.1136/thx.42.6.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaufman M. P., Ordway G. A., Longhurst J. C., Mitchell J. H. Reflex relaxation of tracheal smooth muscle by thin-fiber muscle afferents in dogs. Am J Physiol. 1982 Sep;243(3):R383–R388. doi: 10.1152/ajpregu.1982.243.3.R383. [DOI] [PubMed] [Google Scholar]
  15. Laitinen L. A., Laitinen M. A., Widdicombe J. G. Dose-related effects of pharmacological mediators on tracheal vascular resistance in dogs. Br J Pharmacol. 1987 Dec;92(4):703–709. doi: 10.1111/j.1476-5381.1987.tb11374.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laitinen L. A., Laitinen M. V., Widdicombe J. G. Parasympathetic nervous control of tracheal vascular resistance in the dog. J Physiol. 1987 Apr;385:135–146. doi: 10.1113/jphysiol.1987.sp016488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lundberg J. M., Tatemoto K. Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to alpha-adrenoceptor blockade. Acta Physiol Scand. 1982 Dec;116(4):393–402. doi: 10.1111/j.1748-1716.1982.tb07157.x. [DOI] [PubMed] [Google Scholar]
  18. Lung M. A., Phipps R. J., Wang J. C., Widdicombe J. G. Control of nasal vasculature and airflow resistance in the dog. J Physiol. 1984 Apr;349:535–551. doi: 10.1113/jphysiol.1984.sp015172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lung M. A., Wang J. C., Cheng K. K. Bronchial circulation: an auto-perfusion method for assessing its vasomotor activity and the study of alpha- and beta-adrenoceptors in the bronchial artery. Life Sci. 1976 Aug 15;19(4):577–580. doi: 10.1016/0024-3205(76)90239-3. [DOI] [PubMed] [Google Scholar]
  20. Martling C. R., Anggård A., Lundberg J. M. Non-cholinergic vasodilation in the tracheobronchial tree of the cat induced by vagal nerve stimulation. Acta Physiol Scand. 1985 Oct;125(2):343–346. doi: 10.1111/j.1748-1716.1985.tb07726.x. [DOI] [PubMed] [Google Scholar]
  21. Pernow J., Lundberg J. M., Kaijser L. Vasoconstrictor effects in vivo and plasma disappearance rate of neuropeptide Y in man. Life Sci. 1987 Jan 5;40(1):47–54. doi: 10.1016/0024-3205(87)90251-7. [DOI] [PubMed] [Google Scholar]
  22. Polak J. M., Bloom S. R. Occurrence and distribution of regulatory peptides in the respiratory tract. Recent Results Cancer Res. 1985;99:1–16. doi: 10.1007/978-3-642-82533-0_1. [DOI] [PubMed] [Google Scholar]
  23. Regoli D., Drapeau G., Dion S., D'Orléans-Juste P. Pharmacological receptors for substance P and neurokinins. Life Sci. 1987 Jan 12;40(2):109–117. doi: 10.1016/0024-3205(87)90349-3. [DOI] [PubMed] [Google Scholar]
  24. Ryan U. S. Metabolic activity of pulmonary endothelium: modulations of structure and function. Annu Rev Physiol. 1986;48:263–277. doi: 10.1146/annurev.ph.48.030186.001403. [DOI] [PubMed] [Google Scholar]
  25. Saria A., Theodorsson-Norheim E., Gamse R., Lundberg J. M. Release of substance P- and substance K-like immunoreactivities from the isolated perfused guinea-pig lung. Eur J Pharmacol. 1984 Oct 30;106(1):207–208. doi: 10.1016/0014-2999(84)90699-x. [DOI] [PubMed] [Google Scholar]
  26. Skofitsch G., Donnerer J., Petronijevic S., Saria A., Lembeck F. Release of histamine by neuropeptides from the perfused rat hindquarter. Naunyn Schmiedebergs Arch Pharmacol. 1983 Mar;322(2):153–157. doi: 10.1007/BF00512389. [DOI] [PubMed] [Google Scholar]
  27. Vanhoutte P. M., Rubanyi G. M., Miller V. M., Houston D. S. Modulation of vascular smooth muscle contraction by the endothelium. Annu Rev Physiol. 1986;48:307–320. doi: 10.1146/annurev.ph.48.030186.001515. [DOI] [PubMed] [Google Scholar]
  28. Zaidi M., Bevis P. J., Girgis S. I., Lynch C., Stevenson J. C., MacIntyre I. Circulating CGRP comes from the perivascular nerves. Eur J Pharmacol. 1985 Nov 5;117(2):283–284. doi: 10.1016/0014-2999(85)90616-8. [DOI] [PubMed] [Google Scholar]

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