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. 1989 Mar;96(3):637–644. doi: 10.1111/j.1476-5381.1989.tb11863.x

N-methylhydroxylamine inhibits and M&B 22948 potentiates relaxations of the mouse anococcygeus to non-adrenergic, non-cholinergic field stimulation and to nitrovasodilator drugs.

A Gibson 1, S Mirzazadeh 1
PMCID: PMC1854397  PMID: 2541847

Abstract

1. The effects of N-methylhydroxylamine (NMH) and of M&B 22948 on relaxations of the mouse anococcygeus to non-adrenergic, non-cholinergic (NANC) field stimulation and to a number of smooth muscle relaxant drugs were investigated. 2. Relaxations to NANC field stimulation (10 Hz; 60 s train) were reversibly blocked by NMH (1-5 mM), which also caused weak, transient reductions of carbachol (50 microM)-induced tone. N,N-dimethylhydroxylamine (2 mM) and hydroxylamine (5 microM) reduced tone to the same extent as NMH, but neither produced any inhibition of NANC relaxations. 3. M&B 22948 10 microM, which by itself reduced tone by 12%, potentiated submaximal but not maximal relaxations to NANC field stimulation; overall the log frequency-response curve was displaced to the left by a factor of 2. 4. Sodium nitroprusside (0.01-1 microM), hydroxylamine (0.5-100 microM), and nitric oxide (2-200 microM) all relaxed carbachol-induced tone; relaxations to submaximal concentrations of these nitrovasodilators were reduced in the presence of 2 mM NMH, and potentiated in the presence of 10 microM M&B 22948. 5. Neither NMH (2 mM) nor M&B 22948 (10 microM) affected relaxations induced by submaximal concentrations of vasoactive intestinal peptide (VIP; 1 microM), papaverine (10 microM), 3-isobutyl-1-methyl-xanthine (10 microM), or 8-bromo-cyclic guanosine monophosphate (100 microM); relaxations to adenosine 5'-triphosphate (ATP, 2 mM) were unaffected by M&B 22948, but were potentiated by NMH.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

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