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. 1989 Apr;96(4):993–999. doi: 10.1111/j.1476-5381.1989.tb11912.x

The distribution of gamma-adrenoceptors and P2 purinoceptors in mesenteric arteries and veins of the guinea-pig.

G D Hirst 1, P Jobling 1
PMCID: PMC1854435  PMID: 2743089

Abstract

1. Membrane potential changes and contractions were recorded from mesenteric arteries and veins of the guinea-pig, during perivascular nerve stimulation or application of noradrenaline or adenosine triphosphate (ATP). 2. After alpha-adrenoceptor blockade, noradrenaline activated low affinity adrenoceptors (gamma-adrenoceptors) causing depolarization and arterial contraction only in the presence of an inhibitor of catecholamine uptake. 3. Noradrenaline did not cause depolarization or contraction of the vein after alpha-adrenoceptor blockade even after catecholamine uptake was blocked. 4. Adenosine triphosphate caused depolarization and contraction of both arteries and veins. These responses were abolished by alpha-,beta-,methylene adenosine triphosphate (Me-ATP). 5. Me-ATP abolished rapid excitatory junction potentials (e.j.ps) caused by perivascular nerve stimulation of arteries but had no effect on arterial responses mediated by gamma-adrenoceptors. 6. In veins, perivascular nerve stimulation evoked slow e.j.ps which persisted in the presence of Me-ATP but were abolished after blockade of alpha-adrenoceptors. 7. The observations indicate that P2 purinoceptors are present on both mesenteric artery and vein whilst gamma-adrenoceptors are localized near the neuromuscular junction of the artery. However gamma-adrenoceptors do not appear to be directly involved in the generation of arterial e.j.ps.

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Selected References

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  1. Burnstock G., Warland J. J. A pharmacological study of the rabbit saphenous artery in vitro: a vessel with a large purinergic contractile response to sympathetic nerve stimulation. Br J Pharmacol. 1987 Jan;90(1):111–120. doi: 10.1111/j.1476-5381.1987.tb16830.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Byrne N. G., Hirst G. D., Large W. A. Electrophysiological analysis of the nature of adrenoceptors in the rat basilar artery during development. Br J Pharmacol. 1985 Sep;86(1):217–227. doi: 10.1111/j.1476-5381.1985.tb09452.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Byrne N. G., Large W. A. The effect of alpha, beta-methylene ATP on the depolarization evoked by noradrenaline (gamma-adrenoceptor response) and ATP in the immature rat basilar artery. Br J Pharmacol. 1986 May;88(1):6–8. doi: 10.1111/j.1476-5381.1986.tb09464.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cheung D. W. An electrophysiological study of alpha-adrenoceptor mediated excitation-contraction coupling in the smooth muscle cells of the rat saphenous vein. Br J Pharmacol. 1985 Jan;84(1):265–271. [PMC free article] [PubMed] [Google Scholar]
  5. Cheung D. W. Two components in the cellular response of rat tail arteries to nerve stimulation. J Physiol. 1982 Jul;328:461–468. doi: 10.1113/jphysiol.1982.sp014277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fujiwara S., Itoh T., Suzuki H. Membrane properties and excitatory neuromuscular transmission in the smooth muscle of dog cerebral arteries. Br J Pharmacol. 1982 Oct;77(2):197–208. doi: 10.1111/j.1476-5381.1982.tb09286.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furness J. B. The adrenergic innervation of the vessels supplying and draining the gastrointestinal tract. Z Zellforsch Mikrosk Anat. 1971;113(1):67–82. doi: 10.1007/BF00331202. [DOI] [PubMed] [Google Scholar]
  8. Hill C. E., Hirst G. D., Silverberg G. D., van Helden D. F. Sympathetic innervation and excitability of arterioles originating from the rat middle cerebral artery. J Physiol. 1986 Feb;371:305–316. doi: 10.1113/jphysiol.1986.sp015976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirst G. D., Lew M. J. Lack of involvement of alpha-adrenoceptors in sympathetic neural vasoconstriction in the hindquarters of the rabbit. Br J Pharmacol. 1987 Jan;90(1):51–60. doi: 10.1111/j.1476-5381.1987.tb16824.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirst G. D., Neild T. O. Evidence for two populations of excitatory receptors for noradrenaline on arteriolar smooth muscle. Nature. 1980 Feb 21;283(5749):767–768. doi: 10.1038/283767a0. [DOI] [PubMed] [Google Scholar]
  11. Hirst G. D., Neild T. O. Localization of specialized noradrenaline receptors at neuromuscular junctions on arterioles of the guinea-pig. J Physiol. 1981;313:343–350. doi: 10.1113/jphysiol.1981.sp013669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hirst G. D., Neild T. O., Silverberg G. D. Noradrenaline receptors on the rat basilar artery. J Physiol. 1982 Jul;328:351–360. doi: 10.1113/jphysiol.1982.sp014268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hirst G. D. Neuromuscular transmission in arterioles of guinea-pig submucosa. J Physiol. 1977 Dec;273(1):263–275. doi: 10.1113/jphysiol.1977.sp012093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holman M. E., Surprenant A. M. Some properties of the excitatory junction potentials recorded from saphenous arteries of rabbits. J Physiol. 1979 Feb;287:337–351. doi: 10.1113/jphysiol.1979.sp012663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Holman M. E., Surprenant A. An electrophysiological analysis of the effects of noradrenaline and alpha-receptor antagonists on neuromuscular transmission in mammalian muscular arteries. Br J Pharmacol. 1980;71(2):651–661. doi: 10.1111/j.1476-5381.1980.tb10986.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ishikawa S. Actions of ATP and alpha, beta-methylene ATP on neuromuscular transmission and smooth muscle membrane of the rabbit and guinea-pig mesenteric arteries. Br J Pharmacol. 1985 Dec;86(4):777–787. doi: 10.1111/j.1476-5381.1985.tb11099.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kreulen D. L. Activation of mesenteric arteries and veins by preganglionic and postganglionic nerves. Am J Physiol. 1986 Dec;251(6 Pt 2):H1267–H1275. doi: 10.1152/ajpheart.1986.251.6.H1267. [DOI] [PubMed] [Google Scholar]
  18. Kuriyama H., Suyama A. Multiple actions of cocaine on neuromuscular transmission and smooth muscle cells of the guinea-pig mesenteric artery. J Physiol. 1983 Apr;337:631–654. doi: 10.1113/jphysiol.1983.sp014646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kuriyama H., Suzuki H. Adrenergic transmissions in the guinea-pig mesenteric artery and their cholinergic modulations. J Physiol. 1981 Aug;317:383–396. doi: 10.1113/jphysiol.1981.sp013831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Laher I., Khayal M. A., Bevan J. A. Norepinephrine-sensitive, phenoxybenzamine-resistant receptor sites associated with contraction in rabbit arterial but not venous smooth muscle: possible role in adrenergic neurotransmission. J Pharmacol Exp Ther. 1986 May;237(2):364–368. [PubMed] [Google Scholar]
  21. Lightman S. L., Iversen L. L. The role of uptake2 in the extraneuronal metabolism of catecholamines in the isolated rat heart. Br J Pharmacol. 1969 Nov;37(3):638–649. doi: 10.1111/j.1476-5381.1969.tb08502.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Luff S. E., McLachlan E. M., Hirst G. D. An ultrastructural analysis of the sympathetic neuromuscular junctions on arterioles of the submucosa of the guinea pig ileum. J Comp Neurol. 1987 Mar 22;257(4):578–594. doi: 10.1002/cne.902570407. [DOI] [PubMed] [Google Scholar]
  23. Melchiorre C., Yong M. S., Benfey B. G., Belleau B. Molecular properties of the adrenergic alpha receptor. 2. Optimum covalent inhibition by two different prototypes of polyamine disulfides. J Med Chem. 1978 Nov;21(11):1126–1132. doi: 10.1021/jm00209a007. [DOI] [PubMed] [Google Scholar]
  24. Muramatsu I. Evidence for sympathetic, purinergic transmission in the mesenteric artery of the dog. Br J Pharmacol. 1986 Mar;87(3):478–480. doi: 10.1111/j.1476-5381.1986.tb10187.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sneddon P., Burnstock G. ATP as a co-transmitter in rat tail artery. Eur J Pharmacol. 1984 Oct 30;106(1):149–152. doi: 10.1016/0014-2999(84)90688-5. [DOI] [PubMed] [Google Scholar]
  26. Surprenant A. A comparative study of neuromuscular transmission in several mammalian muscular arteries. Pflugers Arch. 1980 Jul;386(1):85–91. doi: 10.1007/BF00584192. [DOI] [PubMed] [Google Scholar]
  27. Suzuki H. Adrenergic transmission in the dog mesenteric vein and its modulation by alpha-adrenoceptor antagonists. Br J Pharmacol. 1984 Mar;81(3):479–489. doi: 10.1111/j.1476-5381.1984.tb10101.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Suzuki H. Effects of endogenous and exogenous noradrenaline on the smooth muscle of guinea-pig mesenteric vein. J Physiol. 1981 Dec;321:495–512. doi: 10.1113/jphysiol.1981.sp013999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Suzuki H., Kou K. Electrical components contributing to the nerve-mediated contractions in the smooth muscles of the rabbit ear artery. Jpn J Physiol. 1983;33(5):743–756. doi: 10.2170/jjphysiol.33.743. [DOI] [PubMed] [Google Scholar]
  30. Van Helden D. F. Electrophysiology of neuromuscular transmission in guinea-pig mesenteric veins. J Physiol. 1988 Jul;401:469–488. doi: 10.1113/jphysiol.1988.sp017173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. von Kügelgen I., Starke K. Noradrenaline and adenosine triphosphate as co-transmitters of neurogenic vasoconstriction in rabbit mesenteric artery. J Physiol. 1985 Oct;367:435–455. doi: 10.1113/jphysiol.1985.sp015834. [DOI] [PMC free article] [PubMed] [Google Scholar]

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