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British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1989 Apr;96(4):817–822. doi: 10.1111/j.1476-5381.1989.tb11889.x

Evidence that the hypertrophic action of clenbuterol on denervated rat muscle is not propranolol-sensitive.

C A Maltin 1, S M Hay 1, M I Delday 1, P J Reeds 1, R M Palmer 1
PMCID: PMC1854445  PMID: 2472845

Abstract

1. The effect of propranolol on the clenbuterol-induced protein anabolism in innervated and denervated soleus and plantaris muscles of the rat was studied. 2. The response to the beta-agonist, clenbuterol, in both innervated and denervated muscles, was not significantly inhibited by the beta-antagonist, propranolol. 3. The results provide further evidence to suggest that the action of clenbuterol on skeletal muscle protein accretion may not be directly mediated by beta-adrenoceptors.

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Selected References

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  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowman W. C., Nott M. W. Actions of sympathomimetic amines and their antagonists on skeletal muscle. Pharmacol Rev. 1969 Mar;21(1):27–72. [PubMed] [Google Scholar]
  3. Emery P. W., Rothwell N. J., Stock M. J., Winter P. D. Chronic effects of beta 2-adrenergic agonists on body composition and protein synthesis in the rat. Biosci Rep. 1984 Jan;4(1):83–91. doi: 10.1007/BF01120827. [DOI] [PubMed] [Google Scholar]
  4. Festoff B. W., Oliver K. L., Reddy N. B. In vitro studies of skeletal muscle membranes. Adenylate cyclase of fast and slow twitch muscle and the effects of denervation. J Membr Biol. 1977 Apr 22;32(3-4):331–343. doi: 10.1007/BF01905226. [DOI] [PubMed] [Google Scholar]
  5. Garber A. J., Karl I. E., Kipnis D. M. Alanine and glutamine synthesis and release from skeletal muscle. IV. beta-Adrenergic inhibition of amino acid release. J Biol Chem. 1976 Feb 10;251(3):851–857. [PubMed] [Google Scholar]
  6. Hayashi M., Freiman D. G. An improved method of fixation for formalin-sensitive enzymes with special reference to myosin adenosine triphosphatase. J Histochem Cytochem. 1966 Aug;14(8):577–581. doi: 10.1177/14.8.577. [DOI] [PubMed] [Google Scholar]
  7. Maillet C. M., Garber A. J. Adrenergic and serotonergic regulation of skeletal muscle metabolism in the rat: specificity of the serotonin- and isoproterenol-stimulable adenylyl cyclase in sarcolemma. Mol Cell Endocrinol. 1986 Dec;48(2-3):179–190. doi: 10.1016/0303-7207(86)90040-7. [DOI] [PubMed] [Google Scholar]
  8. Maisel A. S., Motulsky H. J., Insel P. A. Propranolol treatment externalizes beta-adrenergic receptors in guinea pig myocardium and prevents further externalization by ischemia. Circ Res. 1987 Jan;60(1):108–112. doi: 10.1161/01.res.60.1.108. [DOI] [PubMed] [Google Scholar]
  9. Maltin C. A., Delday M. I., Hay S. M., Smith F. G., Lobley G. E., Reeds P. J. The effect of the anabolic agent, clenbuterol, on overloaded rat skeletal muscle. Biosci Rep. 1987 Feb;7(2):143–149. doi: 10.1007/BF01121878. [DOI] [PubMed] [Google Scholar]
  10. Maltin C. A., Delday M. I., Hay S. M., Smith F. G., Reeds P. J. Propranolol apparently separates the physical and compositional characteristics of muscle growth induced by clenbuterol. Biosci Rep. 1987 Jan;7(1):51–57. doi: 10.1007/BF01122727. [DOI] [PubMed] [Google Scholar]
  11. Maltin C. A., Delday M. I., Reeds P. J. The effect of a growth promoting drug, clenbuterol, on fibre frequency and area in hind limb muscles from young male rats. Biosci Rep. 1986 Mar;6(3):293–299. doi: 10.1007/BF01115158. [DOI] [PubMed] [Google Scholar]
  12. Maltin C. A., Hay S. M., Delday M. I., Smith F. G., Lobley G. E., Reeds P. J. Clenbuterol, a beta agonist, induces growth in innervated and denervated rat soleus muscle via apparently different mechanisms. Biosci Rep. 1987 Jun;7(6):525–532. doi: 10.1007/BF01116510. [DOI] [PubMed] [Google Scholar]
  13. Maltin C. A., Reeds P. J., Delday M. I., Hay S. M., Smith F. G., Lobley G. E. Inhibition and reversal of denervation-induced atrophy by the beta-agonist growth promoter, clenbuterol. Biosci Rep. 1986 Sep;6(9):811–818. doi: 10.1007/BF01117104. [DOI] [PubMed] [Google Scholar]
  14. Pullar J. D., Webster A. J. The energy cost of fat and protein deposition in the rat. Br J Nutr. 1977 May;37(3):355–363. doi: 10.1079/bjn19770039. [DOI] [PubMed] [Google Scholar]
  15. Reeds P. J., Hay S. M., Dorward P. M., Palmer R. M. The effect of beta-agonists and antagonists on muscle growth and body composition of young rats (Rattus sp.). Comp Biochem Physiol C. 1988;89(2):337–341. doi: 10.1016/0742-8413(88)90234-4. [DOI] [PubMed] [Google Scholar]
  16. Reeds P. J., Hay S. M., Dorwood P. M., Palmer R. M. Stimulation of muscle growth by clenbuterol: lack of effect on muscle protein biosynthesis. Br J Nutr. 1986 Jul;56(1):249–258. doi: 10.1079/bjn19860104. [DOI] [PubMed] [Google Scholar]
  17. Rothwell N. J., Stock M. J., Sudera D. K. Changes in tissue blood flow and beta-receptor density of skeletal muscle in rats treated with the beta2-adrenoceptor agonist clenbuterol. Br J Pharmacol. 1987 Mar;90(3):601–607. doi: 10.1111/j.1476-5381.1987.tb11211.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schmalbruch H. Satellite cells of rat muscles as studied by freeze-fracturing. Anat Rec. 1978 Jul;191(3):371–376. doi: 10.1002/ar.1091910309. [DOI] [PubMed] [Google Scholar]
  19. Shand D. G. Pharmacokinetic properties of the beta-adrenergic receptor blocking drugs. Drugs. 1974;7(1):39–47. doi: 10.2165/00003495-197407010-00003. [DOI] [PubMed] [Google Scholar]
  20. Williams R. S., Caron M. G., Daniel K. Skeletal muscle beta-adrenergic receptors: variations due to fiber type and training. Am J Physiol. 1984 Feb;246(2 Pt 1):E160–E167. doi: 10.1152/ajpendo.1984.246.2.E160. [DOI] [PubMed] [Google Scholar]

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