Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1989 Jul;97(3):968–972. doi: 10.1111/j.1476-5381.1989.tb12038.x

Endothelin- and neuropeptide Y-induced vasoconstriction of human epicardial coronary arteries in vitro.

A Franco-Cereceda 1
PMCID: PMC1854593  PMID: 2667681

Abstract

1. The effects of the recently discovered peptide endothelin and neuropeptide Y (NPY) on human epicardial coronary arteries were studied in vitro. 2. Endothelin induced a concentration-dependent, endothelium-independent, long-lasting vasoconstriction regardless of vessel size. NPY evoked contractions of small coronary arteries with a similar potency to that of endothelin, although to a significantly lower degree. Large coronary arteries did not respond to NPY. Endothelin did not relax coronary arteries precontracted with potassium. 3. The effect of endothelin was dependent on extracellular Ca2+ and, like NPY, significantly reduced by the Ca2+-antagonist nifiedipine. 4. In conclusion, endothelin is a potent human vasoconstrictor in vitro. It is suggested that endothelin may be involved in the regulation of coronary blood flow.

Full text

PDF
968

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auguet M., Delaflotte S., Chabrier P. E., Pirotzky E., Clostre F., Braquet P. Endothelin and Ca++ agonist Bay K 8644: different vasoconstrictive properties. Biochem Biophys Res Commun. 1988 Oct 14;156(1):186–192. doi: 10.1016/s0006-291x(88)80822-2. [DOI] [PubMed] [Google Scholar]
  2. Clarke J. G., Davies G. J., Kerwin R., Hackett D., Larkin S., Dawbarn D., Lee Y., Bloom S. R., Yacoub M., Maseri A. Coronary artery infusion of neuropeptide Y in patients with angina pectoris. Lancet. 1987 May 9;1(8541):1057–1059. doi: 10.1016/s0140-6736(87)90483-1. [DOI] [PubMed] [Google Scholar]
  3. Franco-Cereceda A., Lundberg J. M., Dahlöf C. Neuropeptide Y and sympathetic control of heart contractility and coronary vascular tone. Acta Physiol Scand. 1985 Jul;124(3):361–369. doi: 10.1111/j.1748-1716.1985.tb07671.x. [DOI] [PubMed] [Google Scholar]
  4. Franco-Cereceda A., Lundberg J. M. Potent effects of neuropeptide Y and calcitonin gene-related peptide on human coronary vascular tone in vitro. Acta Physiol Scand. 1987 Sep;131(1):159–160. doi: 10.1111/j.1748-1716.1987.tb08219.x. [DOI] [PubMed] [Google Scholar]
  5. Franco-Cereceda A., Rudehill A., Lundberg J. M. Calcitonin gene-related peptide but not substance P mimics capsaicin-induced coronary vasodilation in the pig. Eur J Pharmacol. 1987 Oct 13;142(2):235–243. doi: 10.1016/0014-2999(87)90112-9. [DOI] [PubMed] [Google Scholar]
  6. Furchgott R. F. The role of endothelium in the responses of vascular smooth muscle to drugs. Annu Rev Pharmacol Toxicol. 1984;24:175–197. doi: 10.1146/annurev.pa.24.040184.001135. [DOI] [PubMed] [Google Scholar]
  7. Högestätt E. D., Andersson K. E., Edvinsson L. Mechanical properties of rat cerebral arteries as studied by a sensitive device for recording of mechanical activity in isolated small blood vessels. Acta Physiol Scand. 1983 Jan;117(1):49–61. doi: 10.1111/j.1748-1716.1983.tb07178.x. [DOI] [PubMed] [Google Scholar]
  8. Itoh Y., Yanagisawa M., Ohkubo S., Kimura C., Kosaka T., Inoue A., Ishida N., Mitsui Y., Onda H., Fujino M. Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium-derived vasoconstrictor peptide, endothelin: identity of human and porcine endothelin. FEBS Lett. 1988 Apr 25;231(2):440–444. doi: 10.1016/0014-5793(88)80867-6. [DOI] [PubMed] [Google Scholar]
  9. Lippton H., Goff J., Hyman A. Effects of endothelin in the systemic and renal vascular beds in vivo. Eur J Pharmacol. 1988 Oct 11;155(1-2):197–199. doi: 10.1016/0014-2999(88)90424-4. [DOI] [PubMed] [Google Scholar]
  10. Lundberg J. M., Hua X. Y., Franco-Cereceda A. Effects of neuropeptide Y (NPY) on mechanical activity and neurotransmission in the heart, vas deferens and urinary bladder of the guinea-pig. Acta Physiol Scand. 1984 Aug;121(4):325–332. doi: 10.1111/j.1748-1716.1984.tb07463.x. [DOI] [PubMed] [Google Scholar]
  11. Lundberg J. M., Terenius L., Hökfelt T., Martling C. R., Tatemoto K., Mutt V., Polak J., Bloom S., Goldstein M. Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand. 1982 Dec;116(4):477–480. doi: 10.1111/j.1748-1716.1982.tb07171.x. [DOI] [PubMed] [Google Scholar]
  12. Miasiro N., Yamamoto H., Kanaide H., Nakamura M. Does endothelin mobilize calcium from intracellular store sites in rat aortic vascular smooth muscle cells in primary culture? Biochem Biophys Res Commun. 1988 Oct 14;156(1):312–317. doi: 10.1016/s0006-291x(88)80841-6. [DOI] [PubMed] [Google Scholar]
  13. Pernow J., Boutier J. F., Franco-Cereceda A., Lacroix J. S., Matran R., Lundberg J. M. Potent selective vasoconstrictor effects of endothelin in the pig kidney in vivo. Acta Physiol Scand. 1988 Dec;134(4):573–574. doi: 10.1111/j.1748-1716.1998.tb08538.x. [DOI] [PubMed] [Google Scholar]
  14. Pernow J., Lundberg J. M. Neuropeptide Y induces potent contraction of arterial vascular smooth muscle via an endothelium-independent mechanism. Acta Physiol Scand. 1988 Sep;134(1):157–158. doi: 10.1111/j.1748-1716.1988.tb08474.x. [DOI] [PubMed] [Google Scholar]
  15. Pernow J., Svenberg T., Lundberg J. M. Actions of calcium antagonists on pre- and postjunctional effects of neuropeptide Y on human peripheral blood vessels in vitro. Eur J Pharmacol. 1987 Apr 14;136(2):207–218. doi: 10.1016/0014-2999(87)90712-6. [DOI] [PubMed] [Google Scholar]
  16. Potter E. K. Prolonged non-adrenergic inhibition of cardiac vagal action following sympathetic stimulation: neuromodulation by neuropeptide Y? Neurosci Lett. 1985 Mar 15;54(2-3):117–121. doi: 10.1016/s0304-3940(85)80065-3. [DOI] [PubMed] [Google Scholar]
  17. Tatemoto K. Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5485–5489. doi: 10.1073/pnas.79.18.5485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tayo F. M., Bevan J. A. Extracellular calcium dependence of contraction and endothelium-dependent relaxation varies along the length of the aorta and its branches. J Pharmacol Exp Ther. 1987 Feb;240(2):594–601. [PubMed] [Google Scholar]
  19. Tomobe Y., Miyauchi T., Saito A., Yanagisawa M., Kimura S., Goto K., Masaki T. Effects of endothelin on the renal artery from spontaneously hypertensive and Wistar Kyoto rats. Eur J Pharmacol. 1988 Aug 2;152(3):373–374. doi: 10.1016/0014-2999(88)90736-4. [DOI] [PubMed] [Google Scholar]
  20. Wright C. E., Fozard J. R. Regional vasodilation is a prominent feature of the haemodynamic response to endothelin in anaesthetized, spontaneously hypertensive rats. Eur J Pharmacol. 1988 Oct 11;155(1-2):201–203. doi: 10.1016/0014-2999(88)90425-6. [DOI] [PubMed] [Google Scholar]
  21. Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988 Mar 31;332(6163):411–415. doi: 10.1038/332411a0. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES