Abstract
The polyoxins, nucleoside peptide antibiotics, are effective inhibitors of chitin synthesis in some fungi and yeasts. Although isolated chitin synthetases appear to be equally sensitive to inhibition by polyoxins, intact yeast cells are relatively insensitive. It has been suggested that polyoxins enter cells by a peptide carrier transport mechanism. In this paper, we report results which demonstrate that changes in the growth conditions significantly affect the degree of sensitivity of Canidida albicans to the polyoxins. For example, a minimal inhibitory concentration of 0.1 micrograms/ml was obtained in a defined medium compared with a minimal inhibitory concentration of 1,000 micrograms/ml with conventional media. Various noninhibitory di- and tripeptides, when added to the media, were found to antagonize the anti-Candida activity of polyoxins. In addition, polyoxin-resistant mutants of C. albicans were shown to exhibit cross-resistance with other dipeptide antibiotics. The data reported herein support peptide transport of polyoxins in C. albicans.
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Selected References
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- Becker J. M., Covert N. L., Shenbagamurthi P., Steinfeld A. S., Naider F. Polyoxin D inhibits growth of zoopathogenic fungi. Antimicrob Agents Chemother. 1983 Jun;23(6):926–929. doi: 10.1128/aac.23.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Braun P. C., Calderone R. A. Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms. J Bacteriol. 1978 Mar;133(3):1472–1477. doi: 10.1128/jb.133.3.1472-1477.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cabib E. Molecular aspects of yeast morphogenesis. Annu Rev Microbiol. 1975;29:191–214. doi: 10.1146/annurev.mi.29.100175.001203. [DOI] [PubMed] [Google Scholar]
- Endo A., Kakiki K., Misato T. Mechanism of action of the antifugal agent polyoxin D. J Bacteriol. 1970 Oct;104(1):189–196. doi: 10.1128/jb.104.1.189-196.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hori M., Eguchi J., Kakiki K., Misato T. Studies on the mode of action of polyoxins. VI. Effect of polyoxin B on chitin synthesis in polyoxin-sensitive and resistant strains of Alternaria kikuchiana. J Antibiot (Tokyo) 1974 Apr;27(4):260–266. doi: 10.7164/antibiotics.27.260. [DOI] [PubMed] [Google Scholar]
- Kenig M., Vandamme E., Abraham E. P. The mode of action of bacilysin and anticapsin and biochemical properties of bacilysin-resistant mutants. J Gen Microbiol. 1976 May;94(1):46–54. doi: 10.1099/00221287-94-1-46. [DOI] [PubMed] [Google Scholar]
- Kingsbury W. D., Boehm J. C., Mehta R. J., Grappel S. F. Transport of antimicrobial agents using peptide carrier systems: anticandidal activity of m-fluorophenylalanine--peptide conjugates. J Med Chem. 1983 Dec;26(12):1725–1729. doi: 10.1021/jm00366a013. [DOI] [PubMed] [Google Scholar]
- SUZUKI S., ISONO K., NAGATSU J., MIZUTANI T., KAWASHIMA Y., MIZUNO T. A NEW ANTIBIOTIC, POLYOXIN A. J Antibiot (Tokyo) 1965 May;18:131–131. [PubMed] [Google Scholar]