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. Author manuscript; available in PMC: 2008 Jan 12.
Published in final edited form as: Cell. 2007 Jan 12;128(1):141–156. doi: 10.1016/j.cell.2006.11.040

Figure 5. Mono-ubiquitination regulates PTEN nuclear import and shuttling.

Figure 5

(A) FRAP assay measuring effect of ubiquitin overexpression on PTEN import (left panel) and quantification of nuclear and cytoplasmic PTEN accumulation rates (right panel). Error bars are S. D.

(B) Nuclear/ cytoplasmic fractionation of PTEN after ubiquitin overexpression. Lamin B1 and α-Tubulin serve as nuclear and cytoplasmic markers, respectively. Numbers denote nuclear to cytoplasmic PTEN ratios (relative to Lamin and Tubulin, see Supplemental Data). A non PTEN-specific band is indicated (n).

(C) Immunofluorescence imaging of PTEN and K289E with ubiquitin co-expression. Scale bar, 10 μm.

(D) Ubiquitination rescues K289E nuclear import defect as measured by FRAP (left panel; quantifications: right panel). Note that mono-ubiquitination is most efficient in rescuing. Error bars show S. D.