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. 1984 May;25(5):646–649. doi: 10.1128/aac.25.5.646

Size and stability of a naturally occurring virus inhibitor.

D H Coppenhaver, J L Baron, M L McKerlie, J Sabados, S Baron
PMCID: PMC185606  PMID: 6732231

Abstract

We recently described a virus inhibitor (contact-blocking virus inhibitor) which was produced spontaneously by untransformed human and murine cells in tissue culture (S. Baron and L. McKerlie , Infect. Immun . 32:449-453, 1981). This contact-blocking virus inhibitor was characterized by broad antiviral activity, high potency, and reversible inhibition of viral attachment. Unlike interferon, the antiviral activity of the contact-blocking virus inhibitor is not species specific. An inhibitor with similar properties can also be demonstrated in many body fluids and surface secretions. We report here studies on the stability of the antiviral species which indicate that it is resistant to denaturation by heat (100 degrees C), acid (pH 2), and alkali (pH 12). The antiviral activity against all viruses tested resides in a low-molecular-weight molecule. The range of characteristics so far determined for the contact-blocking virus inhibitor distinguishes it from other virus inhibitors reported in the literature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baron S., McKerlie L. Broadly active inhibitor of viruses spontaneously produced by many cell types in culture. Infect Immun. 1981 May;32(2):449–453. doi: 10.1128/iai.32.2.449-453.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Falkler W. A., Jr, Diwan A. R., Halstead S. B. A lipid inhibitor of dengue virus in human colostrum and milk; with a note on the absence of anti-dengue secretory antibody. Arch Virol. 1975;47(1):3–10. doi: 10.1007/BF01315587. [DOI] [PubMed] [Google Scholar]
  3. Gupta P., Ferrer J. F. Expression of bovine leukemia virus genome is blocked by a nonimmunoglobulin protein in plasma from infected cattle. Science. 1982 Jan 22;215(4531):405–407. doi: 10.1126/science.6276975. [DOI] [PubMed] [Google Scholar]
  4. Hughes T. K., Blalock J. E., McKerlie M. L., Baron S. Cell-produced viral inhibitor: possible mechanism of action and chemical composition. Infect Immun. 1981 May;32(2):454–457. doi: 10.1128/iai.32.2.454-457.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Krizanová O., Rathová V. Serum inhibitors of myxoviruses. Curr Top Microbiol Immunol. 1969;47:125–151. doi: 10.1007/978-3-642-46160-6_6. [DOI] [PubMed] [Google Scholar]
  6. LIEBHABER H., TAKEMOTO K. K. Alteration plaque morphology of EMC virus with polycations. Virology. 1961 Aug;14:502–504. doi: 10.1016/0042-6822(61)90349-x. [DOI] [PubMed] [Google Scholar]
  7. LIEBHABER H., TAKEMOTO K. K. THE BASIS FOR THE SIZE DIFFERENCES IN PLAQUES PRODUCED BY VARIANTS OF ENCEPHALOMYOCARDITIS (EMC) VIRUS. Virology. 1963 Aug;20:559–566. doi: 10.1016/0042-6822(63)90280-0. [DOI] [PubMed] [Google Scholar]
  8. Leong J. C., Kane J. P., Oleszko O., Levy J. A. Antigen-specific nonimmunoglobulin factor that neutralizes xenotropic virus is associated with mouse serum lipoproteins. Proc Natl Acad Sci U S A. 1977 Jan;74(1):276–280. doi: 10.1073/pnas.74.1.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levy J. A., Ihle J. N., Oleszko O., Barnes R. D. Virus-specific neutralization by a soluble non-immunoglobulin factor found naturally in normal mouse sera. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5071–5075. doi: 10.1073/pnas.72.12.5071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Matthews T. H., Nair C. D., Lawrence M. K., Tyrrell D. A. Antiviral activity in milk of possible clinical importance. Lancet. 1976 Dec 25;2(8000):1387–1389. doi: 10.1016/s0140-6736(76)91922-x. [DOI] [PubMed] [Google Scholar]
  11. TAKEMOTO K. K., FABISCH P. INFLUENCE OF ACID POLYSACCHARIDES ON PLAQUE FORMATION BY INFLUENZA A2 AND B VIRUSES. Proc Soc Exp Biol Med. 1963 Dec;114:811–814. doi: 10.3181/00379727-114-28806. [DOI] [PubMed] [Google Scholar]
  12. TAKEMOTO K. K., FABISCH P. INHIBITION OF HERPES VIRUS BY NATURAL AND SYNTHETIC ACID POLYSACCHARIDES. Proc Soc Exp Biol Med. 1964 May;116:140–144. doi: 10.3181/00379727-116-29183. [DOI] [PubMed] [Google Scholar]
  13. TAMM I., HORSFALL F. L., Jr A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J Exp Med. 1952 Jan;95(1):71–97. doi: 10.1084/jem.95.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Welsh J. K., Arsenakis M., Coelen R. J., May J. T. Effect of antiviral lipids, heat, and freezing on the activity of viruses in human milk. J Infect Dis. 1979 Sep;140(3):322–328. doi: 10.1093/infdis/140.3.322. [DOI] [PubMed] [Google Scholar]

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