Lactobacillus farciminis treatment suppresses stress induced visceral hypersensitivity: a possible action through interaction with epithelial cell cytoskeleton contraction

Abstract

Background

Stress induced increase in colonic paracellular permeability results from epithelial cell cytoskeleton contraction and is responsible for stress induced hypersensitivity to colorectal distension (CRD). The probiotic Lactobacillus farciminis releases spontaneously nitric oxide (NO) in the colonic lumen in vivo and exerts anti‐inflammatory effects. This study aimed: (i) to evaluate the effects of L farciminis on stress induced hypersensitivity to CRD and increase in colonic paracellular permeability; and (ii) to ascertain whether these effects are NO mediated and related to changes in colonocyte myosin light chain phosphorylation (p‐MLC).

Methods

Female Wistar rats received either 10¹¹ CFU/day of L farciminis or saline orally over 15 days before partial restraint stress (PRS) or sham‐PRS application. Visceral sensitivity to CRD and colonic paracellular permeability was assessed after PRS or sham‐PRS. Haemoglobin was used as an NO scavenger. Western blotting for MLC kinase, MLC, and p‐MLC were performed in colonic mucosa from L farciminis treated and control rats after PRS or sham‐PRS.

Results

PRS significantly increased the number of spike bursts for CRD pressures of 30–60 mm Hg as well as colonic paracellular permeability. L farciminis treatment prevented both effects, while haemoglobin reversed the protective effects of L farciminis. p‐MLC expression increased significantly from 15 to 45 minutes after PRS, and L farciminis treatment prevented this increase.

Conclusion

L farciminis treatment prevents stress induced hypersensitivity, increase in colonic paracellular permeability, and colonocyte MLC phosphorylation. This antinociceptive effect occurs via inhibition of contraction of colonic epithelial cell cytoskeleton and the subsequent tight junction opening, and may also involve direct or indirect effects of NO produced by this probiotic.

Probiotics have been defined as living organisms in food and dietary supplements which improve the health of the host beyond inherent basic nutrition.¹ Preventive and curative efficacy of probiotic treatments has been described in several gastrointestinal pathologies and in the past decade encouraging results have been obtained in inflammatory bowel disease using different strains.²,³ Similarly, treatment with several lactobacilli strains has been found to reduce the severity of experimental colitis in animals.⁴,⁵ Based on a recent approach using bacteria as a carrier of anti‐inflammatory compounds delivered into the colonic lumen,⁶ we have previously shown that Lactobacillus farciminis given orally for 15 days can release spontaneously nitric oxide (NO) into the colonic lumen and reduce the severity of trinitrobenzene sulphonic acid (TNBS) induced colitis in rats.⁷ Among the targets which have benefited from probiotics, studies have reported enhancement of intestinal barrier function⁸,⁹,¹⁰ by strengthening tight junctions between enterocytes¹¹,¹²,¹³ and thereby preventing an increase in paracellular permeability and subsequent bacterial translocation. L farciminis shares these properties as it prevents bacterial translocation and the increase in colonic paracellular permeability in TNBS induced colitis in rats.⁵

On the other hand, irritable bowel syndrome is a gastrointestinal disease with unknown aetiology, frequently associated with psychological distress, and characterised by abdominal pain.¹⁴,¹⁵ An increase in intestinal permeability has recently been described in post‐dysenteric¹⁶ and other irritable bowel syndrome patients.¹⁷ Manifestations such as increased gut permeability and visceral hypersensitivity are similar to those initiated by various stressful stimuli in rats and mice.¹⁸,¹⁹,²⁰,²¹ In rats, partial restraint stress induces colonic hypersensitivity to distension triggered by an increase in gut paracellular permeability.²¹ Moreover, this stress induced increase in gut permeability results from epithelial cell cytoskeleton contraction.¹⁹,²¹ In contrast, despite controversies in the literature concerning the role of NO in pain transmission and mucosal barrier protection, an antinociceptive action of NO in visceral or peritoneal pain²²,²³,²⁴ as well as improvement in mucosal barrier function by NO donors²⁵ have already been described.

Based on this background, the aim of our study was to evaluate: (i) whether L farciminis treatment prevents stress induced visceral hypersensitivity to colorectal distension (CRD) and increases colonic paracellular permeability; (ii) the involvement of NO in these effects; and (iii) the effect of L farciminis treatment on stress induced changes in colonocyte phosphorylated myosin light chain (p‐MLC) expression, reflecting contraction of the cytoskeleton.

Materials and methods

Animals

Female Wistar rats (Janvier SA, Le Genest St Isle, France) weighing 200–225 g and housed individually in a temperature controlled room (21±1°C) were used. They were allowed free access to water and fed standard pellets (UAR pellets; Epinay, France). The local committee for animal use and care approved all experimental protocols described in this study.

Bacteria preparation

L farciminis (CIP 103136; Institut Pasteur Collection, Paris, France) was grown at 37°C in MRS broth (VWR International, Fontenay‐sous‐Bois, France). After 17 hours of incubation, cultures were harvested by centrifugation at 4500 g for 10 minutes. Strain extract was resuspended in 0.9% NaCl and conserved at −20°C. Bacterial suspension was prepared daily in order to administer orally 10¹¹ CFU/day/rat.

Animal preparation

Under general anaesthesia induced by intraperitoneal administration of 0.6 mg/kg acepromazine (Calvimet; Vetoquinol, Lure, France) and 120 mg/kg ketamine (Imalgene 1000; Rhône‐Mérieux, Lyon, France), animals were equipped with a polyethylene catheter (OD 0.7 mm, ID 0.3 mm, length 60 cm) inserted into the proximal colon at 1 cm from the caecocolonic junction. Rats were also equipped with three groups of three NiCr wire electrodes (60 cm in length, 80 µm in diameter) implanted into the abdominal external oblique muscle, 2 cm superior to the inguinal ligament.²⁶ Catheter and electrodes were exteriorised on the back of the neck and protected by a glass tube attached to the skin. Myoelectrical activity was recorded on an electromyograph (Mini VIII; Alvar, Paris, France) using a short time constant (0.03 seconds) to remove low frequency signals (<3 Hz) and with a paper speed of 3.6 cm/min.

Partial restraint stress

All stress sessions were performed at the same time of day (between 10am and 12 noon) to minimise any influence of circadian rhythms. Stress effects were studied using the wrap partial restraint stress (PRS) model which is a mild non‐ulcerogenic stressor.²⁷ Thus animals were lightly anaesthetised with ethyl‐ether and their fore shoulders, upper forelimbs, and thoracic trunk were wrapped in a confining harness of paper tape to restrict, but not to prevent, body movements. Then, rats were placed in their home cage for two hours. Rats recovered from ethyl‐ether within 2–3 minutes and immediately moved around in their cage, although the restricted mobility of their forelimb prevented grooming behaviour. Sham‐PRS rats, considered as controls, were anaesthetised as above but were not wrapped and were allowed to move freely in their cages.

Colorectal distension

Rats were accustomed to being in polypropylene tunnels (diameter 7 cm; length 20 cm) for several days before CRD in order to minimise recording artefacts. A 4 cm long latex balloon fixed on a rigid catheter was used. CRD was performed by insertion of the balloon into the rectum 1 cm from the anus. Isobaric distensions of the colon were performed by connecting the balloon to a computerised barostat. Colonic pressure and balloon volume were continuously monitored on a potentiometric recorder (L6514; Linseis, Selb, Germany). Isobaric distensions were performed from 0 to 60 mm Hg, each distension step lasting five minutes. The first distension was performed at a pressure of 15 mm Hg and an increment of 15 mm Hg was added at each following step until a maximal pressure of 60 mmHg.

Colonic paracellular permeability

Colonic paracellular permeability was evaluated using ⁵¹Cr‐ethylenediaminetetraacetic acid (EDTA; Perkin Elmer Life Sciences, Paris, France) as a marker of paracellular permeation of tight junctions.²⁸⁵¹Cr‐EDTA (0.7 µCi), diluted in 250 µl of saline, was injected through the intracolonic catheter 20 minutes after PRS or sham‐PRS. Then, animals were placed in metabolic cages, and faeces and urine were collected separately over 24 hours. Radioactivity in urine was measured on a gamma counter (Cobra II; Packard, Meriden, Connecticut, USA). Permeability to ⁵¹Cr‐EDTA was expressed as percentage of administered radioactivity recovered in urine.

Western blot analysis for myosin light chain kinase (MLCK), myosin light chain (MLC), and p‐MLC

After sacrifice, a 2 cm segment of proximal colon was removed, washed with saline, and the colonic mucosa was scraped off. Proteins were extracted in RIPA buffer and protein concentrations assessed using the BC Assay kit (Interchim, Montluçon, France). Equal amounts of each extract were subjected to 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis and then electrotransferred onto PVDF membrane. Membranes were blocked overnight at 4°C with Tris buffered saline /bovine serum albumin 3%, before incubation with primary antibodies. Immunoblotting was performed using specific anti‐MLC, anti‐MLCK (Sigma, Saint Quentin Fallavier, France), and anti‐phospho‐MLC (Clinisciences, Montrouge, France) antibodies at the following dilutions: 1:500, 1:500, and 1:10 000, respectively. After washing, horseradish peroxidase conjugated secondary antibody was used (Upstate, Charlottesville, Virginia, USA). Bands were identified by ECL detection reagents (Amersham Biosciences Europe GmbH, Orsay, France). Band intensities were quantified by densitometry and expressed as mean area density using Quantify one 4.1.1 software.

Experimental protocol

In the first series of experiments, 10 groups of eight rats were used. Over 15 days, animals received orally either saline (groups 1, 2, 4, 5, 7, and 9) or 10¹¹CFU/day L farciminis (groups 3, 6, 8, and 10). Animals were equipped with NiCr electrodes implanted in the abdominal striated muscles (groups 1, 2, 3, 7, and 8) or an intracolonic catheter (groups 4, 5, 6, 9, and 10).

Rats were subjected to PRS (groups 2, 3, 5, 6, 7, 8, 9, and 10) or sham‐PRS (groups 1 and 4). Stepwise CRD were performed in groups 1, 2, 3, 7, and 8, 20 minutes after PRS or sham‐PRS. Groups 4, 5, 6, 9, and 10 received ⁵¹Cr‐EDTA intracolonically at the end of PRS or sham‐PRS session, and colonic paracellular permeability was assessed by 24 hours post‐PRS urine collection. Groups 7–10 were continuously infused with haemoglobin, an NO scavenger (0.25 ml/h; 200 mg/kg/day) through the intracolonic catheter over 48 hours before PRS until the end of the CRD session (groups 7 and 8) or until the end of the urine collection period (groups 9 and 10).

In a second series of experiments, 12 groups of four rats were used for the kinetics of MLCK, MLC, and p‐MLC protein expression of stress exposure by western immunoblotting. Groups 1–6 and groups 7–12 were subjected to sham‐PRS and PRS, respectively. After 15, 30, 45, 60, 75, and 90 minutes of sham‐PRS or PRS application, the corresponding group was sacrificed and colonic segments were removed for western blot assays.

In the last series of experiments and according to the results obtained concerning the effect after 45 minutes of PRS application on MLC phosphorylation, four groups of four rats were used in order to evaluate the influence of L farciminis treatment on these stress induced changes. Saline (groups 1 and 2) or 10¹¹ CFU/ml L farciminis were given orally over 15 days before sham‐PRS or PRS, respectively. All groups were sacrificed at 45 minutes after sham‐PRS or PRS application and colonic segments were removed for p‐MLC protein level determination by western blotting.

Statistical analysis

All values are expressed as mean (SEM). Statistical analysis of the number of spike bursts for each five minute period during CRD and colonic paracellular permeability were performed using one way analysis of variance. Differences were considered significant when post hoc test p<0.05.

Results

L farciminis and stress induced visceral hypersensitivity

PRS significantly (p<0.05) increased the number of spike bursts for CRD pressures of 30–60 mm Hg compared with sham‐PRS (50 (5) v 29 (3) spike bursts/5 min at 45 mm Hg) (fig 1A). L farciminis treatment prevented the increase in the number of spike bursts induced by PRS, whatever the pressure of CRD (fig 1A). The beneficial effect of L farciminis treatment on stress induced visceral hyperalgesia was abolished when animals received haemoglobin infusion (54 (3) v 28 (2) spike bursts/5 min at 45 mm Hg) (fig 1B). Haemoglobin infusion had no effect per se on PRS induced increase in the number of spike bursts (data not shown).

Figure 1 (A) Effect of Lactobacillus farciminis (10¹¹ CFU/day) treatment on stress induced hypersensitivity in rats. *p<0.05, significantly different from sham‐PRS; †p<0.05, significantly different from partial restraint stress (PRS). (B) Reversion by haemoglobin (200 mg/kg/day) of the effect of L farciminis treatment (10¹¹ CFU/day) on hyperalgesia induced by PRS. *p<0.05 significantly different from PRS+L farciminis.

L farciminis and stress induced increase in colonic paracellular permeability

PRS significantly (p<0.05) increased colonic paracellular permeability (2.07 (0.24) v 1.20 (0.20)%) compared with sham‐PRS (fig 2). L farciminis treatment prevented the increase in colonic paracellular permeability induced by PRS (0.95 (0.09) v 2.07 (0.24)%) (fig 2). Haemoglobin infusion reversed the effect of L farciminis on the increase in colonic paracellular permeability induced by PRS (1.78 (0.18) v 2.07 (0.24) %) (fig 2). Haemoglobin infusion did not affect the increase in colonic paracellular permeability induced by PRS (data not shown).

Figure 2 Effect of Lactobacillus farciminis (10¹¹ CFU/ml/day) treatment on the increase in colonic paracellular permeability induced by partial restraint stress (PRS) and its reversion by haemoglobin (Hb 200 mg/kg/day). *p<0.05, significantly different from sham‐PRS; †p<0.05 significantly different from PRS; ‡p<0.05 significantly different from PRS+L farciminis. EDTA, ethylenediaminetetraacetic acid.

Time dependent changes in MLC and p‐MLC protein levels after stress exposure

MLCK protein levels were not affected by exposure to stress (table 1). Conversely, significantly (p<0.05) higher levels of p‐MLC were observed from 15 to 45 minutes after the beginning of stress application, with a maximal effect at 45 minutes (fig 3). Then, p‐MLC levels decreased and returned to basal values at 75 minutes after the beginning of stress application (fig 3). MLC protein levels were similar to those observed in sham stressed rats during the early stages of stress application but they increased significantly (p<0.05) after 60 minutes from the beginning of the stress session (table 1).

Table 1 Kinetics of myosin light chain kinase (MLCK) and myosin light chain (MLC) protein level determination after sham‐partial restraint stress (PRS) and PRS exposure*.

		Times of sham‐PRS or PRS exposure (minutes)
		15	30	45	60	75	90
MLCK	Sham‐PRS	0.49 (0.01)	0.47 (0.02)	0.45 (0.03)	0.48 (0.02)	0.50 (0.01)	0.47 (0.02)
	PRS	0.48 (0.01)	0.52 (0.02)	0.54 (0.03)	0.54 (0.03)	0.48 (0.01)	0.49 (0.01)
MLC	Sham‐PRS	0.49 (0.01)	0.47 (0.02)	0.45 (0.03)	0.48 (0.02)	0.50 (0.01)	0.48 (0.02)
	PRS	0.44 (0.05)	0.53 (0.01)	0.56 (0.03)	0.83 (0.03)†	0.82 (0.03)†	0.68 (0.04)†

Data are represented as mean (SEM) area density.

*By western blot band density.

†p<0.05 versus sham‐PRS.

Figure 3 Western blot for evaluation of phosphorylated myosin light chain (p‐MLC) protein levels on colonic mucosa after partial restraint stress (PRS) application for different time periods. (A) Western immunoblotting for p‐MLC. (B) Relative mean (SEM) area density. Significant increases in p‐MLC protein levels were observed from 15 to 45 minutes after the beginning of stress application. **p<0.01 significantly different from sham‐PRS.

L farciminis treatment effect on changes in p‐MLC protein levels induced by stress

In sham treated animals, L farciminis did not change p‐MLC levels. Conversely, after 45 minutes of stress exposure, L farciminis treatment significantly (p<0.05) decreased p‐MLC protein levels (fig 4).

Figure 4 Western blot for phosphorylated myosin light chain (p‐MLC) protein levels on colonic mucosa after Lactobacillus farciminis treatment. (A) Western immunoblotting for p‐MLC. (B) Relative mean (SEM) area density. L farciminis treatment reduced phosphorylation of MLC induced by 45 minutes of stress exposure. Significantly different (p<0.05) from *sham‐PRS and †PRS, respectively.

Discussion

The present study shows that oral L farciminis treatment prevents stress induced visceral hypersensitivity to colorectal distension and the increase in colonic paracellular permeability. Delivery of exogenous NO by this strain into the colonic lumen is involved in these protective effects as they are both reversed by intracolonic infusion of haemoglobin, an NO scavenger. Moreover, L farciminis treatment suppresses stress induced colonocyte MLC phosphorylation, reflecting their cytoskeleton contraction.

In animals, acute restraint stress enhances abdominal responses to rectal distension¹⁸,²¹,²⁹ and we have recently established that this hypersensitivity to distension results from increased gut permeability.²¹ In a previous study, we showed that L farciminis, given orally for 15 days, releases spontaneously NO into the colonic lumen.⁷ We can hypothesise that NO released luminally by L farciminis may act on colonic permeability. A protective effect of NO on mucosal barrier permeability has been described. Indeed, alterations in mucosal barrier permeability after ischaemic injury are also reduced in rats pretreated with molsidomine, an NO donor.²⁵ Furthermore, it has been shown that administration of NO synthase inhibitors elicits large increases in the mucosal permeability of the feline intestine, independent of alterations in intestinal blood flow or adhesion of leucocytes to vascular endothelium.³⁰ This effect of NO synthase inhibitors is reversed by simultaneous administration of NO donor or L‐arginine, underlying a modulatory role of NO produced in the gut mucosa on paracellular permeability.³⁰,³¹

Increased colonic paracellular permeability resulting from stress depends on epithelial cell cytoskeleton contraction through MLCK activation, inducing MLC phosphorylation, as a specific MLCK inhibitor (ML‐7) prevents the stress induced increase in permeability in mice¹⁹ and rats.²¹ In this study, we showed that acute stress induced MLC phosphorylation at early stages of stress application, and L farciminis treatment suppressed this effect. Interestingly, in a recent study, NO was found to be involved in the modulation of intestinal permeability by preventing MLC phosphorylation in vitro.³² In contrast, the role of NO in pain transmission is controversial. NO has been proposed as being involved in inflammatory hyperalgesia³³ but it is also considered a mediator of opiate analgesia.³⁴ Only a few studies have investigated the role of NO on visceral or peritoneal pain models and they indicate an antinociceptive action of NO.²²,²³,²⁴ Taken together, these data suggest that the antinociceptive effect of L farciminis on stress induced hyperalgesia is probably due to a primary regulatory action of NO on colonic permeability rather than a direct action of NO on nociceptive fibres.

The hypothesis of NO involvement in the protective effects of L farciminis on both colonic paracellular permeability and visceral sensitivity is based on reversal of these effects by intracolonic infusion of haemoglobin. NO is known to form dinitrosyl complexes with iron and to bind easily and strongly with proteins that contain the haem group, such as haemoglobin.³⁵ Consequently, haemoglobin has been used as an appropriate NO scavenger in several experimental studies.³⁶,³⁷ However, we cannot exclude the fact that haemoglobin infusion in the rat colon may impact on the colonic microecology in terms of specific bacterial metabolic activity and/or population composition changes, suggesting that observations obtained in animals undergoing haemoglobin infusion may result from a direct action of haemoglobin on colonic microbiota. Haemoglobin or other haemoproteins reaching the colonic lumen are metabolised by bacteria to a range of haem derived porphyrins lacking iron.³⁸ In a previous work we have shown that L farciminis significantly increased colonic NO concentrations and haemoglobin infusion reduced these concentrations to basal levels, similar to saline treated rats, suggesting that there are no significant changes concerning the ability of the microflora to degrade haemoglobin as this haemoprotein remains able to scavenge NO. However, further investigations are needed to confirm the involvement of NO by using alternative NO scavengers, and also to exclude the possibility that the effect of haemoglobin is due to another mechanism, such as alteration of the microflora.

The protective effect of L farciminis treatment on mucosal barrier integrity is in agreement with previous data showing that in vitro live probiotic strains interact with intestinal epithelial cells to protect them from the deleterious effects of enteroinvasive Escherichia coli by blocking the fall in transepithelial resistance and acting on tight junction proteins.¹² Similarly, supernatant of Lactobacillus thermophilus culture prevents aspirin induced inhibition of ZO‐1 expression.¹³ We therefore speculate that blockade of tight junction opening by L farciminis during stress may in turn prevent excessive uptake of luminal microbial antigens and bacterial products able to activate the submucosal immune system, which may sensitise terminals or sensory nerves through release of mediators. According to the literature, the most attractive potential use of probiotics seems to be colonisation of the gut and subsequent improvement of the intestinal microflora balance and fight against pathogens. We have previously shown that L farciminis given orally for two weeks can adhere to the mucosa of the proximal colon and modulate the immunity of the inflamed colon in rats.⁵ Thus we cannot exclude the fact that the ability to colonise and interact with the microflora could be involved in the beneficial effects of L farciminis treatment observed in this study. Furthermore, a recent study by Verdu et al showed that perturbations in the gut flora and inflammatory cell activity enhanced visceral sensitivity and this effect was prevented by Lactobacillus paracasei administration.³⁹ Also, a preliminary report from our group showed that disturbances of the commensal flora resulting from oral antibiotic administration in mice decreased the effects of acute stress induced changes on colonic permeability, underlying the role of the flora in the responsiveness of epithelial cells.⁴⁰

In conclusion, in this study we showed that probiotic treatment prevented the acute stress induced hypersensitivity to distension and the increase in colonic paracellular permeability. It is possible that NO released by L farciminis may affect directly or indirectly colonic paracellular permeability and subsequent colonic hypersensitivity but further investigations are needed to confirm this action. Finally, L farciminis exert a primary action on the colonic epithelial barrier and the effect at this level results from inhibition of MLC phosphorylation and cytoskeleton contraction. All of these data suggest that L farciminis may be of interest in the treatment of visceral hyperalgesia, particularly irritable bowel syndrome.

Abbreviations

CRD - colorectal distension

EDTA - ethylenediaminetetraacetic acid

MLC - myosin light chain

MLCK - myosin light chain kinase

p‐MLC - phosphorylated myosin light chain

NO - nitric oxide

PRS - partial restraint stress

TNBS - trinitrobenzene sulphonic acid