Abstract
A newly developed homogeneous enzyme immunoassay for the determination of netilmicin in serum was evaluated and compared with a radioenzymatic assay. A total of 102 serum samples from patients treated with netilmicin were measured by both methods. This comparison showed an excellent correlation (r = 0.993). The enzyme immunoassay has proved to be precise, accurate, and specific. Because of its rapidity and the ease of performance, this method is a useful alternative to current assays for monitoring serum netilmicin concentrations.
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Selected References
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- Broughall J. M., Reeves D. S. The acetyltransferase enzyme method for the assay of serum gentamicon concentrations and a comparison with other methods. J Clin Pathol. 1975 Feb;28(2):140–145. doi: 10.1136/jcp.28.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brummett R. E., Fox K. E., Brown R. T., Himes D. L. Comparative ototoxic liability of netilmicin and gentamicin. Arch Otolaryngol. 1978 Oct;104(10):579–584. doi: 10.1001/archotol.1978.00790100033007. [DOI] [PubMed] [Google Scholar]
- Follath F., Wenk M., Vozeh S. Plasma concentration monitoring of aminoglycosides. J Antimicrob Chemother. 1981 Jul;8 (Suppl A):37–43. doi: 10.1093/jac/8.suppl_a.37. [DOI] [PubMed] [Google Scholar]
- Francke E. L., Srinivasan S., Labthavikul P., Neu H. C. Rapid, reproducible enzyme immunoassay for tobramycin. J Clin Microbiol. 1981 Jan;13(1):93–96. doi: 10.1128/jcm.13.1.93-96.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luft F. C., Yum M. N., Kleit S. A. Comparative nephrotoxicities of netilmicin and gentamicin in rats. Antimicrob Agents Chemother. 1976 Nov;10(5):845–849. doi: 10.1128/aac.10.5.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maitra S. K., Yoshikawa T. T., Guze L. B., Schotz M. C. Determination of aminoglycoside antibiotics in biological fluids: a review. Clin Chem. 1979 Aug;25(8):1361–1367. [PubMed] [Google Scholar]
- O'Leary T. D., Ratcliff R. M., Geary T. D. Evaluation of an enzyme immunoassay for serum gentamicin. Antimicrob Agents Chemother. 1980 May;17(5):776–778. doi: 10.1128/aac.17.5.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart D., Bodey G. P., LeBlanc B. In vitro studies on netilmicin, a new aminoglycoside antibiotic. Antimicrob Agents Chemother. 1977 Jun;11(6):1017–1020. doi: 10.1128/aac.11.6.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Voegeli C. J., Vurckart G. J. Improving the sensitivity of gentamicin enzyme immunoassay. Clin Chem. 1982 Jan;28(1):248–248. [PubMed] [Google Scholar]
- Wenk M. Concepts for aminoglycoside serum level monitoring. J Antimicrob Chemother. 1982 Mar;9(3):168–169. doi: 10.1093/jac/9.3.168. [DOI] [PubMed] [Google Scholar]
- Wenk M., Spring P., Vozeh S., Follath F. Multicompartment pharmacokinetics of netilmicin. Eur J Clin Pharmacol. 1979 Nov;16(5):331–334. doi: 10.1007/BF00605631. [DOI] [PubMed] [Google Scholar]