Abstract
A radioimmunoassay system has been developed for the measurement of two major metabolites of 9,3"-diacetylmidecamycin, Mb-6 and Mb-12. A radioimmunoassay for Mb-6 was performed by using anti-Mb-6 serum and a [125I]tyramined Mb-6 derivative as a radiolabeled antigen. The labeled antigen was prepared by the chloramine T method. The antiserum was obtained from a rabbit immunized with Mb-6 conjugated to bovine serum albumin. The obtained antiserum was cross-reactive with two other metabolites of 9,3"-diacetylmidecamycin, Mb-2 and Mb-12, in addition to Mb-6. This Mb-6 radioimmunoassay system could detect Mb-6 concentrations as low as 100 pg/ml of serum. The coefficients of variation were 4.5% (intra-assay) and 5.1% (inter-assay). A radioimmunoassay for Mb-12, using anti-midecamycin serum and a [125I]tyramined-Mb-12 derivative, has also been developed. The antiserum was cross-reactive only with Mb-12 among the 9,3"-diacetylmidecamycin metabolites. This Mb-12 radioimmunoassay system could detect Mb-12 concentrations as low as 2 ng/ml. The intra- and inter-assay variances were 5.9 and 5.8%, respectively.
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- HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
- Omoto S., Iwamatsu K., Inouye S., Niida T. Modifications of a macrolide antibiotic midecamycin (SF-837). I. Synthesis and structure of 9,3''-diacetylmidecamycin. J Antibiot (Tokyo) 1976 May;29(5):536–548. doi: 10.7164/antibiotics.29.536. [DOI] [PubMed] [Google Scholar]
- Shomura T., Someya S., Murata S., Umemura K., Nishi M. Metabolism of 9,3"-diacetylmidecamycin. II. The structures of several metabolites of 9,3"-diacetylmidecamycin. Chem Pharm Bull (Tokyo) 1981 Sep;29(9):2413–2419. doi: 10.1248/cpb.29.2413. [DOI] [PubMed] [Google Scholar]
- Shomura T., Someya S., Umemura K., Nishio M., Murata S. [Metabolism of 9,3"-diacetylmidecamycin. I. The metabolic fate of 9,3"-diacetylmidecamycin]. Yakugaku Zasshi. 1982 Aug;102(8):781–795. doi: 10.1248/yakushi1947.102.8_781. [DOI] [PubMed] [Google Scholar]
- Shomura T., Umemura K. Studies on absorption, distribution, metabolism and excretion of a new macrolide antibiotic SF-837. I. Absorption, metabolism and excretion of SF-837. Chem Pharm Bull (Tokyo) 1973 Aug;21(8):1824–1831. doi: 10.1248/cpb.21.1824. [DOI] [PubMed] [Google Scholar]