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. 1983 Nov;24(5):689–695. doi: 10.1128/aac.24.5.689

Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli.

R N Rao, N E Allen, J N Hobbs Jr, W E Alborn Jr, H A Kirst, J W Paschal
PMCID: PMC185926  PMID: 6318654

Abstract

A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The specific modification of hygromycin B is a phosphorylation of the hydroxyl on the 4 position of the cyclitol ring (hyosamine). The presence of the phosphotransferase in E. coli correlates with reduced accumulation of [14C]hygromycin B.

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Selected References

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