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. 1994 Jan;62(1):172–177. doi: 10.1128/iai.62.1.172-177.1994

Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops.

J Minami 1, S Katayama 1, O Matsushita 1, H Sakamoto 1, A Okabe 1
PMCID: PMC186083  PMID: 8262625

Abstract

We examined the enterotoxicity of a Klebsiella oxytoca cytotoxin which is produced by K. oxytoca OK-1, a strain from a patient with antibiotic-associated hemorrhagic colitis. Injection of the cytotoxin into ligated ileal and colonic loops in rabbits caused the accumulation of fluid in the loops. The fluid was bloody in the ileal loops but not in the colonic ones. Histological examination revealed intense mucosal hemorrhage with erosion in the ileum, whereas no microscopic change was noted in the colon. The fluid accumulation was shown to be a dose-dependent response in both ileal and colonic loops. The amounts of the cytotoxin required for maximal fluid accumulation in ileal and colonic loops were 60 and 10 micrograms, respectively. Fluid accumulation was first noticeable in ileal loops 12 h and in colonic ones 5 h after the injection of these doses of the cytotoxin and then proceeded with time. When K. oxytoca OK-1, a cytotoxin-producing strain, was inoculated into the loops at doses of 1 x 10(8) and 5 x 10(9) CFU, similar fluid accumulation was observed. However, inoculation of K. oxytoca ATCC 13182, a non-cytotoxin-producing strain, at the same doses did not cause any change. These results suggest that the cytotoxin-producing strain of K. oxytoca is the causative organism of antibiotic-associated hemorrhagic colitis and that the toxin is the factor responsible for pathogenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banno Y., Kobayashi T., Kono H., Watanabe K., Ueno K., Nozawa Y. Biochemical characterization and biologic actions of two toxins (D-1 and D-2) from Clostridium difficile. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S11–S20. doi: 10.1093/clinids/6.supplement_1.s11. [DOI] [PubMed] [Google Scholar]
  2. Bartlett J. G., Chang T. W., Gurwith M., Gorbach S. L., Onderdonk A. B. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N Engl J Med. 1978 Mar 9;298(10):531–534. doi: 10.1056/NEJM197803092981003. [DOI] [PubMed] [Google Scholar]
  3. Chida T., Nakaya R., Tsuji M., Shimizu N., Masuda G., Seo T., Sagara H., Matsubara Y. [Intestinal microflora of patients with antibiotic-associated hemorrhagic colitis associated with Klebsiella oxytoca and Clostridium difficile enterotoxin]. Kansenshogaku Zasshi. 1986 Jun;60(6):608–615. doi: 10.11150/kansenshogakuzasshi1970.60.608. [DOI] [PubMed] [Google Scholar]
  4. DE S. N. Enterotoxicity of bacteria-free culture-filtrate of Vibrio cholerae. Nature. 1959 May 30;183(4674):1533–1534. doi: 10.1038/1831533a0. [DOI] [PubMed] [Google Scholar]
  5. Fiorentini C., Thelestam M. Clostridium difficile toxin A and its effects on cells. Toxicon. 1991;29(6):543–567. doi: 10.1016/0041-0101(91)90050-2. [DOI] [PubMed] [Google Scholar]
  6. Gorbach S. L., Bartlett J. G. Pseudomembranous enterocolitis: a review of its diverse forms. J Infect Dis. 1977 Mar;135 (Suppl):S89–S94. doi: 10.1093/infdis/135.supplement.s89. [DOI] [PubMed] [Google Scholar]
  7. Gould P. C., Khawaja F. I., Rosenthal W. S. Antibiotic-associated hemorrhagic colitis. Am J Gastroenterol. 1982 Jul;77(7):491–493. [PubMed] [Google Scholar]
  8. Guerrant R. L., Dickens M. D., Wenzel R. P., Kapikian A. Z. Toxigenic bacterial diarrhea: nursery outbreak involving multiple bacterial strains. J Pediatr. 1976 Dec;89(6):885–891. doi: 10.1016/s0022-3476(76)80591-4. [DOI] [PubMed] [Google Scholar]
  9. Klipstein F. A., Guerrant R. L., Wells J. G., Short H. B., Engert R. F. Comparison of assay of coliform enterotoxins by conventional techniques versus in vivo intestinal perfusion. Infect Immun. 1979 Jul;25(1):146–152. doi: 10.1128/iai.25.1.146-152.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klipstein F. A., Holdeman L. V., Corcino J. J., Moore W. E. Enterotoxigenic intestinal bacteria in tropical sprue. Ann Intern Med. 1973 Nov;79(5):632–641. doi: 10.7326/0003-4819-79-5-632. [DOI] [PubMed] [Google Scholar]
  11. Larson H. E., Price A. B., Honour P., Borriello S. P. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet. 1978 May 20;1(8073):1063–1066. doi: 10.1016/s0140-6736(78)90912-1. [DOI] [PubMed] [Google Scholar]
  12. Lima A. A., Lyerly D. M., Wilkins T. D., Innes D. J., Guerrant R. L. Effects of Clostridium difficile toxins A and B in rabbit small and large intestine in vivo and on cultured cells in vitro. Infect Immun. 1988 Mar;56(3):582–588. doi: 10.1128/iai.56.3.582-588.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lusk R. H., Fekety R., Silva J., Browne R. A., Ringler D. H., Abrams G. D. Clindamycin-induced enterocolitis in hamsters. J Infect Dis. 1978 Apr;137(4):464–475. doi: 10.1093/infdis/137.4.464. [DOI] [PubMed] [Google Scholar]
  14. Lyerly D. M., Lockwood D. E., Richardson S. H., Wilkins T. D. Biological activities of toxins A and B of Clostridium difficile. Infect Immun. 1982 Mar;35(3):1147–1150. doi: 10.1128/iai.35.3.1147-1150.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Minami J., Okabe A., Shiode J., Hayashi H. Production of a unique cytotoxin by Klebsiella oxytoca. Microb Pathog. 1989 Sep;7(3):203–211. doi: 10.1016/0882-4010(89)90056-9. [DOI] [PubMed] [Google Scholar]
  16. Minami J., Saito S., Yoshida T., Uemura T., Okabe A. Biological activities and chemical composition of a cytotoxin of Klebsiella oxytoca. J Gen Microbiol. 1992 Sep;138(9):1921–1927. doi: 10.1099/00221287-138-9-1921. [DOI] [PubMed] [Google Scholar]
  17. Mitchell T. J., Ketley J. M., Haslam S. C., Stephen J., Burdon D. W., Candy D. C., Daniel R. Effect of toxin A and B of Clostridium difficile on rabbit ileum and colon. Gut. 1986 Jan;27(1):78–85. doi: 10.1136/gut.27.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. O'Brien A. D., Holmes R. K. Shiga and Shiga-like toxins. Microbiol Rev. 1987 Jun;51(2):206–220. doi: 10.1128/mr.51.2.206-220.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sakurai Y., Tsuchiya H., Ikegami F., Funatomi T., Takasu S., Uchikoshi T. Acute right-sided hemorrhagic colitis associated with oral administration of ampicillin. Dig Dis Sci. 1979 Dec;24(12):910–915. doi: 10.1007/BF01311944. [DOI] [PubMed] [Google Scholar]
  20. Taylor N. S., Thorne G. M., Bartlett J. G. Comparison of two toxins produced by Clostridium difficile. Infect Immun. 1981 Dec;34(3):1036–1043. doi: 10.1128/iai.34.3.1036-1043.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Toffler R. B., Pingoud E. G., Burrell M. I. Acute colitis related to penicillin and penicillin derivatives. Lancet. 1978 Sep 30;2(8092 Pt 1):707–709. doi: 10.1016/s0140-6736(78)92704-6. [DOI] [PubMed] [Google Scholar]
  22. Totani T. [Acute hemorrhagic enteritis with Klebsiella oxytoca]. Nihon Rinsho. 1978 May;Suppl:1308–1309. [PubMed] [Google Scholar]
  23. Wachsmuth K., Wells J., Shipley P., Ryder R. Heat-labile enterotoxin production in isolates from a shipboard outbreak of human diarrheal illness. Infect Immun. 1979 Jun;24(3):793–797. doi: 10.1128/iai.24.3.793-797.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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