Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1994 Feb;62(2):371–376. doi: 10.1128/iai.62.2.371-376.1994

Differentiation of Pseudomonas aeruginosa pili based on sequence and B-cell epitope analyses.

P A Castric 1, C D Deal 1
PMCID: PMC186117  PMID: 7507890

Abstract

The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping immobilized peptides representing the pilin primary structure, with antipilin monoclonal antibodies revealed several B-cell determinants grouped near the carboxyl terminus of P. aeruginosa 1244 pilin. One determinant was found to reside near the pilin constant region. These determinants were found associated with the pili of 31 of 95 P. aeruginosa clinical isolates.

Full text

PDF
371

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodey G. P., Bolivar R., Fainstein V., Jadeja L. Infections caused by Pseudomonas aeruginosa. Rev Infect Dis. 1983 Mar-Apr;5(2):279–313. doi: 10.1093/clinids/5.2.279. [DOI] [PubMed] [Google Scholar]
  2. Bradley D. E. A function of Pseudomonas aeruginosa PAO polar pili: twitching motility. Can J Microbiol. 1980 Feb;26(2):146–154. doi: 10.1139/m80-022. [DOI] [PubMed] [Google Scholar]
  3. Bradley D. E. Shortening of Pseudomonas aeruginosa pili after RNA-phage adsorption. J Gen Microbiol. 1972 Sep;72(2):303–319. doi: 10.1099/00221287-72-2-303. [DOI] [PubMed] [Google Scholar]
  4. Carter J. M., Vanalbert S., Lee J., Lyon J., Deal C. Shedding light on peptide synthesis. Biotechnology (N Y) 1992 May;10(5):509–513. doi: 10.1038/nbt0592-509. [DOI] [PubMed] [Google Scholar]
  5. Castric P. A., Sidberry H. F., Sadoff J. C. Cloning and sequencing of the Pseudomonas aeruginosa 1244 pilin structural gene. Mol Gen Genet. 1989 Mar;216(1):75–80. doi: 10.1007/BF00332233. [DOI] [PubMed] [Google Scholar]
  6. Dalrymple B., Mattick J. S. An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins. J Mol Evol. 1987;25(3):261–269. doi: 10.1007/BF02100020. [DOI] [PubMed] [Google Scholar]
  7. Doig P., Sastry P. A., Hodges R. S., Lee K. K., Paranchych W., Irvin R. T. Inhibition of pilus-mediated adhesion of Pseudomonas aeruginosa to human buccal epithelial cells by monoclonal antibodies directed against pili. Infect Immun. 1990 Jan;58(1):124–130. doi: 10.1128/iai.58.1.124-130.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Doig P., Todd T., Sastry P. A., Lee K. K., Hodges R. S., Paranchych W., Irvin R. T. Role of pili in adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells. Infect Immun. 1988 Jun;56(6):1641–1646. doi: 10.1128/iai.56.6.1641-1646.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Elleman T. C. Pilins of Bacteroides nodosus: molecular basis of serotypic variation and relationships to other bacterial pilins. Microbiol Rev. 1988 Jun;52(2):233–247. doi: 10.1128/mr.52.2.233-247.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Faast R., Ogierman M. A., Stroeher U. H., Manning P. A. Nucleotide sequence of the structural gene, tcpA, for a major pilin subunit of Vibrio cholerae. Gene. 1989 Dec 21;85(1):227–231. doi: 10.1016/0378-1119(89)90486-1. [DOI] [PubMed] [Google Scholar]
  11. Geysen H. M., Meloen R. H., Barteling S. J. Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc Natl Acad Sci U S A. 1984 Jul;81(13):3998–4002. doi: 10.1073/pnas.81.13.3998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hobbs M., Dalrymple B., Delaney S. F., Mattick J. S. Transcription of the fimbrial subunit gene and an associated transfer RNA gene of Pseudomonas aeruginosa. Gene. 1988;62(2):219–227. doi: 10.1016/0378-1119(88)90560-4. [DOI] [PubMed] [Google Scholar]
  13. Irvin R. T., Doig P., Lee K. K., Sastry P. A., Paranchych W., Todd T., Hodges R. S. Characterization of the Pseudomonas aeruginosa pilus adhesin: confirmation that the pilin structural protein subunit contains a human epithelial cell-binding domain. Infect Immun. 1989 Dec;57(12):3720–3726. doi: 10.1128/iai.57.12.3720-3726.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lee K. K., Doig P., Irvin R. T., Paranchych W., Hodges R. S. Mapping the surface regions of Pseudomonas aeruginosa PAK pilin: the importance of the C-terminal region for adherence to human buccal epithelial cells. Mol Microbiol. 1989 Nov;3(11):1493–1499. doi: 10.1111/j.1365-2958.1989.tb00135.x. [DOI] [PubMed] [Google Scholar]
  15. Lee K. K., Sastry P. A., Paranchych W., Hodges R. S. Immunological studies of the disulfide bridge region of Pseudomonas aeruginosa PAK and PAO pilins, using anti-PAK pilus and antipeptide antibodies. Infect Immun. 1989 Feb;57(2):520–526. doi: 10.1128/iai.57.2.520-526.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marrs C. F., Ruehl W. W., Schoolnik G. K., Falkow S. Pilin-gene phase variation of Moraxella bovis is caused by an inversion of the pilin genes. J Bacteriol. 1988 Jul;170(7):3032–3039. doi: 10.1128/jb.170.7.3032-3039.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Marrs C. F., Schoolnik G., Koomey J. M., Hardy J., Rothbard J., Falkow S. Cloning and sequencing of a Moraxella bovis pilin gene. J Bacteriol. 1985 Jul;163(1):132–139. doi: 10.1128/jb.163.1.132-139.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Meyer T. F., Billyard E., Haas R., Storzbach S., So M. Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6110–6114. doi: 10.1073/pnas.81.19.6110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meyer T. F., Gibbs C. P., Haas R. Variation and control of protein expression in Neisseria. Annu Rev Microbiol. 1990;44:451–477. doi: 10.1146/annurev.mi.44.100190.002315. [DOI] [PubMed] [Google Scholar]
  20. Nunn D., Bergman S., Lory S. Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili. J Bacteriol. 1990 Jun;172(6):2911–2919. doi: 10.1128/jb.172.6.2911-2919.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ottow J. C. Ecology, physiology, and genetics of fimbriae and pili. Annu Rev Microbiol. 1975;29:79–108. doi: 10.1146/annurev.mi.29.100175.000455. [DOI] [PubMed] [Google Scholar]
  22. Paranchych W., Frost L. S. The physiology and biochemistry of pili. Adv Microb Physiol. 1988;29:53–114. doi: 10.1016/s0065-2911(08)60346-x. [DOI] [PubMed] [Google Scholar]
  23. Pasloske B. L., Finlay B. B., Paranchych W. Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene. FEBS Lett. 1985 Apr 22;183(2):408–412. doi: 10.1016/0014-5793(85)80821-8. [DOI] [PubMed] [Google Scholar]
  24. Pasloske B. L., Sastry P. A., Finlay B. B., Paranchych W. Two unusual pilin sequences from different isolates of Pseudomonas aeruginosa. J Bacteriol. 1988 Aug;170(8):3738–3741. doi: 10.1128/jb.170.8.3738-3741.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ramphal R., Sadoff J. C., Pyle M., Silipigni J. D. Role of pili in the adherence of Pseudomonas aeruginosa to injured tracheal epithelium. Infect Immun. 1984 Apr;44(1):38–40. doi: 10.1128/iai.44.1.38-40.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rao V. K., Progulske-Fox A. Cloning and sequencing of two type 4 (N-methylphenylalanine) pilin genes from Eikenella corrodens. J Gen Microbiol. 1993 Mar;139(3):651–660. doi: 10.1099/00221287-139-3-651. [DOI] [PubMed] [Google Scholar]
  27. Saiman L., Sadoff J., Prince A. Cross-reactivity of Pseudomonas aeruginosa antipilin monoclonal antibodies with heterogeneous strains of P. aeruginosa and Pseudomonas cepacia. Infect Immun. 1989 Sep;57(9):2764–2770. doi: 10.1128/iai.57.9.2764-2770.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Watts T. H., Sastry P. A., Hodges R. S., Paranchych W. Mapping of the antigenic determinants of Pseudomonas aeruginosa PAK polar pili. Infect Immun. 1983 Oct;42(1):113–121. doi: 10.1128/iai.42.1.113-121.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weir S., Marrs C. F. Identification of type 4 pili in Kingella denitrificans. Infect Immun. 1992 Aug;60(8):3437–3441. doi: 10.1128/iai.60.8.3437-3441.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. West S. E., Iglewski B. H. Codon usage in Pseudomonas aeruginosa. Nucleic Acids Res. 1988 Oct 11;16(19):9323–9335. doi: 10.1093/nar/16.19.9323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Woods D. E., Straus D. C., Johanson W. G., Jr, Berry V. K., Bass J. A. Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infect Immun. 1980 Sep;29(3):1146–1151. doi: 10.1128/iai.29.3.1146-1151.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES