Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1994 Feb;62(2):726–728. doi: 10.1128/iai.62.2.726-728.1994

The Mycobacterium bovis 32-kilodalton protein antigen induces human cytotoxic T-cell responses.

M E Munk 1, J De Bruyn 1, H Gras 1, S H Kaufmann 1
PMCID: PMC186167  PMID: 8300233

Abstract

The 30-kDa protein (P32) is a mycobacterial secreted antigen which is homologous in Mycobacterium bovis and M. tuberculosis. In vitro, P32 induced T-cell proliferation. M. tuberculosis- or P32-stimulated T-cell lines lysed macrophages pulsed with P32 or M. tuberculosis, respectively. We conclude that P32 stimulates cytotoxic T cells specifically.

Full text

PDF
726

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abou-Zeid C., Smith I., Grange J. M., Ratliff T. L., Steele J., Rook G. A. The secreted antigens of Mycobacterium tuberculosis and their relationship to those recognized by the available antibodies. J Gen Microbiol. 1988 Feb;134(2):531–538. doi: 10.1099/00221287-134-2-531. [DOI] [PubMed] [Google Scholar]
  2. Barnes P. F., Mehra V., Hirschfield G. R., Fong S. J., Abou-Zeid C., Rook G. A., Hunter S. W., Brennan P. J., Modlin R. L. Characterization of T cell antigens associated with the cell wall protein-peptidoglycan complex of Mycobacterium tuberculosis. J Immunol. 1989 Oct 15;143(8):2656–2662. [PubMed] [Google Scholar]
  3. Borremans M., de Wit L., Volckaert G., Ooms J., de Bruyn J., Huygen K., van Vooren J. P., Stelandre M., Verhofstadt R., Content J. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis. Infect Immun. 1989 Oct;57(10):3123–3130. doi: 10.1128/iai.57.10.3123-3130.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Content J., de la Cuvellerie A., De Wit L., Vincent-Levy-Frébault V., Ooms J., De Bruyn J. The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis. Infect Immun. 1991 Sep;59(9):3205–3212. doi: 10.1128/iai.59.9.3205-3212.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Bruyn J., Huygen K., Bosmans R., Fauville M., Lippens R., Van Vooren J. P., Falmagne P., Weckx M., Wiker H. G., Harboe M. Purification, characterization and identification of a 32 kDa protein antigen of Mycobacterium bovis BCG. Microb Pathog. 1987 May;2(5):351–366. doi: 10.1016/0882-4010(87)90077-5. [DOI] [PubMed] [Google Scholar]
  6. De Wit L., de la Cuvellerie A., Ooms J., Content J. Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG. Nucleic Acids Res. 1990 Jul 11;18(13):3995–3995. doi: 10.1093/nar/18.13.3995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huygen K., Van Vooren J. P., Turneer M., Bosmans R., Dierckx P., De Bruyn J. Specific lymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis. Scand J Immunol. 1988 Feb;27(2):187–194. doi: 10.1111/j.1365-3083.1988.tb02338.x. [DOI] [PubMed] [Google Scholar]
  8. Kaufmann S. H. Immunity to intracellular bacteria. Annu Rev Immunol. 1993;11:129–163. doi: 10.1146/annurev.iy.11.040193.001021. [DOI] [PubMed] [Google Scholar]
  9. Launois P., Huygen K., De Bruyn J., N'Diaye M., Diouf B., Sarthouj L., Grimaud J., Millan J. T cell response to purified filtrate antigen 85 from Mycobacterium bovis Bacilli Calmette-Guérin (BCG) in leprosy patients. Clin Exp Immunol. 1991 Nov;86(2):286–290. doi: 10.1111/j.1365-2249.1991.tb05811.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Launois P., Niang M. N., De Bruyn J., Sarthou J. L., Rivier F., Drowart A., Van Vooren J. P., Millan J., Huygen K. The major secreted antigen complex (Ag 85) from Mycobacterium bovis bacille Calmette-Guérin is associated with protective T cells in leprosy: a follow-up study of 45 household contacts. J Infect Dis. 1993 May;167(5):1160–1167. doi: 10.1093/infdis/167.5.1160. [DOI] [PubMed] [Google Scholar]
  11. Lima L. de M., Content J., van Heuverswyn H., Degrave W. Nucleotide sequence of the gene coding for the 85-B antigen of Mycobacterium leprae. Nucleic Acids Res. 1991 Oct 25;19(20):5789–5789. doi: 10.1093/nar/19.20.5789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Manca F., Rossi G., Valle M. T., Lantero S., Li Pira G., Fenoglio D., De Bruin J., Costantini M., Damiani G., Balbi B. Limited clonal heterogeneity of antigen-specific T cells localizing in the pleural space during mycobacterial infection. Infect Immun. 1991 Feb;59(2):503–513. doi: 10.1128/iai.59.2.503-513.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matsuo K., Yamaguchi R., Yamazaki A., Tasaka H., Terasaka K., Yamada T. Cloning and expression of the gene for the cross-reactive alpha antigen of Mycobacterium kansasii. Infect Immun. 1990 Feb;58(2):550–556. doi: 10.1128/iai.58.2.550-556.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Matsuo K., Yamaguchi R., Yamazaki A., Tasaka H., Yamada T. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. J Bacteriol. 1988 Sep;170(9):3847–3854. doi: 10.1128/jb.170.9.3847-3854.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Munk M. E., Gatrill A. J., Kaufmann S. H. Target cell lysis and IL-2 secretion by gamma/delta T lymphocytes after activation with bacteria. J Immunol. 1990 Oct 15;145(8):2434–2439. [PubMed] [Google Scholar]
  16. Munk M. E., Schoel B., Modrow S., Karr R. W., Young R. A., Kaufmann S. H. T lymphocytes from healthy individuals with specificity to self-epitopes shared by the mycobacterial and human 65-kilodalton heat shock protein. J Immunol. 1989 Nov 1;143(9):2844–2849. [PubMed] [Google Scholar]
  17. Ottenhoff T. H., Ab B. K., Van Embden J. D., Thole J. E., Kiessling R. The recombinant 65-kD heat shock protein of Mycobacterium bovis Bacillus Calmette-Guerin/M. tuberculosis is a target molecule for CD4+ cytotoxic T lymphocytes that lyse human monocytes. J Exp Med. 1988 Nov 1;168(5):1947–1952. doi: 10.1084/jem.168.5.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pancholi P., Mirza A., Bhardwaj N., Steinman R. M. Sequestration from immune CD4+ T cells of mycobacteria growing in human macrophages. Science. 1993 May 14;260(5110):984–986. doi: 10.1126/science.8098550. [DOI] [PubMed] [Google Scholar]
  19. Rambukkana A., Burggraaf J. D., Faber W. R., Harboe M., Teeling P., Krieg S., Das P. K. The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues. Infect Immun. 1993 May;61(5):1835–1845. doi: 10.1128/iai.61.5.1835-1845.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rambukkana A., Das P. K., Burggraaf J. D., Faber W. R., Teeling P., Krieg S., Thole J. E., Harboe M. Identification and characterization of epitopes shared between the mycobacterial 65-kilodalton heat shock protein and the actively secreted antigen 85 complex: their in situ expression on the cell wall surface of Mycobacterium leprae. Infect Immun. 1992 Nov;60(11):4517–4527. doi: 10.1128/iai.60.11.4517-4527.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rambukkana A., Das P. K., Burggraaf J. D., Yong S., Faber W. R., Thole J. E., Harboe M. Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface. Infect Immun. 1992 Dec;60(12):5172–5181. doi: 10.1128/iai.60.12.5172-5181.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thole J. E., Schöningh R., Janson A. A., Garbe T., Cornelisse Y. E., Clark-Curtiss J. E., Kolk A. H., Ottenhoff T. H., De Vries R. R., Abou-Zeid C. Molecular and immunological analysis of a fibronectin-binding protein antigen secreted by Mycobacterium leprae. Mol Microbiol. 1992 Jan;6(2):153–163. doi: 10.1111/j.1365-2958.1992.tb01996.x. [DOI] [PubMed] [Google Scholar]
  23. Turneer M., Van Vooren J. P., De Bruyn J., Serruys E., Dierckx P., Yernault J. C. Humoral immune response in human tuberculosis: immunoglobulins G, A, and M directed against the purified P32 protein antigen of Mycobacterium bovis bacillus Calmette-Guérin. J Clin Microbiol. 1988 Sep;26(9):1714–1719. doi: 10.1128/jcm.26.9.1714-1719.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wiker H. G., Harboe M., Nagai S., Patarroyo M. E., Ramirez C., Cruz N. MPB59, a widely cross-reacting protein of Mycobacterium bovis BCG. Int Arch Allergy Appl Immunol. 1986;81(4):307–314. doi: 10.1159/000234154. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES