Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1994 Apr;62(4):1474–1477. doi: 10.1128/iai.62.4.1474-1477.1994

Effect of spleen cell populations on resolution of Cryptosporidium parvum infection in SCID mice.

L E Perryman 1, P H Mason 1, C E Chrisp 1
PMCID: PMC186306  PMID: 7907581

Abstract

Persistent infection was established in SCID mice given 10(7) Cryptosporidium parvum oocysts. Nine groups of infected SCID mice were inoculated with 10(6), 10(5), or 10(4) total spleen cells, CD8-depleted spleen cells, or CD4-depleted spleen cells from naive BALB/c donors. Infection was significantly reduced in all treatment groups. The most profound effect occurred with spleen cell preparations containing CD4 T lymphocytes but depleted of CD8 T lymphocytes.

Full text

PDF
1474

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguirre S. A., Mason P. H., Perryman L. E. Susceptibility of major histocompatibility complex (MHC) class I- and MHC class II-deficient mice to Cryptosporidium parvum infection. Infect Immun. 1994 Feb;62(2):697–699. doi: 10.1128/iai.62.2.697-699.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andreani T., Modigliani R., le Charpentier Y., Galian A., Brouet J. C., Liance M., Lachance J. R., Messing B., Vernisse B. Acquired immunodeficiency with intestinal cryptosporidiosis: possible transmission by Haitian whole blood. Lancet. 1983 May 28;1(8335):1187–1191. doi: 10.1016/s0140-6736(83)92466-2. [DOI] [PubMed] [Google Scholar]
  3. Bjorneby J. M., Hunsaker B. D., Riggs M. W., Perryman L. E. Monoclonal antibody immunotherapy in nude mice persistently infected with Cryptosporidium parvum. Infect Immun. 1991 Mar;59(3):1172–1176. doi: 10.1128/iai.59.3.1172-1176.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bjorneby J. M., Leach D. R., Perryman L. E. Persistent cryptosporidiosis in horses with severe combined immunodeficiency. Infect Immun. 1991 Oct;59(10):3823–3826. doi: 10.1128/iai.59.10.3823-3826.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bosma G. C., Custer R. P., Bosma M. J. A severe combined immunodeficiency mutation in the mouse. Nature. 1983 Feb 10;301(5900):527–530. doi: 10.1038/301527a0. [DOI] [PubMed] [Google Scholar]
  6. Chen W., Harp J. A., Harmsen A. G., Havell E. A. Gamma interferon functions in resistance to Cryptosporidium parvum infection in severe combined immunodeficient mice. Infect Immun. 1993 Aug;61(8):3548–3551. doi: 10.1128/iai.61.8.3548-3551.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chen W., Harp J. A., Harmsen A. G. Requirements for CD4+ cells and gamma interferon in resolution of established Cryptosporidium parvum infection in mice. Infect Immun. 1993 Sep;61(9):3928–3932. doi: 10.1128/iai.61.9.3928-3932.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coffman R. L. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 1982;69:5–23. doi: 10.1111/j.1600-065x.1983.tb00446.x. [DOI] [PubMed] [Google Scholar]
  9. Current W. L., Bick P. H. Immunobiology of Cryptosporidium spp. Pathol Immunopathol Res. 1989;8(3-4):141–160. doi: 10.1159/000157146. [DOI] [PubMed] [Google Scholar]
  10. Current W. L., Reese N. C., Ernst J. V., Bailey W. S., Heyman M. B., Weinstein W. M. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission. N Engl J Med. 1983 May 26;308(21):1252–1257. doi: 10.1056/NEJM198305263082102. [DOI] [PubMed] [Google Scholar]
  11. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintáns J., Loken M. R., Pierres M., Fitch F. W. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445–2451. [PubMed] [Google Scholar]
  12. Fayer R., Ungar B. L. Cryptosporidium spp. and cryptosporidiosis. Microbiol Rev. 1986 Dec;50(4):458–483. doi: 10.1128/mr.50.4.458-483.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Garcia L. S., Current W. L. Cryptosporidiosis: clinical features and diagnosis. Crit Rev Clin Lab Sci. 1989;27(6):439–460. doi: 10.3109/10408368909114594. [DOI] [PubMed] [Google Scholar]
  14. Grusby M. J., Johnson R. S., Papaioannou V. E., Glimcher L. H. Depletion of CD4+ T cells in major histocompatibility complex class II-deficient mice. Science. 1991 Sep 20;253(5026):1417–1420. doi: 10.1126/science.1910207. [DOI] [PubMed] [Google Scholar]
  15. Heine J., Moon H. W., Woodmansee D. B. Persistent Cryptosporidium infection in congenitally athymic (nude) mice. Infect Immun. 1984 Mar;43(3):856–859. doi: 10.1128/iai.43.3.856-859.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koller B. H., Marrack P., Kappler J. W., Smithies O. Normal development of mice deficient in beta 2M, MHC class I proteins, and CD8+ T cells. Science. 1990 Jun 8;248(4960):1227–1230. doi: 10.1126/science.2112266. [DOI] [PubMed] [Google Scholar]
  17. Kuhls T. L., Greenfield R. A., Mosier D. A., Crawford D. L., Joyce W. A. Cryptosporidiosis in adult and neonatal mice with severe combined immunodeficiency. J Comp Pathol. 1992 May;106(4):399–410. doi: 10.1016/0021-9975(92)90024-o. [DOI] [PubMed] [Google Scholar]
  18. Lauzon R. J., Siminovitch K. A., Fulop G. M., Phillips R. A., Roder J. C. An expanded population of natural killer cells in mice with severe combined immunodeficiency (SCID) lack rearrangement and expression of T cell receptor genes. J Exp Med. 1986 Nov 1;164(5):1797–1802. doi: 10.1084/jem.164.5.1797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Magnuson N. S., Perryman L. E., Wyatt C. R., Mason P. H., Talmadge J. E. Large granular lymphocytes from SCID horses develop potent cytotoxic activity after treatment with human recombinant interleukin 2. J Immunol. 1987 Jul 1;139(1):61–67. [PubMed] [Google Scholar]
  20. McDonald V., Deer R., Uni S., Iseki M., Bancroft G. J. Immune responses to Cryptosporidium muris and Cryptosporidium parvum in adult immunocompetent or immunocompromised (nude and SCID) mice. Infect Immun. 1992 Aug;60(8):3325–3331. doi: 10.1128/iai.60.8.3325-3331.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McGuire T. C., Poppie M. J., Banks K. L. Combined (B- and T-lymphocyte) immunodeficiency: a fatal genetic disease in Arabian foals. J Am Vet Med Assoc. 1974 Jan 1;164(1):70–76. [PubMed] [Google Scholar]
  22. Mead J. R., Arrowood M. J., Healey M. C., Sidwell R. W. Cryptosporidial infections in SCID mice reconstituted with human or murine lymphocytes. J Protozool. 1991 Nov-Dec;38(6):59S–61S. [PubMed] [Google Scholar]
  23. Mead J. R., Arrowood M. J., Sidwell R. W., Healey M. C. Chronic Cryptosporidium parvum infections in congenitally immunodeficient SCID and nude mice. J Infect Dis. 1991 Jun;163(6):1297–1304. doi: 10.1093/infdis/163.6.1297. [DOI] [PubMed] [Google Scholar]
  24. Perryman L. E., Kegerris K. A., Mason P. H. Effect of orally administered monoclonal antibody on persistent Cryptosporidium parvum infection in scid mice. Infect Immun. 1993 Nov;61(11):4906–4908. doi: 10.1128/iai.61.11.4906-4908.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pitlik S. D., Fainstein V., Garza D., Guarda L., Bolivar R., Rios A., Hopfer R. L., Mansell P. A. Human cryptosporidiosis: spectrum of disease. Report of six cases and review of the literature. Arch Intern Med. 1983 Dec;143(12):2269–2275. doi: 10.1001/archinte.1983.00350120059015. [DOI] [PubMed] [Google Scholar]
  26. Reimann J., Rudolphi A., Claesson M. H. Selective reconstitution of T lymphocyte subsets in scid mice. Immunol Rev. 1991 Dec;124:75–95. doi: 10.1111/j.1600-065x.1991.tb00617.x. [DOI] [PubMed] [Google Scholar]
  27. Riggs M. W., Perryman L. E. Infectivity and neutralization of Cryptosporidium parvum sporozoites. Infect Immun. 1987 Sep;55(9):2081–2087. doi: 10.1128/iai.55.9.2081-2087.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Rohlman V. C., Kuhls T. L., Mosier D. A., Crawford D. L., Greenfield R. A. Cryptosporidium parvum infection after abrogation of natural killer cell activity in normal and severe combined immunodeficiency mice. J Parasitol. 1993 Apr;79(2):295–297. [PubMed] [Google Scholar]
  29. Sarmiento M., Glasebrook A. L., Fitch F. W. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement. J Immunol. 1980 Dec;125(6):2665–2672. [PubMed] [Google Scholar]
  30. Springer T., Galfrè G., Secher D. S., Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539–551. doi: 10.1002/eji.1830080802. [DOI] [PubMed] [Google Scholar]
  31. Ungar B. L., Burris J. A., Quinn C. A., Finkelman F. D. New mouse models for chronic Cryptosporidium infection in immunodeficient hosts. Infect Immun. 1990 Apr;58(4):961–969. doi: 10.1128/iai.58.4.961-969.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Ungar B. L., Kao T. C., Burris J. A., Finkelman F. D. Cryptosporidium infection in an adult mouse model. Independent roles for IFN-gamma and CD4+ T lymphocytes in protective immunity. J Immunol. 1991 Aug 1;147(3):1014–1022. [PubMed] [Google Scholar]
  33. Wyatt C. R., Magnuson N. S., Perryman L. E. Defective thymocyte maturation in horses with severe combined immunodeficiency. J Immunol. 1987 Dec 15;139(12):4072–4076. [PubMed] [Google Scholar]
  34. Zijlstra M., Bix M., Simister N. E., Loring J. M., Raulet D. H., Jaenisch R. Beta 2-microglobulin deficient mice lack CD4-8+ cytolytic T cells. Nature. 1990 Apr 19;344(6268):742–746. doi: 10.1038/344742a0. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES