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. 1994 May;62(5):2098–2100. doi: 10.1128/iai.62.5.2098-2100.1994

Monoclonal antibodies to the circumsporozoite protein repeats of a Plasmodium vivax-like human malaria parasite and Plasmodium simiovale.

V Udhayakumar 1, S H Qari 1, P Patterson 1, W E Collins 1, A A Lal 1
PMCID: PMC186472  PMID: 8168975

Abstract

We have recently described a Plasmodium vivax-like human malaria parasite. The circumsporozoite protein of this parasite is identical to that of a simian malaria parasite, P. simiovale, but different from two known types of P. vivax. Here, we describe the production of two monoclonal antibodies, Pam 172 and Pam 135, specific for the circumsporozoite protein repeat sequence APGANQEGGAA of the P. vivax-like malaria parasite. These two monoclonal antibodies recognized air-dried sporozoites of P. simiovale but not other human, simian, or rodent malaria parasites tested.

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Selected References

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  1. Arnot D. E., Barnwell J. W., Tam J. P., Nussenzweig V., Nussenzweig R. S., Enea V. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science. 1985 Nov 15;230(4727):815–818. doi: 10.1126/science.2414847. [DOI] [PubMed] [Google Scholar]
  2. Charoenvit Y., Collins W. E., Jones T. R., Millet P., Yuan L., Campbell G. H., Beaudoin R. L., Broderson J. R., Hoffman S. L. Inability of malaria vaccine to induce antibodies to a protective epitope within its sequence. Science. 1991 Feb 8;251(4994):668–671. doi: 10.1126/science.1704150. [DOI] [PubMed] [Google Scholar]
  3. Cochrane A. H., Barnwell J. W., Collins W. E., Nussenzweig R. S. Monoclonal antibodies produced against sporozoites of the human parasite Plasmodium malariae abolish infectivity of sporozoites of the simian parasite Plasmodium brasilianum. Infect Immun. 1985 Oct;50(1):58–61. doi: 10.1128/iai.50.1.58-61.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DISSANAIKE A. S. SIMIAN MALARIA PARASITES OF CEYLON. Bull World Health Organ. 1965;32:593–597. [PMC free article] [PubMed] [Google Scholar]
  5. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  6. McCutchan T. F., Lal A. A., de la Cruz V. F., Miller L. H., Maloy W. L., Charoenvit Y., Beaudoin R. L., Guerry P., Wistar R., Jr, Hoffman S. L. Sequence of the immunodominant epitope for the surface protein on sporozoites of Plasmodium vivax. Science. 1985 Dec 20;230(4732):1381–1383. doi: 10.1126/science.2416057. [DOI] [PubMed] [Google Scholar]
  7. Procell P. M., Collins W. E., Campbell G. H. Circumsporozoite protein of the human malaria parasite Plasmodium ovale identified with monoclonal antibodies. Infect Immun. 1988 Feb;56(2):376–379. doi: 10.1128/iai.56.2.376-379.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Qari S. H., Shi Y. P., Goldman I. F., Udhayakumar V., Alpers M. P., Collins W. E., Lal A. A. Identification of Plasmodium vivax-like human malaria parasite. Lancet. 1993 Mar 27;341(8848):780–783. doi: 10.1016/0140-6736(93)90559-y. [DOI] [PubMed] [Google Scholar]
  9. Qari S. H., Shi Y. P., Povoa M. M., Alpers M. P., Deloron P., Murphy G. S., Harjosuwarno S., Lal A. A. Global occurrence of Plasmodium vivax-like human malaria parasite. J Infect Dis. 1993 Dec;168(6):1485–1489. doi: 10.1093/infdis/168.6.1485. [DOI] [PubMed] [Google Scholar]
  10. Rosenberg R., Wirtz R. A., Lanar D. E., Sattabongkot J., Hall T., Waters A. P., Prasittisuk C. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science. 1989 Sep 1;245(4921):973–976. doi: 10.1126/science.2672336. [DOI] [PubMed] [Google Scholar]
  11. Wirtz R. A., Burkot T. R., Andre R. G., Rosenberg R., Collins W. E., Roberts D. R. Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 1985 Nov;34(6):1048–1054. doi: 10.4269/ajtmh.1985.34.1048. [DOI] [PubMed] [Google Scholar]
  12. Yoshida N., Nussenzweig R. S., Potocnjak P., Nussenzweig V., Aikawa M. Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science. 1980 Jan 4;207(4426):71–73. doi: 10.1126/science.6985745. [DOI] [PubMed] [Google Scholar]
  13. Zavala F., Gwadz R. W., Collins F. H., Nussenzweig R. S., Nussenzweig V. Monoclonal antibodies to circumsporozoite proteins identify the species of malaria parasite in infected mosquitoes. Nature. 1982 Oct 21;299(5885):737–738. doi: 10.1038/299737a0. [DOI] [PubMed] [Google Scholar]
  14. Zegers N., Gerritse K., Deen C., Boersma W., Claassen E. An improved conjugation method for controlled covalent coupling of synthetic peptides to proteins using glutaraldehyde in a dialysis method. J Immunol Methods. 1990 Jul 3;130(2):195–200. doi: 10.1016/0022-1759(90)90048-z. [DOI] [PubMed] [Google Scholar]

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