Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1994 Jun;62(6):2426–2431. doi: 10.1128/iai.62.6.2426-2431.1994

Comparison of nonhuman primate antibodies against Haemophilus influenzae type b polysaccharide with human antibodies in oligoclonality and in vivo protective potency.

K H Kim 1, M K Park 1, C C Peeters 1, J T Poolman 1, M H Shearer 1, R C Kennedy 1, M H Nahm 1
PMCID: PMC186528  PMID: 8188367

Abstract

Nonhuman primates are often used as a model for studying vaccines for humans. However, it is not always clear how closely the antibody responses in these species mimic human responses. Recent studies have characterized the human antibody response to Haemophilus influenzae type b (Hib) in great detail. In this study, we have compared the antibody response to Hib of humans with those of other primates. Studies of isoelectric points and V kappa subgroup usage show that, like humans, nonhuman primates produce oligoclonal antibodies. Also, monkey antibodies to the Hib polysaccharide are as protective as human antibodies in an in vivo model of Hib infection. Thus, we conclude that nonhuman primates produce antibodies to Hib polysaccharide that are structurally and functionally similar to human antibodies and are a good model for testing human vaccines.

Full text

PDF
2426

Images in this article

2428Image
on p.2428

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adderson E. E., Shackelford P. G., Insel R. A., Quinn A., Wilson P. M., Carroll W. L. Immunoglobulin light chain variable region gene sequences for human antibodies to Haemophilus influenzae type b capsular polysaccharide are dominated by a limited number of V kappa and V lambda segments and VJ combinations. J Clin Invest. 1992 Mar;89(3):729–738. doi: 10.1172/JCI115649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adderson E. E., Shackelford P. G., Quinn A., Carroll W. L. Restricted Ig H chain V gene usage in the human antibody response to Haemophilus influenzae type b capsular polysaccharide. J Immunol. 1991 Sep 1;147(5):1667–1674. [PubMed] [Google Scholar]
  3. Amir J., Liang X., Granoff D. M. Variability in the functional activity of vaccine-induced antibody to Haemophilus influenzae type b. Pediatr Res. 1990 Apr;27(4 Pt 1):358–364. doi: 10.1203/00006450-199004000-00008. [DOI] [PubMed] [Google Scholar]
  4. Amir J., Scott M. G., Nahm M. H., Granoff D. M. Bactericidal and opsonic activity of IgG1 and IgG2 anticapsular antibodies to Haemophilus influenzae type b. J Infect Dis. 1990 Jul;162(1):163–171. doi: 10.1093/infdis/162.1.163. [DOI] [PubMed] [Google Scholar]
  5. Axelrod O., Silverman G. J., Dev V., Kyle R., Carson D. A., Kipps T. J. Idiotypic cross-reactivity of immunoglobulins expressed in Waldenström's macroglobulinemia, chronic lymphocytic leukemia, and mantle zone lymphocytes of secondary B-cell follicles. Blood. 1991 Apr 1;77(7):1484–1490. [PubMed] [Google Scholar]
  6. Balayan M. S. Natural hosts of hepatitis A virus. Vaccine. 1992;10 (Suppl 1):S27–S31. doi: 10.1016/0264-410x(92)90537-t. [DOI] [PubMed] [Google Scholar]
  7. Chung G. H., Scott M. G., Kim K. H., Kearney J., Siber G. R., Ambrosino D. M., Nahm M. H. Clonal characterization of the human IgG antibody repertoire to Haemophilus influenzae type b polysaccharide. V. In vivo expression of individual antibody clones is dependent on Ig CH haplotypes and the categories of antigen. J Immunol. 1993 Oct 15;151(8):4352–4361. [PubMed] [Google Scholar]
  8. Cooper L. J., Schimenti J. C., Glass D. D., Greenspan N. S. H chain C domains influence the strength of binding of IgG for streptococcal group A carbohydrate. J Immunol. 1991 Apr 15;146(8):2659–2663. [PubMed] [Google Scholar]
  9. Dolan K. T., Staub J. M., Schofield T. L., Ahonkhai V. I., Ellis R. W., Vella P. P. An enzyme-linked immunosorbent assay for quantitation of Haemophilus Influenzae type b polysaccharide-specific IgG1 and IgG2 in human and infant rhesus monkey sera. J Immunoassay. 1991;12(4):543–564. doi: 10.1080/01971529108053279. [DOI] [PubMed] [Google Scholar]
  10. Insel R. A., Kittelberger A., Anderson P. Isoelectric focusing of human antibody to the Haemophilus influenzae b capsular polysaccharide: restricted and identical spectrotypes in adults. J Immunol. 1985 Oct;135(4):2810–2816. [PubMed] [Google Scholar]
  11. Kroemer G., Helmberg A., Bernot A., Auffray C., Kofler R. Evolutionary relationship between human and mouse immunoglobulin kappa light chain variable region genes. Immunogenetics. 1991;33(1):42–49. doi: 10.1007/BF00211694. [DOI] [PubMed] [Google Scholar]
  12. Letvin N. L. Nonhuman primate models for HIV vaccine development. Immunodefic Rev. 1992;3(3):247–260. [PubMed] [Google Scholar]
  13. Lucas A. H., Langley R. J., Granoff D. M., Nahm M. H., Kitamura M. Y., Scott M. G. An idiotypic marker associated with a germ-line encoded kappa light chain variable region that predominates the vaccine-induced human antibody response to the Haemophilus influenzae b polysaccharide. J Clin Invest. 1991 Dec;88(6):1811–1818. doi: 10.1172/JCI115502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mageed R. A., Walker M. R., Jefferis R. Restricted light chain subgroup expression on human rheumatoid factor paraproteins determined by monoclonal antibodies. Immunology. 1986 Nov;59(3):473–478. [PMC free article] [PubMed] [Google Scholar]
  15. Mäkelä O., Mattila P., Rautonen N., Seppälä I., Eskola J., Käyhty H. Isotype concentrations of human antibodies to Haemophilus influenzae type b polysaccharide (Hib) in young adults immunized with the polysaccharide as such or conjugated to a protein (diphtheria toxoid). J Immunol. 1987 Sep 15;139(6):1999–2004. [PubMed] [Google Scholar]
  16. Nahm M. H., Clevinger B. L., Davie J. M. Monoclonal antibodies to streptococcal group A carbohydrate. I. A dominant idiotypic determinant is located on Vk. J Immunol. 1982 Oct;129(4):1513–1518. [PubMed] [Google Scholar]
  17. Peeters C. C., Evenberg D., Hoogerhout P., Käyhty H., Saarinen L., van Boeckel C. A., van der Marel G. A., van Boom J. H., Poolman J. T. Synthetic trimer and tetramer of 3-beta-D-ribose-(1-1)-D-ribitol-5-phosphate conjugated to protein induce antibody responses to Haemophilus influenzae type b capsular polysaccharide in mice and monkeys. Infect Immun. 1992 May;60(5):1826–1833. doi: 10.1128/iai.60.5.1826-1833.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Phipps D. C., West J., Eby R., Koster M., Madore D. V., Quataert S. A. An ELISA employing a Haemophilus influenzae type b oligosaccharide-human serum albumin conjugate correlates with the radioantigen binding assay. J Immunol Methods. 1990 Dec 31;135(1-2):121–128. doi: 10.1016/0022-1759(90)90264-v. [DOI] [PubMed] [Google Scholar]
  19. Redl H., Schlag G., Schiesser A., Davies J. Tumor necrosis factor is a mediator of phospholipase release during bacteremia in baboons. Am J Physiol. 1993 Jun;264(6 Pt 2):H2119–H2123. doi: 10.1152/ajpheart.1993.264.6.H2119. [DOI] [PubMed] [Google Scholar]
  20. Reimann K. A., Burkly L. C., Burrus B., Waite B. C., Lord C. I., Letvin N. L. In vivo administration to rhesus monkeys of a CD4-specific monoclonal antibody capable of blocking AIDS virus replication. AIDS Res Hum Retroviruses. 1993 Mar;9(3):199–207. doi: 10.1089/aid.1993.9.199. [DOI] [PubMed] [Google Scholar]
  21. Scott M. G., Nahm M. H. Characterization of the human IgG antibody VL repertoire to Haemophilus influenzae type b polysaccharide. J Infect Dis. 1992 Jun;165 (Suppl 1):S53–S56. doi: 10.1093/infdis/165-supplement_1-s53. [DOI] [PubMed] [Google Scholar]
  22. Scott M. G., Tarrand J. J., Crimmins D. L., McCourt D. W., Siegel N. R., Smith C. E., Nahm M. H. Clonal characterization of the human IgG antibody repertoire to Haemophilus influenzae type b polysaccharide. II. IgG antibodies contain VH genes from a single VH family and VL genes from at least four VL families. J Immunol. 1989 Jul 1;143(1):293–298. [PubMed] [Google Scholar]
  23. Shackelford P. G., Granoff D. M. IgG subclass composition of the antibody response of healthy adults, and normal or IgG2-deficient children to immunization with H. influenzae type b polysaccharide vaccine or Hib PS-protein conjugate vaccines. Monogr Allergy. 1988;23:269–281. [PubMed] [Google Scholar]
  24. Shackelford P. G., Granoff D. M., Nelson S. J., Scott M. G., Smith D. S., Nahm M. H. Subclass distribution of human antibodies to Haemophilus influenzae type b capsular polysaccharide. J Immunol. 1987 Jan 15;138(2):587–592. [PubMed] [Google Scholar]
  25. Tarrand J. J., Scott M. G., Takes P. A., Nahm M. H. Clonal characterization of the human IgG antibody repertoire to Haemophilus influenzae type B polysaccharide. Demonstration of three types of V regions and their association with H and L chain isotypes. J Immunol. 1989 Apr 1;142(7):2519–2526. [PubMed] [Google Scholar]
  26. Vella P. P., Staub J. M., Armstrong J., Dolan K. T., Rusk C. M., Szymanski S., Greer W. E., Marburg S., Kniskern P. J., Schofield T. L. Immunogenicity of a new Haemophilus influenzae type b conjugate vaccine (meningococcal protein conjugate) (PedvaxHIB). Pediatrics. 1990 Apr;85(4 Pt 2):668–675. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES