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. 1994 Jun;62(6):2619–2621. doi: 10.1128/iai.62.6.2619-2621.1994

Infection of human endothelial cells by Rickettsia rickettsii causes a significant reduction in the levels of key enzymes involved in protection against oxidative injury.

P S Devamanoharan 1, L A Santucci 1, J E Hong 1, X Tian 1, D J Silverman 1
PMCID: PMC186554  PMID: 8188386

Abstract

The activities of glucose-6-phosphate dehydrogenase, catalase, and glutathione peroxidase were significantly decreased in human endothelial cells infected with the obligate intracellular bacterium Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever. This observation lends additional support to our hypothesis implicating oxidative damage in endothelial cell injury caused by this microorganism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEERS R. F., Jr, SIZER I. W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133–140. [PubMed] [Google Scholar]
  2. Blum J., Fridovich I. Inactivation of glutathione peroxidase by superoxide radical. Arch Biochem Biophys. 1985 Aug 1;240(2):500–508. doi: 10.1016/0003-9861(85)90056-6. [DOI] [PubMed] [Google Scholar]
  3. GIMENEZ D. F. STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964 May;39:135–140. doi: 10.3109/10520296409061219. [DOI] [PubMed] [Google Scholar]
  4. Günzler W. A., Kremers H., Flohé L. An improved coupled test procedure for glutathione peroxidase (EC 1-11-1-9-) in blood. Z Klin Chem Klin Biochem. 1974 Oct;12(10):444–448. doi: 10.1515/cclm.1974.12.10.444. [DOI] [PubMed] [Google Scholar]
  5. Jornot L., Junod A. F. Response of human endothelial cell antioxidant enzymes to hyperoxia. Am J Respir Cell Mol Biol. 1992 Jan;6(1):107–115. doi: 10.1165/ajrcmb/6.1.107. [DOI] [PubMed] [Google Scholar]
  6. Kono Y., Fridovich I. Superoxide radical inhibits catalase. J Biol Chem. 1982 May 25;257(10):5751–5754. [PubMed] [Google Scholar]
  7. Oliver C. N., Levine R. L., Stadtman E. R. A role of mixed-function oxidation reactions in the accumulation of altered enzyme forms during aging. J Am Geriatr Soc. 1987 Oct;35(10):947–956. doi: 10.1111/j.1532-5415.1987.tb02297.x. [DOI] [PubMed] [Google Scholar]
  8. Royall J. A., Gwin P. D., Parks D. A., Freeman B. A. Responses of vascular endothelial oxidant metabolism to lipopolysaccharide and tumor necrosis factor-alpha. Arch Biochem Biophys. 1992 May 1;294(2):686–694. doi: 10.1016/0003-9861(92)90742-f. [DOI] [PubMed] [Google Scholar]
  9. Santucci L. A., Gutierrez P. L., Silverman D. J. Rickettsia rickettsii induces superoxide radical and superoxide dismutase in human endothelial cells. Infect Immun. 1992 Dec;60(12):5113–5118. doi: 10.1128/iai.60.12.5113-5118.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shingu M., Yoshioka K., Nobunaga M., Yoshida K. Human vascular smooth muscle cells and endothelial cells lack catalase activity and are susceptible to hydrogen peroxide. Inflammation. 1985 Sep;9(3):309–320. doi: 10.1007/BF00916279. [DOI] [PubMed] [Google Scholar]
  11. Silverman D. J. Rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro. Infect Immun. 1984 Jun;44(3):545–553. doi: 10.1128/iai.44.3.545-553.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Silverman D. J., Santucci L. A. A potential protective role for thiols against cell injury caused by Rickettsia rickettsii. Ann N Y Acad Sci. 1990;590:111–117. doi: 10.1111/j.1749-6632.1990.tb42213.x. [DOI] [PubMed] [Google Scholar]
  13. Silverman D. J., Santucci L. A. Potential for free radical-induced lipid peroxidation as a cause of endothelial cell injury in Rocky Mountain spotted fever. Infect Immun. 1988 Dec;56(12):3110–3115. doi: 10.1128/iai.56.12.3110-3115.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]

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