Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1974 Jul;28(1):5–10. doi: 10.1128/am.28.1.5-10.1974

Occurrence of a Complex Form of Staphylokinase in the Course of Cultivation of Staphylococcus aureus

Setsuo Fujimura 1, Toshikazu Makino 1, Takayoshi T A Hayashi 1
PMCID: PMC186571  PMID: 4844264

Abstract

Proteins in the culture supernatant of Staphylococcus aureus PS 47 were subjected to Sephadex chromatography. In the early stage of the cultivation, staphylokinase appeared to have a molecular weight of 15,000 and in the later stage it appeared to have a molecular weight of 320,000. The staphylokinase having a lower molecular weight (type A) converted into one having a higher value (type B) during the course of cultivation. It was demonstrated that conversion of type A into type B took place in vitro (monitored by gel filtration after the two types of staphylokinases were mixed), and it was observed that type B reverted to type A when type B was treated with KCl or detergent. Type B seems to be a complex form of type A and some high-molecular-weight substance.

Full text

PDF
5

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fujimura S., Makino T., Hayashi T. The effect of bleomycin on prophage induction and staphylokinase production. Jpn J Exp Med. 1972 Feb;42(1):75–80. [PubMed] [Google Scholar]
  3. Fujimura S., Makino T., Hayashi T. [Effect of mitomycin C on the production of staphylokinase, nuclease and hemolysin by Staphylococcus aureus]. Nihon Saikingaku Zasshi. 1970 Jun;25(5):316–320. doi: 10.3412/jsb.25.316. [DOI] [PubMed] [Google Scholar]
  4. Fujumura S., Makino T., Hayashi T. Stimulation of staphylokinase production by mitomycin C. Naturwissenschaften. 1970 Aug;57(8):394–395. doi: 10.1007/BF00599983. [DOI] [PubMed] [Google Scholar]
  5. HAYASHI T., MAEKAWA S. Studies on the activating factors in fibrinolytic system. II. Streptococcal and staphylococcal fibrinolysis. Jpn J Exp Med. 1954 Oct;24(5):287–305. [PubMed] [Google Scholar]
  6. LEWIS J. H., FERGUSON J. H. A proteolytic enzyme system of the blood. III. Activation of dog serum profibrinolysin by staphylokinase. Am J Physiol. 1951 Sep;166(3):594–602. doi: 10.1152/ajplegacy.1951.166.3.594. [DOI] [PubMed] [Google Scholar]
  7. Liu T. H., Mertz E. T. Studies on plasminogen. IX. Purification of human plasminogen from Cohn fraction 3 by affinity chromatography. Can J Biochem. 1971 Sep;49(9):1055–1061. doi: 10.1139/o71-154. [DOI] [PubMed] [Google Scholar]
  8. Satoh Y. [On the purification of staphylokinase]. Sapporo Igaku Zasshi. 1969 Jan-Feb;35(1):67–77. [PubMed] [Google Scholar]
  9. Winkler K. C., de Waart J., Grootsen C. Lysogenic conversion of staphylococci to loss of beta-toxin. J Gen Microbiol. 1965 Jun;39(3):321–333. doi: 10.1099/00221287-39-3-321. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES