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. 1974 Jul;28(1):47–51. doi: 10.1128/am.28.1.47-51.1974

Use of Immunoperoxidase for the Rapid Identification of Human Myxoviruses and Paramyxoviruses in Tissue Culture

Denis R Benjamin 1, C George Ray 1
PMCID: PMC186585  PMID: 4367457

Abstract

The immunoperoxidase method, using commercially available antisera, was compared with standard virological methods for the identification and typing of 77 isolates of human myxoviruses and paramyxoviruses. Results of typing using neutralization tests and the immunoperoxidase technique were identical for 76 of the 77 isolates. With the immunoperoxidase method there was one false negative reaction, but no false positive reactions. Cross-reactivity between influenza A (soluble) and A2/HK antisera with influenza A isolates was noted, but did not interfere with the interpretation of results. It is concluded that the immunoperoxidase method is ideally suited for the rapid identification and typing of common human respiratory viruses on a routine basis. It offers a number of decided advantages over both immunofluorescence and standard virological methods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  2. Clyne D. H., Norris S. H., Modesto R. R., Pesce A. J., Pollak V. E. Antibody enzyme conjugates: the preparation of intermolecular conjugates of horseradish peroxidase and antibody and their use in immunohistology of renal cortex. J Histochem Cytochem. 1973 Mar;21(3):233–240. doi: 10.1177/21.3.233. [DOI] [PubMed] [Google Scholar]
  3. Cradock-Watson J. E., McQuillin J., Gardner P. S. Rapid diagnosis of respiratory syncytial virus infection in children by the immunofluorescent technique. J Clin Pathol. 1971 May;24(4):308–312. doi: 10.1136/jcp.24.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. D'Alessio D., Williams S., Dick E. C. Rapid detection and identification of respiratory viruses by direct immunofluorescence. Appl Microbiol. 1970 Aug;20(2):233–239. doi: 10.1128/am.20.2.233-239.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dobardzic R., Boudreault A., Pavilanis V. L'identification du virus de l'influenza à l'aide de l'immunoperoxydase. Can J Microbiol. 1973 Jan;19(1):146–149. [PubMed] [Google Scholar]
  6. Gardner P. S., McQuillin J. Application of immunofluorescent antibody technique in rapid diagnosis of respiratory syncytial virus infection. Br Med J. 1968 Aug 10;3(5614):340–343. doi: 10.1136/bmj.3.5614.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gray K. G., MacFarlane D. E., Sommerville R. G. Direct immunofluorescent identification of respiratory syncytial virus in throat swabs from children with respiratory illness. Lancet. 1968 Mar 2;1(7540):446–448. doi: 10.1016/s0140-6736(68)92779-7. [DOI] [PubMed] [Google Scholar]
  8. Herd S., Macwilliam K. Preparation of antiviral sera for immunofluorescence on infected tissue culture. J Clin Pathol. 1971 May;24(4):304–307. doi: 10.1136/jcp.24.4.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herrmann J. E., Morse S. A. Coupling of peroxidase to poliovirus antibody: characteristics of the conjugates and their use in virus detection. Infect Immun. 1973 Oct;8(4):645–649. doi: 10.1128/iai.8.4.645-649.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KAPLOW L. S. SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. Blood. 1965 Aug;26:215–219. [PubMed] [Google Scholar]
  11. Marks M. I., Nagahama H., Eller J. J. Parainfluenza virus immunofluorescence. In vitro and clinical application of the direct method. Pediatrics. 1971 Jul;48(1):73–78. [PubMed] [Google Scholar]
  12. Rahman A. F. Early detection of influenza virus in cell culture by means of immunofluorescence. J Clin Pathol. 1973 Jul;26(7):503–505. doi: 10.1136/jcp.26.7.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shabo A. L., Petricciani J. C., Kirschstein R. L. Immunoperoxidase localization of herpes zoster virus and simian virus 40 in cell culture. Appl Microbiol. 1972 May;23(5):1001–1009. doi: 10.1128/am.23.5.1001-1009.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stevens T. D., Watkins H. M. Rapid identification of viruses by indirect immunofluorescence: standardization and use of antiserum pool to nine respiratory viruses. Appl Microbiol. 1969 Mar;17(3):384–393. doi: 10.1128/am.17.3.384-393.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ubertini T., Wilkie B. N., Noronha F. Use of horseradish peroxidase-labeled antibody for light and electron microscope localization of reovirus antigen. Appl Microbiol. 1971 Mar;21(3):534–538. doi: 10.1128/am.21.3.534-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Urquhart G. E., Martin K. W. Respiratory syncytial virus tissue culture immunofluorescence as a laboratory aid. Arch Gesamte Virusforsch. 1970;32(4):365–372. doi: 10.1007/BF01250064. [DOI] [PubMed] [Google Scholar]
  17. Wicker R., Avrameas S. Localization of virus antigens by enzyme-labelled antibodies. J Gen Virol. 1969 Jun;4(4):465–471. doi: 10.1099/0022-1317-4-4-465. [DOI] [PubMed] [Google Scholar]
  18. Wicker R. Comparison of immunofluorescent and immunoenzymatic techniques applied to the study of viral antigens. Ann N Y Acad Sci. 1971 Jun 21;177:490–500. doi: 10.1111/j.1749-6632.1971.tb35079.x. [DOI] [PubMed] [Google Scholar]

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