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. 1974 Jul;28(1):86–90. doi: 10.1128/am.28.1.86-90.1974

Comparison of Purified Alpha-Toxins from Various Strains of Staphylococcus aureus

Ronald L Goode 1,1, Jack N Baldwin 1
PMCID: PMC186598  PMID: 4858502

Abstract

Alpha-toxin from five strains of Staphylococcus aureus, including Wood 46, was purified by isoelectric focusing. The alpha-toxins obtained from different strains were similar. The isoelectric point of the purified toxins was 8.65 ± 0.15. Sharp concentration peaks were not always obtained. In the ultracentrifuge the alpha-toxins migrated usually as three peaks which could be dissociated with propionic acid to yield one peak. A single line of identity was obtained in immunoelectrophoresis when a heterologous antiserum was reacted with the five purified toxins. It was concluded that the widespread use of the Wood 46 strain for the production of alpha-toxin is justified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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