Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1974 Jul;28(1):112–116. doi: 10.1128/am.28.1.112-116.1974

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Rodney F Smith 1,1, Dianna Blasi 1, Sandra L Dayton 1
PMCID: PMC186605  PMID: 4602310

Abstract

Six media were evaluated to determine their ability to isolate yeasts and inhibit bacteria. The media included the following: Snyder, Snyder tellurite, Sabouraud tellurite, Littman-gentamicin, molybdate, and Mycosel (BBL). Doses of mixed intestinal gram-negative bacilli and enterococci were most effectively inhibited by Snyder tellurite agar. Klebsiella pneumoniae was the most common bacterial contaminant of the other media. All six media were comparable in isolating yeasts while preventing the growth of the oral bacterial flora. The selection of a basal fungal growth medium for tellurite incorporation to inhibit bacteria but permit growth of yeasts was affected by pH. The bacteriostatic effect of tellurite was decreased with increasing pH of media while fungistatic action was increased. The arbitrary selection of Snyder and Littman agars to isolate yeast from burn wound cultures demonstrated the need to include a selective medium for these specimens. Blood, phenylethyl alcohol blood agar, and Columbia CN blood agar were all inadequate for isolating yeasts from burns. Growth of a variety of filamentous saprophytic and pathogenic dimorphic fungi grew adequately on four of five selective media tested.

Full text

PDF
112

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black W. A., Van Buskirk F. Gentamicin blood agar used as a general-purpose selective medium. Appl Microbiol. 1973 Jun;25(6):905–907. doi: 10.1128/am.25.6.905-907.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bump C. M., Kunz L. J. Routine identification of yeasts with the aid of molybdate-agar medium. Appl Microbiol. 1968 Oct;16(10):1503–1506. doi: 10.1128/am.16.10.1503-1506.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COSTA S. O., BRANCOCDE L. EVALUATION OF A MOLYBDENUM CULTURE MEDIUM AS SELECTIVE AND DIFFERENTIAL FOR YEASTS. J Pathol Bacteriol. 1964 Apr;87:428–431. doi: 10.1002/path.1700870226. [DOI] [PubMed] [Google Scholar]
  4. Dolan C. T. Evaluation of various media for growth of selected pathogenic fungi and Nocardia asteroides. Am J Clin Pathol. 1972 Sep;58(3):339–342. doi: 10.1093/ajcp/58.3.339. [DOI] [PubMed] [Google Scholar]
  5. HOLLAND M. L., KUNZ L. J. Evaluation of molybdate agar as a selective, differential medium for yeasts. J Bacteriol. 1961 Jun;81:869–874. doi: 10.1128/jb.81.6.869-874.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Law E. J., Kim O. J., Stieritz D. D., MacMillan B. G. Experience with systemic candidiasis in the burned patient. J Trauma. 1972 Jul;12(7):543–552. doi: 10.1097/00005373-197207000-00001. [DOI] [PubMed] [Google Scholar]
  7. Marks M. I., Langston C., Eickhoff T. C. Torulopsis glabrata--an opportunistic pathogen in man. N Engl J Med. 1970 Nov 19;283(21):1131–1135. doi: 10.1056/NEJM197011192832103. [DOI] [PubMed] [Google Scholar]
  8. Nash G., Foley F. D., Pruitt B. A., Jr Candida burn-wound invasion. A cause of systemic candidiasis. Arch Pathol. 1970 Jul;90(1):75–78. [PubMed] [Google Scholar]
  9. Salkin I. F. Dermatrophye test medium: evaluation with nondermatophytic pathogens. Appl Microbiol. 1973 Aug;26(2):134–137. doi: 10.1128/am.26.2.134-137.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith A. G., Rohde P. A. A new technique for isolating pathogenic fungi from mixed flora inocula. Mycopathol Mycol Appl. 1972 Jun 15;47(1):105–120. doi: 10.1007/BF02126158. [DOI] [PubMed] [Google Scholar]
  11. Smith R. F., Bettge C. L., Dayton S. L., Jorgensen J. H. Characterization of Staphylococcus aureus in a pediatric burn unit. Appl Microbiol. 1973 Jan;25(1):15–20. doi: 10.1128/am.25.1.15-20.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith R. F., Dayton S. L., Chipps D. D., Blasi D. Intestinal carriage of Klebsiella and pseudomonas in burned children and their comparative role in nosocomial infection. Health Lab Sci. 1973 Jul;10(3):173–179. [PubMed] [Google Scholar]
  13. Smith R. F., Dayton S. L. Use of acetamide broth in the isolation of Pseudomonas aeruginosa from rectal swabs. Appl Microbiol. 1972 Jul;24(1):143–145. doi: 10.1128/am.24.1.143-145.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taplin D., Zaias N., Rebell G., Blank H. Isolation and recognition of dermatophytes on a new medium (DTM). Arch Dermatol. 1969 Feb;99(2):203–209. [PubMed] [Google Scholar]
  15. Vogel R. A. Primary isolation medium for Cryptococcus neoformans. Appl Microbiol. 1969 Dec;18(6):1100–1100. doi: 10.1128/am.18.6.1100-1100.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES