. Author manuscript; available in PMC: 2007 May 10.

Published in final edited form as: Biochemistry. 2001 Sep 4;40(35):10614–10624. doi: 10.1021/bi010672h

Table 2.

Steady-State Parameters of rGSTA1-1 and Site-Directed Mutants

enzyme	k_cat (s⁻¹)	K_M (μM)	k_cat/K_M (mM⁻¹ s⁻¹)
CDNB
WT^a	52 ± 2	560 ± 60	93 ± 6
W21F^a	47 ± 5	520 ± 50	90 ± 9
F10Y:W21F	43 ± 2	753 ± 119	57 ± 2
F10A:W21F	12 ± 1	585 ± 119	20 ± 4
F10L:W21F	25 ± 2	479 ± 66	51 ± 1
W21F:F220Y	55 ± 7	410 ± 40	134 ± 14
W21F:F220I	14 ± 2	300 ± 14	47 ± 6
GSH
WT^a	51 ± 2	310 ± 30	164 ± 12
W21F^a	62 ± 6	370 ± 30	167 ± 12
F10Y:W21F	43 ± 1	1014 ± 133	42 ± 1
F10A:W21F	12 ± 1	714 ± 94	17 ± 3
F10L:W21F	26 ± 1	551 ± 98	47 ± 3
W21F:F220Y	65 ± 7	331 ± 19	196 ± 19
W21F:F220I	14 ± 2	330 ± 31	42 ± 7

Values taken from ref 44.