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. 1974 Aug;28(2):258–261. doi: 10.1128/am.28.2.258-261.1974

Rapid Method for the Radioisotopic Analysis of Gaseous End Products of Anaerobic Metabolism

David R Nelson 1, J G Zeikus 1
PMCID: PMC186697  PMID: 4854029

Abstract

A gas chromatographic procedure for the simultaneous analysis of 14C-labeled and unlabeled metabolic gases from microbial methanogenic systems is described. H2, CH4, and CO2 were separated within 2.5 min on a Carbosieve B column and were detected by thermal conductivity. Detector effluents were channeled into a gas proportional counter for measurement of radioactivity. This method was more rapid, sensitive, and convenient than gas chromatography-liquid scintillation techniques. The gas chromatography-gas proportional counting procedure was used to characterize the microbial decomposition of organic matter in anaerobic lake sediments and to monitor 14CH4 formation from H2 and 14CO2 by Methanosarcina barkeri.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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