Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1974 Aug;28(2):298–302. doi: 10.1128/am.28.2.298-302.1974

Rapid Diagnosis of Infection by Gas-Liquid Chromatography: Analysis of Sugars in Normal and Infected Cerebrospinal Fluid

Stanley Amundson 1, Abraham I Braude 1, Charles E Davis 1
PMCID: PMC186704  PMID: 4854025

Abstract

A highly reproducible procedure was developed for gas-liquid chromatographic analysis of trimethylsilyl derivatives of normal human cerebrospinal fluid. Fourteen normal human cerebrospinal fluid samples tested with this procedure contained α- and β-glucose as well as isomers of two unidentified sugars. Chromatographic changes in three cases of meningeal inflammation (two cryptococcosis and one thalamic astrocytoma) were limited to decreased concentrations of all sugars. In one case of early meningitis, the concentrations of the unknown sugars decreased before glucose. Now that a reproducible chromatogram of the trimethylsilyl derivatives of normal human cerebrospinal fluid has been established, more samples of abnormal cerebrospinal fluid should be prepared by these methods and examined by gas-liquid chromatography. It may be possible to identify unique products of infectious agents which will permit rapid diagnosis of central nervous system infection.

Full text

PDF
298

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnett D., Cohen R. D., Tassopoulos C. N., Turtle J. R., Dimitriadou A., Fraser T. R. A method for estimation of Krebs cycle and related intermediates in animal tissues by gas chromatography. Anal Biochem. 1968 Oct 10;26(1):68–84. doi: 10.1016/0003-2697(68)90032-8. [DOI] [PubMed] [Google Scholar]
  2. Berry J. F., Bovis M., Logothetis J. Determination of the fatty acid composition of cerebrospinal fluid by gas-liquid chromatography. Neurology. 1965 Dec;15(12):1089–1094. doi: 10.1212/wnl.15.12.1089. [DOI] [PubMed] [Google Scholar]
  3. Constantopoulos G., Dekaban A. S. Acid mucopolysaccharides in the cerebrospinal fluid of patients with Hunter-Hurler's syndrome. J Neurochem. 1970 Jan;17(1):117–120. doi: 10.1111/j.1471-4159.1970.tb00508.x. [DOI] [PubMed] [Google Scholar]
  4. Davis C. E., Freedman S. D., Douglas H., Braude A. I. Analysis of sugars in bacterial endotoxins by gas-liquid chromatography. Anal Biochem. 1969 Apr 4;28(1):243–256. doi: 10.1016/0003-2697(69)90175-4. [DOI] [PubMed] [Google Scholar]
  5. Doelle H. W., Manderson G. J. Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products. Antonie Van Leeuwenhoek. 1969;35(4):467–478. doi: 10.1007/BF02219165. [DOI] [PubMed] [Google Scholar]
  6. Gottfries C. G., Gottfries I., Roos B. E. Homovanillic acid and 5-hydroxyindoleacetic acid in the cerebrospinal fluid of patients with senile dementia, presenile dementia and parkinsonism. J Neurochem. 1969 Sep;16(9):1341–1345. doi: 10.1111/j.1471-4159.1969.tb05984.x. [DOI] [PubMed] [Google Scholar]
  7. Hammond K. B., Goodman S. I. A gas chromatographic procedure for detection of pathological organic aciduria. Clin Chem. 1970 Mar;16(3):212–214. [PubMed] [Google Scholar]
  8. Mitruka B. M., Jonas A. M., Alexander M. Rapid detection of bacteremia in mice by gas chromatography. Infect Immun. 1970 Oct;2(4):474–478. doi: 10.1128/iai.2.4.474-478.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Waterbury L. D., Pearce L. A. Separation and identification of neutral and acidic metabolites in cerebrospinal fluid. Clin Chem. 1972 Mar;18(3):258–262. [PubMed] [Google Scholar]
  10. Wilk S., Davis K. L., Thacker S. B. Determination of 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) in cerebrospinal fluid. Anal Biochem. 1971 Feb;39(2):498–504. doi: 10.1016/0003-2697(71)90440-4. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES