Abstract
Freshly prepared 2% acid and alkaline glutaraldehyde solutions were stored at 4, 20, and 37 C. At intervals, samples were removed and changes in pH, ultraviolet spectrum, and sporicidal activity (against Bacillus pumilus spores) were recorded. Alkaline solutions stored at 4 C showed little changes in these properties, whereas such solutions stored at 37 C became turbid and showed a decrease in pH, marked changes in ultraviolet spectrum, and an almost complete loss of sporicidal activity. Intermediate results were obtained with alkaline solutions stored at 20 C. In contrast, acid 2% glutaraldehyde solutions (initial pH 3.5) showed comparatively few changes in their properties. Treatment of spores with freshly prepared glutaraldehyde solutions (0.5%) at temperature above 40 C reduced the effect of pH on sporicidal activity.
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- Borick P. M. Chemical sterilizers (chemosterilizers). Adv Appl Microbiol. 1968;10:291–312. doi: 10.1016/s0065-2164(08)70195-3. [DOI] [PubMed] [Google Scholar]
- Hopwood D., Callen C. R., McCabe M. The reactions between glutaraldehyde and various proteins. An investigation of their kinetics. Histochem J. 1970 Mar;2(2):137–150. doi: 10.1007/BF01003541. [DOI] [PubMed] [Google Scholar]
- Korn A. H., Feairheller S. H., Filachione E. M. Glutaraldehyde: nature of the reagent. J Mol Biol. 1972 Apr 14;65(3):525–529. doi: 10.1016/0022-2836(72)90206-9. [DOI] [PubMed] [Google Scholar]
- Kuipers J. S., Tromp F. J. Bactericide eigenschappen van glutaaraldehyde. Pharm Weekbl. 1973 Mar 2;108(9):169–173. [PubMed] [Google Scholar]
- Munton T. J., Russel A. D. Interaction of glutaraldehyde with spheroplasts of Escherichia coli. J Appl Bacteriol. 1973 Jun;36(2):211–217. doi: 10.1111/j.1365-2672.1973.tb04093.x. [DOI] [PubMed] [Google Scholar]
- Munton T. J., Russell A. D. Aspects of the action of glutaraldehyde on Escherichia coli. J Appl Bacteriol. 1970 Jun;33(2):410–419. doi: 10.1111/j.1365-2672.1970.tb02214.x. [DOI] [PubMed] [Google Scholar]
- Munton T. J., Russell A. D. Effect of glutaraldehyde on the outer layers of Escherichia coli. J Appl Bacteriol. 1972 Jun;35(2):193–199. doi: 10.1111/j.1365-2672.1972.tb03690.x. [DOI] [PubMed] [Google Scholar]
- Rubbo S. D., Gardner J. F., Webb R. L. Biocidal activities of glutaraldehyde and related compounds. J Appl Bacteriol. 1967 Apr;30(1):78–87. doi: 10.1111/j.1365-2672.1967.tb00277.x. [DOI] [PubMed] [Google Scholar]
- Sierra G., Boucher R. M. Ultrasonic synergistic effects in liquid-phase chemical sterilization. Appl Microbiol. 1971 Aug;22(2):160–164. doi: 10.1128/am.22.2.160-164.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]