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. 1974 Sep;28(3):336–339. doi: 10.1128/am.28.3.336-339.1974

Formations of Extracellular Isoamylase and Intracellular α-Glucosidase and Amylase(s) by Pseudomonas SB15 and a Mutant Strain

Toshiyuki Sugimoto 1, Akinori Amemura 1, Tokuya Harada 1
PMCID: PMC186720  PMID: 4418042

Abstract

Pseudomonas SB15, which produces extracellular isoamylase, was found to produce intracellular α-glucosidase and amylase(s) when grown on maltose. A mutant strain (MS1) derived from it, which formed isoamylase constitutively, also produced these intracellular enzymes constitutively. The activities of the enzymes produced in the mutant strain were much greater than those induced in the parent strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akai H., Yokobayashi K., Misaki A., Harada T. Complete hydrolysis of branching linkages in glycogen by Pseudomonas isoamylase: distribution of linear chains. Biochim Biophys Acta. 1971 Jun 22;237(3):422–429. doi: 10.1016/0304-4165(71)90259-5. [DOI] [PubMed] [Google Scholar]
  2. Akai H., Yokobayashi K., Misaki A., Harada T. Structural analysis of amylopectin using Pseudomonas isoamylase. Biochim Biophys Acta. 1971 Dec 21;252(3):427–431. doi: 10.1016/0304-4165(71)90144-9. [DOI] [PubMed] [Google Scholar]
  3. Harada T., Misaki A., Akai H., Yokobayashi K., Sugimoto K. Characterization of Pseudomonas isoamylase by its actions on amylopectin and glycogen: comparison with Aerobacter pullulanase. Biochim Biophys Acta. 1972 May 12;268(2):497–505. doi: 10.1016/0005-2744(72)90345-2. [DOI] [PubMed] [Google Scholar]
  4. Harada T., Yokobayashi K., Misaki A. Formation of isoamylase by Pseudomonas. Appl Microbiol. 1968 Oct;16(10):1439–1444. doi: 10.1128/am.16.10.1439-1444.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Yokobayashi K., Akai H., Sugimoto T., Hirao M., Sugimoto K., Harada T. Comparison of the kinetic parameters of Pseudomonas isoamylase and Aerobacter pullulanase. Biochim Biophys Acta. 1973 Jan 12;293(1):197–202. doi: 10.1016/0005-2744(73)90391-4. [DOI] [PubMed] [Google Scholar]
  7. Yokobayashi K., Misaki A., Harada T. Purification and properties of Pseudomonas isoamylase. Biochim Biophys Acta. 1970 Sep 16;212(3):458–469. doi: 10.1016/0005-2744(70)90252-4. [DOI] [PubMed] [Google Scholar]

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