Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1974 Sep;28(3):351–354. doi: 10.1128/am.28.3.351-354.1974

Stability of Minute Virus of Mice to Chemical and Physical Agents

Robert E Harris 1,1, Philip H Coleman 1, Page S Morahan 1
PMCID: PMC186724  PMID: 4213983

Abstract

Minute virus of mice (MVM), a single-stranded deoxyribonucleic acid Parvovirus, was subjected to various inactivation procedures, including chemical disinfectants, heat, and ultraviolet radiation. MVM was found to be less stable than has been reported for other Parvoviruses. This virus was readily inactivated by a variety of chemical disinfectants, including alcohols, formaldehyde, glutaraldehyde, and chloroform. MVM was more sensitive to ultraviolet radiation than was Kilham's rat virus. MVM was more sensitive to heating at temperatures of 35 to 100 C than has been reported for other Parvoviruses. More than 95% of MVM infectivity was inactivated by heating (45, 60, or 100 C) for 60 min, acid (pH 2.0) treatment, or ultraviolet radiation treatment, although a small percentage (less than 2%) of the virus preparation was found to be resistant to these treatments. In addition, more than 99% of the infectivity of MVM was lost after storage at 4C for 10 weeks, although the virus was stable on storage in liquid nitrogen.

Full text

PDF
351

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann P. A. Porcine parvovirus infection in vitro: a study model for the replication of parvoviruses. I. Replication at different temperatures. 1. Proc Soc Exp Biol Med. 1972 Sep;140(4):1369–1374. doi: 10.3181/00379727-140-36676. [DOI] [PubMed] [Google Scholar]
  2. Collins M. J., Jr, Parker J. C. Murine virus contaminants of leukemia viruses and transplantable tumors. J Natl Cancer Inst. 1972 Oct;49(4):1139–1143. [PubMed] [Google Scholar]
  3. Crawford L. V. A minute virus of mice. Virology. 1966 Aug;29(4):605–612. doi: 10.1016/0042-6822(66)90284-4. [DOI] [PubMed] [Google Scholar]
  4. GREENE E. L. PHYSICAL AND CHEMICAL PROPERTIES OF H-1 VIRUS. I. PH AND HEAT STABILITY OF THE HEMAGGLUTINATING PROPERTY. Proc Soc Exp Biol Med. 1965 Apr;118:973–975. doi: 10.3181/00379727-118-30020. [DOI] [PubMed] [Google Scholar]
  5. KILHAM L., OLIVIER L. J. A latent virus of rats isolated in tissue culture. Virology. 1959 Apr;7(4):428–437. doi: 10.1016/0042-6822(59)90071-6. [DOI] [PubMed] [Google Scholar]
  6. Kilham L., Margolis G. Pathogenicity of minute virus of mice (MVM) for rats, mice, and hamsters. Proc Soc Exp Biol Med. 1970 Apr;133(4):1447–1452. doi: 10.3181/00379727-133-34710. [DOI] [PubMed] [Google Scholar]
  7. Parker J. C., Collins M. J., Jr, Cross S. S., Rowe W. P. Minute virus of mice. II. Prevalence, epidemiology, and occurrence as a contaminant of transplanted tumors. J Natl Cancer Inst. 1970 Aug;45(2):305–310. [PubMed] [Google Scholar]
  8. Parker J. C., Cross S. S., Collins M. J., Jr, Rowe W. P. Minute virus of mice. I. Procedures for quantitation and detection. J Natl Cancer Inst. 1970 Aug;45(2):297–303. [PubMed] [Google Scholar]
  9. Poole G. M. Stability of a modified, live panleucopenia virus stored in liquid phase. Appl Microbiol. 1972 Oct;24(4):663–664. doi: 10.1128/am.24.4.663-664.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Proctor W. R., Cook J. S., Tennant R. W. Ultraviolet photobiology of Kilham rat virus and the absolute ultraviolet photosensitivities of other animal viruses: influence of DNA strandedness, molecular weight, and host-cell repair. Virology. 1972 Aug;49(2):368–378. doi: 10.1016/0042-6822(72)90489-8. [DOI] [PubMed] [Google Scholar]
  11. ROWE W. P., HARTLEY J. W., ESTES J. D., HUEBNER R. J. Growth curves of polyoma virus in mice and hamsters. Natl Cancer Inst Monogr. 1960 Sep;4:189–209. [PubMed] [Google Scholar]
  12. Schell K., Lane W. T., Casey M. J., Huebner R. J. Potentiation of oncogenicity of adenovirus type 12 grown in African green monkey kidney cell cultures preinfected with SV40 virus. Persistence of both T antigens in the tumors and evidence for possible hybridization. Proc Natl Acad Sci U S A. 1966 Jan;55(1):81–88. doi: 10.1073/pnas.55.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scholander P. F. The role of solvent pressure in osmotic systems. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1407–1414. doi: 10.1073/pnas.55.6.1407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. Purification of rabies virus grown in tissue culture. J Virol. 1968 Aug;2(8):836–849. doi: 10.1128/jvi.2.8.836-849.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Toolan H. W. The picodna viruses. H, RV, and AAV. Int Rev Exp Pathol. 1968;6:135–180. [PubMed] [Google Scholar]
  16. WALLIS C., MELNICK J. L. CYSTINE DEPENDENCE OF THERMORESISTANT MUTANTS OF POLIOVIRUS. J Bacteriol. 1965 Apr;89:1160–1161. doi: 10.1128/jb.89.4.1160-1161.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wallis C., Melnick J. L. Virus aggregation as the cause of the non-neutralizable persistent fraction. J Virol. 1967 Jun;1(3):478–488. doi: 10.1128/jvi.1.3.478-488.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES