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. 1974 Sep;28(3):435–438. doi: 10.1128/am.28.3.435-438.1974

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

M Caslow 1, P D Ellner 1, T E Kiehn 1
PMCID: PMC186738  PMID: 4607595

Abstract

One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed on aerobic and hypertonic vials at 24 h and 7 days, and on anaerobic vials at 48 h and 7 days. Significant numbers of false-positive BACTEC readings were obtained. Although all positive cultures were eventually detected by the BACTEC, approximately 20% of blood specimens yielding positive subcultures at 24 h did not give positive BACTEC readings until 48 h.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DeBlanc H. J., Jr, DeLand F., Wagner H. N., Jr Automated radiometric detection of bacteria in 2,967 blood cultures. Appl Microbiol. 1971 Nov;22(5):846–849. doi: 10.1128/am.22.5.846-849.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DeLand F. H., Wagner H. N., Jr Early detection of bacterial growth, with carbon-14-labeled glucose. Radiology. 1969 Jan;92(1):154–155. doi: 10.1148/92.1.154. [DOI] [PubMed] [Google Scholar]
  3. DeLand F., Wagner H. N., Jr Automated radiometric detection of bacterial growth in blood cultures. J Lab Clin Med. 1970 Mar;75(3):529–534. [PubMed] [Google Scholar]
  4. Ellner P. D., Granato P. A., May C. B. Recovery and identification of anaerobes: a system suitable for the routine clinical laboratory. Appl Microbiol. 1973 Dec;26(6):904–913. doi: 10.1128/am.26.6.904-913.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellner P. D. System for inoculation of blood in the laboratory. Appl Microbiol. 1968 Dec;16(12):1892–1894. doi: 10.1128/am.16.12.1892-1894.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Renner E. D., Gatheridge L. A., Washington J. A., 2nd Evaluation of radiometric system for detecting bacteremia. Appl Microbiol. 1973 Sep;26(3):368–372. doi: 10.1128/am.26.3.368-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Washington J. A., 2nd, Yu P. K. Radiometric method for detection of bacteremia. Appl Microbiol. 1971 Jul;22(1):100–101. doi: 10.1128/am.22.1.100-101.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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