Abstract
Rickettsia rickettsii was treated with ether and examined by negative-contrast electron microscopy. Group-specific complement-fixing antigen was seen to be originating from the cell wall. The antigen was composed predominately of round particles 10 to 60 nm in diameter. Intact R. rickettsii and antigen from ether-treated organisms were purified by density gradient centrifugation and analyzed by polyacrylamide gel electrophoresis. The whole rickettsial cell was composed of a minimum of 30 proteins which ranged in molecular weight from about 23,000 to 155,000. The “soluble” antigen contained nine proteins ranging in molecular weight from about 28,000 to 150,000.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anacker R. L., Pickens E. G., Lackman D. B. Details of the ultrastructure of Rickettsia prowazekii grown in the chick yolk sac. J Bacteriol. 1967 Jul;94(1):260–262. doi: 10.1128/jb.94.1.260-262.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Balayeva N. M., Zubok L. P., Nikolskaya V. N. Soluble antigen of Rickettsia prowazeki. Acta Virol. 1966 Mar;10(2):161–168. [PubMed] [Google Scholar]
- Barker L. S., Patt J. K. Antigens of Rickettsia prowazeki. I. Activity in complement fixation and mouse protection tests. J Immunol. 1968 Mar;100(3):543–547. [PubMed] [Google Scholar]
- Brezina R. Advances in rickettsial research. Curr Top Microbiol Immunol. 1969;47:20–39. doi: 10.1007/978-3-642-46160-6_2. [DOI] [PubMed] [Google Scholar]
- FRYGIN C. [Electrophoretic, immunoelectrophoretic and chromatographic analysis of the soluble antigen of Rickettsia prowazekli]. Med Dosw Mikrobiol. 1963;15:29–42. [PubMed] [Google Scholar]
- GIMENEZ D. F. STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964 May;39:135–140. doi: 10.3109/10520296409061219. [DOI] [PubMed] [Google Scholar]
- Obijeski J. F., Marchenko A. T., Bishop D. H., Cann B. W., Murphy F. A. Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. J Gen Virol. 1974 Jan;22(1):21–33. doi: 10.1099/0022-1317-22-1-21. [DOI] [PubMed] [Google Scholar]
- PERKINS H. R., ALLISON A. C. Cell-wall constituents of rickettsiae and psittacosis-lymphogranuloma organisms. J Gen Microbiol. 1963 Mar;30:469–480. doi: 10.1099/00221287-30-3-469. [DOI] [PubMed] [Google Scholar]
- Palmer E. L., Mallavia L. P., Tzianabos T., Obijeski J. F. Electron microscopy of the cell wall of Rickettsia prowazeki. J Bacteriol. 1974 Jun;118(3):1158–1166. doi: 10.1128/jb.118.3.1158-1166.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHAECHTER M., TOUSIMIS A. J., COHN Z. A., ROSEN H., CAMPBELL J., HAHN F. E. Morphological, chemical, and serological studies of the cell walls of Rickettsia mooseri. J Bacteriol. 1957 Dec;74(6):822–829. doi: 10.1128/jb.74.6.822-829.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOENNER H. G., LACKMAN D. B., BELL E. J. Factors affecting the growth of rickettsias of the spotted fever group in fertile hens' eggs. J Infect Dis. 1962 Mar-Apr;110:121–128. doi: 10.1093/infdis/110.2.121. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
- Wood W. H., Jr, Wisseman C. L., Jr The cell wall of Rickettsia mooseri. I. Morphology and chemical composition. J Bacteriol. 1967 Mar;93(3):1113–1118. doi: 10.1128/jb.93.3.1113-1118.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]