Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1974 Sep;28(3):503–504. doi: 10.1128/am.28.3.503-504.1974

Improved Rapid Plate Method for the Isolation of Bacteriophages from Lysogenic Bacteria

Joseph T Parisi 1, Henry W Talbot Jr 1
PMCID: PMC186751  PMID: 4278902

Abstract

A modification of a recently reported rapid plate method for the isolation of bacteriophages from lysogenic bacteria is described. The velveteen replica plate technique was used for inoculation of mitomycin C-induced colonies onto agar plates, and tetrazolium chloride was used to enhance detection of phage activity on replicated indicator plates.

Full text

PDF
503

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Pattee P. A. Use of tetrazolium for improved resolution of bacteriophage plaques. J Bacteriol. 1966 Sep;92(3):787–788. doi: 10.1128/jb.92.3.787-788.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  4. Siddiqui A., Hart E., Mandagere U., Goldberg I. D. Rapid plate method for the isolation of lysogenic bacteriophages. Appl Microbiol. 1974 Jan;27(1):278–280. doi: 10.1128/am.27.1.278-280.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES