Abstract
A microneutralization test is described, in which secondary pig thyroid cells are used and end points are determined by hemadsorption with hamster erythrocytes. The method is evaluated and compared with a macromethod, in which the fluorescent-antibody staining technique is applied for reading titers. The micromethod was found to be useful because of its sensitivity and rapidity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cartwright S. F., Lucas M. Vomiting and wasting disease in piglets. Virological and epidemiological studies. Vet Rec. 1970 Mar 7;86(10):278–280. doi: 10.1136/vr.86.10.278. [DOI] [PubMed] [Google Scholar]
- Greig A. S., Girard A. Serological comparison of hemagglutinating encephalomyelitis viruses isolated from different outbreaks. Can J Comp Med. 1969 Jan;33(1):25–28. [PMC free article] [PubMed] [Google Scholar]
- Mengeling W. L. Porcine coronaviruses: co-infection of cell cultures with transmissible gastroenteritis virus and hemagglutinating encephalomyelitis virus. Am J Vet Res. 1973 Jun;34(6):779–783. [PubMed] [Google Scholar]
- Pirtle E. C. Titration of two porcine respiratory viruses in mammalian cell cultures by direct fluorescent antibody staining. Am J Vet Res. 1974 Feb;35(2):249–250. [PubMed] [Google Scholar]
- Ressang A. A. [Application of the immunofluorescence technic in the diagnosis of classical swine pest]. Bull Off Int Epizoot. 1970 Nov-Dec;73(11):999–1023. [PubMed] [Google Scholar]
- The T. H., Feltkamp T. E. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology. 1970 Jun;18(6):875–881. [PMC free article] [PubMed] [Google Scholar]