Abstract
Sera from a survey of 6,026 people were tested for hepatitis B surface antigen by using radioimmunoassay and counterelectrophoresis. Forty-eight sera (0.79%) were positive by counterelectrophoresis and 152 sera (2.52%) were positive by radioimmunoassay, using the most liberal of the recommended criteria for positivity (i.e., counts 3 standard deviations above the mean). Absorption tests performed on the 152 radioimmunoassay-positive sera showed that 10 (6.6%) were false-positive reactions to guinea pig protein, 74 (48.6%) were due to false-positive reaction(s) with other protein(s) in the test system, and 68 (44.8%) were true positives. There was a strong correlation between the degree of elevation of radioactive counts and the proportions of sera that were true positives; all 49 sera with counts >50 standard deviation units above the mean were true positives, but only 19 (18.4%) of the 103 sera with counts <50 standard deviation units were true positives. A few sera with high counts required absorption with type-specific (type D) antisera. The following conclusions were reached from this study: (i) absorption tests should be run on all radioimmunoassay-positive, counterelectrophoresis-negative sera; (ii) most (about 90%) false positives are not due to anti-guinea-pig protein reactions; and (iii) radioimmunoassay, in combination with absorption tests, yields a modest increase (about 35%) in detection of true positives over use of counterelectrophoresis alone.
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- Alter H. J., Holland P. V., Purcell R. H., Gerin J. L. The Ausria test: critical evaluation of sensitivity and specificity. Blood. 1973 Dec;42(6):947–957. [PubMed] [Google Scholar]
- Alter H. J., Polesky H. F., Holland P. V. False positive tests for hepatitis-associated antigen in blood donors caused by antibodies to ruminant serum proteins. J Immunol. 1972 Feb;108(2):358–369. [PubMed] [Google Scholar]
- Bancroft W. H., Mundon F. K., Russell P. K. Detection of additional antigenic determinants of hepatitis B antigen. J Immunol. 1972 Oct;109(4):842–848. [PubMed] [Google Scholar]
- Cossart Y. E., Boxall E., Flewett T. H. False-negative radioimmunoassay tests for Australia antigen. Lancet. 1973 Dec 8;2(7841):1333–1334. doi: 10.1016/s0140-6736(73)92916-4. [DOI] [PubMed] [Google Scholar]
- Ginsberg A. L., Conrad M. E., Bancroft W. H., Ling C. M., Overby L. R. Prevention of endemic HAA-positive hepatitis with gamma globulin. Use of a simple radioimmune assay to detect HAA. N Engl J Med. 1972 Mar 16;286(11):562–566. doi: 10.1056/NEJM197203162861102. [DOI] [PubMed] [Google Scholar]
- Leers W. D., Kouroupis G. M. Letter: R.I.A. kit for assay of hepatitis-B antigen. Lancet. 1973 Dec 8;2(7841):1334–1335. doi: 10.1016/s0140-6736(73)92917-6. [DOI] [PubMed] [Google Scholar]
- Ling C. M., Overby L. R. Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody. J Immunol. 1972 Oct;109(4):834–841. [PubMed] [Google Scholar]
- Prince A. M., Brotman B., Jass D., Ikram H. Specificity of the direct solid-phase radioimmunoassay for detection of hepatitis-B antigen. Lancet. 1973 Jun 16;1(7816):1346–1350. doi: 10.1016/s0140-6736(73)91674-7. [DOI] [PubMed] [Google Scholar]
- Sgouris J. T. Limitations of the radioimmunoassay for hepatitis B antigen (HB Ag). N Engl J Med. 1973 Jan 18;288(3):160–161. doi: 10.1056/NEJM197301182880317. [DOI] [PubMed] [Google Scholar]
- Vyas G. N., Adelberg S. G., Perkins H. A. Letter: Nonspecificity of hepatitis B antigen detected with iodine-125-labeled antibody. Science. 1973 Dec 28;182(4119):1368–1371. doi: 10.1126/science.182.4119.1368. [DOI] [PubMed] [Google Scholar]