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. 1974 Oct;28(4):608–613. doi: 10.1128/am.28.4.608-613.1974

Acetylene Reduction (Nitrogen Fixation) by Pulp and Paper Mill Effluents and by Klebsiella Isolated from Effluents and Environmental Situations

R Knowles 1, R Neufeld 1,1, S Simpson 1
PMCID: PMC186782  PMID: 4425455

Abstract

High rates of acetylene (C2H2) reduction (nitrogenase activity) were observed in woodroom effluent from a neutral sulfite semi-chemical mill under aerobic (up to 644 nmol of C2H4 produced per ml per h) and under anaerobic (up to 135 nmol of C2H4 produced per ml per h) conditions. Pasteurized effluent developed C2H2 reduction activity when incubated under anaerobic but not under aerobic conditions. Activities were increased by addition of 0.5 to 3.0% glucose or xylose. Enrichment and enumeration studies showed that N2-fixing Azotobacter and Klebsiella were abundant, and N2-fixing Bacillus was present. Of 129 isolates of Klebsiella from pulp mills, lakes, rivers, and drainage and sewage systems, 32% possessed nitrogen-fixing ability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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