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. 1974 Nov;28(5):836–839. doi: 10.1128/am.28.5.836-839.1974

Simplified Extraction Procedure for Serological Grouping of Beta-Hemolytic Streptococci

Aziz El Kholy 1, Lewis W Wannamaker 1,1, Richard M Krause 1,2
PMCID: PMC186834  PMID: 4216292

Abstract

An adaptation of the nitrous acid extraction of streptococci proved to be a reliable and practical method for the preparation of extracts for routine serological group identification. The extracts of all groups tested gave strong capillary precipitin reactions as well as reactions of double diffusion in gel. For routine grouping, extracts were prepared from the first one-half-plate subculture of the initial throat culture. The technique is simple and reliable, and it requires a minimum of technical skill, reagents, and equipment. Its use would facilitate epidemiological surveillance of group A streptococci and rapid diagnosis of streptococcal infections at a low cost.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ELLIOTT S. D. Type and group polysaccharides of group D streptococci. J Exp Med. 1960 May 1;111:621–630. doi: 10.1084/jem.111.5.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. MAXTED W. R. The use of bacitracin for identifying group A haemolytic streptococci. J Clin Pathol. 1953 Aug;6(3):224–226. doi: 10.1136/jcp.6.3.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Maxted W. R., Widdowson J. P., Fraser C. A., Ball L. C., Bassett D. C. The use of the serum opacity reaction in the typing of group-A streptococci. J Med Microbiol. 1973 Feb;6(1):83–90. doi: 10.1099/00222615-6-1-83. [DOI] [PubMed] [Google Scholar]
  4. McCARTY M. Further studies on the chemical basis for serological specificity of Group A streptococcal carbohydrate. J Exp Med. 1958 Sep 1;108(3):311–323. doi: 10.1084/jem.108.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Moody M. D., Siegel A. C., Pittman B., Winter C. C. Fluorescent-Antibody Identification of Group A Streptococci from Throat Swabs. Am J Public Health Nations Health. 1963 Jul;53(7):1083–1092. doi: 10.2105/ajph.53.7.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moody M. D., Webb C. D., Jr Identification of group A streptococci by direct fluorometry. Appl Microbiol. 1969 Apr;17(4):627–633. doi: 10.1128/am.17.4.627-633.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rotta J., Krause R. M., Lancefield R. C., Everly W., Lackland H. New approaches for the laboratory recognition of M types of group A streptococci. J Exp Med. 1971 Nov 1;134(5):1298–1315. doi: 10.1084/jem.134.5.1298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. el-Kholy A., Sorour A. H., Houser H. B., Wannamaker L. W., Robins M., Poitras J. M., Krause R. M. A three-year prospective study of streptococcal infections in a population of rural Egyptian school children. J Med Microbiol. 1973 Feb;6(1):101–110. doi: 10.1099/00222615-6-1-101. [DOI] [PubMed] [Google Scholar]

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