Abstract
Clostridium botulinum type F progenitor toxin was purified to a homogeneous state as judged by gel filtration on Sephadex G-200, ultracentrifugation, and disc electrophoresis. The sedimentation constant, corrected to water at 20 C, of type F progenitor toxin was determined to be 10.3 and the molecular weight to be 235,000 by ultracentrifugation at pH 6.0. The purified toxin contained a toxicity of 1.2 × 108 50% lethal doses/mg of N. In agar gel double diffusion, it formed two precipitin lines at pH 6.0. The progenitor toxin of type F differs from that of type A in that it contains no hemagglutinin and from that of type E in that it is not activable.
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Selected References
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- Beers W. H., Reich E. Isolation and characterization of Clostridium botulinum type B toxin. J Biol Chem. 1969 Aug 25;244(16):4473–4479. [PubMed] [Google Scholar]
- Boroff D. A., Fleck U. Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum. J Bacteriol. 1966 Nov;92(5):1580–1581. doi: 10.1128/jb.92.5.1580-1581.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARDELLA M. A., DUFF J. T., GOTTFRIED C., BEGEL J. S. Studies on immunity to toxins of Clostridium botulinum. IV. Production and purification of type C toxin for conversion to toxoid. J Bacteriol. 1958 Mar;75(3):360–365. doi: 10.1128/jb.75.3.360-365.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARDELLA M. A., DUFF J. T., WINGFIELD B. H., GOTTFRIED C. Studies on immunity to toxins of Clostridium botulinum. VI. Purification and detoxification of type D toxin and the immunological response to toxoid. J Bacteriol. 1960 Mar;79:372–378. doi: 10.1128/jb.79.3.372-378.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUFF J. T., KLERER J., BIBLER R. H., MOORE D. E., GOTTFRIED C., WRIGHT G. G. Studies on immunity to toxins of Clostridium botulinum. II. Production and purification of type B toxin for toxoid. J Bacteriol. 1957 May;73(5):597–601. doi: 10.1128/jb.73.5.597-601.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUFF J. T., WRIGHT G. G., KLERER J., MOORE D. E., BIBLER R. H. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. J Bacteriol. 1957 Jan;73(1):42–47. doi: 10.1128/jb.73.1.42-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DasGupta B. R. Activation of Clostridium botulinum type B toxin by an endogenous enzyme. J Bacteriol. 1971 Dec;108(3):1051–1057. doi: 10.1128/jb.108.3.1051-1057.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DasGupta B. R., Boroff D. A., Cheong K. Isolation of chromatographically pure toxin of Clostridium botulinum type B. Biochem Biophys Res Commun. 1968 Sep 30;32(6):1057–1063. doi: 10.1016/0006-291x(68)90137-x. [DOI] [PubMed] [Google Scholar]
- Kitamura M., Sakaguchi S., Sakaguchi G. Purification and some properties of Clostridium botulinum type-E toxin. Biochim Biophys Acta. 1968 Oct 21;168(2):207–217. doi: 10.1016/0005-2795(68)90144-x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lamanna C., Eklund H. W., McElroy O. E. Botulinum Toxin (Type A); Including a Study of Shaking with Chloroform as a Step in the Isolation Procedure. J Bacteriol. 1946 Jul;52(1):1–13. [PMC free article] [PubMed] [Google Scholar]
- Lamanna C., Glassman H. N. The Isolation of Type B Botulinum Toxin. J Bacteriol. 1947 Nov;54(5):575–584. doi: 10.1128/jb.54.5.575-584.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lamanna C., Sakaguchi G. Botulinal toxins and the problem of nomenclature of simple toxins. Bacteriol Rev. 1971 Sep;35(3):242–249. doi: 10.1128/br.35.3.242-249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MOLLER V., SCHEIBEL I. Preliminary report on the isolation of an apparently new type of CI. botulinum. Acta Pathol Microbiol Scand. 1960;48:80–80. doi: 10.1111/j.1699-0463.1960.tb04741.x. [DOI] [PubMed] [Google Scholar]
- REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
- Sakaguchi G., Sakaguchi S., Kondo H. Rapid bioassay for Clostridium botulinum type-E toxins by intravenous injection into mice. Jpn J Med Sci Biol. 1968 Dec;21(6):369–378. doi: 10.7883/yoken1952.21.369. [DOI] [PubMed] [Google Scholar]
- YPHANSTIS D. A. Rapid determination of molecular weights of peptides and preteins. Ann N Y Acad Sci. 1960 Aug 31;88:586–601. doi: 10.1111/j.1749-6632.1960.tb20055.x. [DOI] [PubMed] [Google Scholar]