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. 1974 Dec;28(6):1030–1032. doi: 10.1128/am.28.6.1030-1032.1974

Membrane Concentration of Infectious Bovine Rhinotracheitis Virus from Water1

S R Tschider 1, D L Berryhill 1, I A Schipper 1
PMCID: PMC186877  PMID: 4375434

Abstract

A membrane adsorption procedure was used to concentrate infectious bovine rhinotracheitis virus from 1-liter quantities of distilled water. Cellulose nitrate membrane filters (0.45-μm pore size) efficiently adsorbed this herpesvirus from water, and virus was recovered from the membrane by elution with 10 ml of fetal calf serum during sonic treatment. The average recovery rate was 70%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg G., Dahling D. R., Berman D. Recovery of small quantities of viruses from clean waters on cellulose nitrate membrane filters. Appl Microbiol. 1971 Oct;22(4):608–614. doi: 10.1128/am.22.4.608-614.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dulbecco R. Production of Plaques in Monolayer Tissue Cultures by Single Particles of an Animal Virus. Proc Natl Acad Sci U S A. 1952 Aug;38(8):747–752. doi: 10.1073/pnas.38.8.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kelling C. L., Schipper I. A., Strum G. E., Carlson R. B., Tilton J. E. Infectious bovine rhinotracheitis (IBR) abortion observations on incidence in vaccinated and non-vaccinated and exposed cattle. Cornell Vet. 1973 Jul;63(3):383–389. [PubMed] [Google Scholar]
  4. Konowalchuk J., Speirs J. I. An evaluation of three agents for eluting adsorbed enterovirus from millipore membrane filters. Can J Microbiol. 1971 Nov;17(11):1351–1355. doi: 10.1139/m71-216. [DOI] [PubMed] [Google Scholar]
  5. SCHROEDER R. J., MOYS M. D. An acute upper respiratory infection of dairy cattle. J Am Vet Med Assoc. 1954 Dec;125(933):471–472. [PubMed] [Google Scholar]
  6. Wallis C., Henderson M., Melnick J. L. Enterovirus concentration on cellulose membranes. Appl Microbiol. 1972 Mar;23(3):476–480. doi: 10.1128/am.23.3.476-480.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wallis C., Melnick J. L. Concentration of enteroviruses on membrane filters. J Virol. 1967 Jun;1(3):472–477. doi: 10.1128/jvi.1.3.472-477.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wallis C., Melnick J. L. Concentration of viruses on aluminum and calcium salts. Am J Epidemiol. 1967 May;85(3):459–468. doi: 10.1093/oxfordjournals.aje.a120708. [DOI] [PubMed] [Google Scholar]
  9. Wallis C., Melnick J. L., Fields J. E. Concentration and purification of viruses by adsorption to and elution from insoluble polyelectrolytes. Appl Microbiol. 1971 Apr;21(4):703–709. doi: 10.1128/am.21.4.703-709.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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