Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1975 Jan;29(1):17–20. doi: 10.1128/am.29.1.17-20.1975

Preparation of 14C-Labeled Sterigmatocystin in Liquid Media

Dennis P H Hsieh 1, Susie L Yang 1
PMCID: PMC186902  PMID: 1110489

Abstract

14C-labeled sterigmatocystin was prepared from surface cultures of Aspergillus versicolor A-18074 maintained in liquid media by multiple additions of [1-14C]acetate to the cultures. The highest yield of 7.75 mg/10 ml was found with a sucrose-asparagine-ammonium medium in which more than 3% of the radioactivity of the added [1-14C]acetate was recovered in the purified [ring-14C] sterigmatocystin. The method offers an easy way to prepare 14C-labeled sterigmatocystin for studies of this mycotoxin.

Full text

PDF
17

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biollaz M., Büchi G., Milne G. The biosynthesis of the aflatoxins. J Am Chem Soc. 1970 Feb 25;92(4):1035–1043. doi: 10.1021/ja00707a050. [DOI] [PubMed] [Google Scholar]
  2. Holzapfel C. W., Purchase I. F., Steyn P. S., Gouws L. The toxicity and chemical assay of sterigmatocystin, a carcinogenic mycotoxin, and its isolation from two new fungal sources. S Afr Med J. 1966 Dec 17;40(45):1100–1101. [PubMed] [Google Scholar]
  3. Hsieh D. P., Lin M. T., Yao R. C. Conversion of sterigmatocystin to aflatoxin B 1 by Aspergillus parasiticus. Biochem Biophys Res Commun. 1973 Jun 8;52(3):992–997. doi: 10.1016/0006-291x(73)91035-8. [DOI] [PubMed] [Google Scholar]
  4. Hsieh D. P., Mateles R. I. Preparation of labeled aflatoxins with high specific activities. Appl Microbiol. 1971 Jul;22(1):79–83. doi: 10.1128/am.22.1.79-83.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hsieh D. P., Mateles R. I. The relative contribution of acetate and glucose to aflatoxin biosynthesis. Biochim Biophys Acta. 1970 Jun;208(3):482–486. doi: 10.1016/0304-4165(70)90222-9. [DOI] [PubMed] [Google Scholar]
  6. Purchase I. F., Van Der Watt J. J. Carcinogenicity of sterigmatocystin. Food Cosmet Toxicol. 1970 Jun;8(3):289–295. doi: 10.1016/s0015-6264(70)80004-9. [DOI] [PubMed] [Google Scholar]
  7. Purchase I. F., van der Watt J. J. Carcinogenicity of sterigmatocystin. Food Cosmet Toxicol. 1968 Dec;6(4):555–556. doi: 10.1016/0015-6264(68)90186-7. [DOI] [PubMed] [Google Scholar]
  8. Reddy T. V., Viswanathan L., Venkitasubramanian T. A. High aflatoxin production on a chemically defined medium. Appl Microbiol. 1971 Sep;22(3):393–396. doi: 10.1128/am.22.3.393-396.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vorster L. J. A method for the analysis of cereals and groundnuts for three mycotoxins. Analyst. 1969 Feb;94(115):136–142. doi: 10.1039/an9699400136. [DOI] [PubMed] [Google Scholar]
  10. Vorster L. J., Purchase I. F. A method for the determination of sterigmatocystin in grain and oilseeds. Analyst. 1968 Oct;93(111):694–696. doi: 10.1039/an9689300694. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES