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. 1975 Jan;29(1):21–26. doi: 10.1128/am.29.1.21-26.1975

Development of a Simple Method for Concentrating Enteroviruses from Oysters

Mark D Sobsey 1,1, Craig Wallis 1, Joseph L Melnick 1
PMCID: PMC186903  PMID: 234154

Abstract

The development of a simple method for concentrating enteroviruses from oysters is described. In this method viruses in homogenized oyster tissues are efficiently adsorbed to oyster solids at pH 5.5 and low salt concentration. After low-speed centrifugation, the supernatant is discarded and viruses are eluted from the sedimented oyster solids by resuspending them in pH 3.5 glycine-buffered saline. The solids are then removed by low-speed centrifugation, and the virus-containing supernatant is filtered through a 0.2-μm porosity filter to remove bacteria and other small particulates without removing viruses. The virus-containing filtrate is then concentrated to a volume of a few milliliters by ultrafiltration, and the concentrate obtained is inoculated directly into cell cultures for virus assay. When tested with pools of oysters experimentally contaminated with small amounts of different enteroviruses, virus recovery efficiency averaged 63%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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