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Immunodetection of β-Pix in phosphotyrosine immunoprecipitates of CRP-stimulated platelets. Whole cell lysates and phosphotyrosine immunoprecipitated proteins were separated by 4-12% NuPAGE Bis-Tris gels and immunoblotted using an anti-β-Pix antibody. B, basal platelets; CRP, platelets stimulated with CRP (10 μg/mL, 90 sec); IP, immunoprecipitation; IB, immuno blot; WCL, whole cell lysate. Images represent at least three independent experiments.
